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  4. Recombinant Mouse Beta,beta-carotene 9',10'-oxygenase (Bco2)

Recombinant Mouse Beta,beta-carotene 9',10'-oxygenase (Bco2)

  • 中文名稱：
    Recombinant Mouse Beta,beta-carotene 9',10'-oxygenase(Bco2)
  • 貨號：
    CSB-YP857513MO
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Mouse Beta,beta-carotene 9',10'-oxygenase(Bco2)
  • 貨號：
    CSB-EP857513MO
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    Recombinant Mouse Beta,beta-carotene 9',10'-oxygenase(Bco2)
  • 貨號：
    CSB-EP857513MO-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Mouse Beta,beta-carotene 9',10'-oxygenase(Bco2)
  • 貨號：
    CSB-BP857513MO
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Mouse Beta,beta-carotene 9',10'-oxygenase(Bco2)
  • 貨號：
    CSB-MP857513MO
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    Bco2
  • Uniprot No.：
  • 別名：
    Bco2; Bcdo2Beta,beta-carotene 9',10'-oxygenase; EC 1.13.11.71; B-diox-II; Beta-carotene dioxygenase 2
  • 種屬：
    Mus musculus (Mouse)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-532
  • 氨基酸序列
    MLGPKQSLPC IAPLLTTAEE TLSAVSARVR GHIPEWLNGY LLRVGPGKFE FGKDRYNHWF DGMALLHQFR MERGTVTYKS KFLQSDTYKA NSAGGRIVIS EFGTLALPDP CKSIFERFMS RFEPPTMTDN TNVNFVQYKG DYYMSTETNF MNKVDIEMLE RTEKVDWSKF IAVNGATAHP HYDPDGTAYN MGNSYGPRGS CYNIIRVPPK KKEPGETIHG AQVLCSIAST EKMKPSYYHS FGMTKNYIIF VEQPVKMKLW KIITSKIRGK PFADGISWEP QYNTRFHVVD KHTGQLLPGM YYSMPFLTYH QINAFEDQGC IVIDLCCQDD GRSLDLYQLQ NLRKAGEGLD QVYELKAKSF PRRFVLPLDV SVDAAEGKNL SPLSYSSASA VKQGDGEIWC SPENLHHEDL EEEGGIEFPQ INYGRFNGKK YSFFYGCGFR HLVGDSLIKV DVTNKTLRVW REEGFYPSEP VFVPVPGADE EDSGVILSVV ITPNQSESNF LLVLDAKSFT ELGRAEVPVQ MPYGFHGTFV PI
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Asymmetrically cleaves beta-carotene at the 9',10' double bond resulting in the formation of beta-apo-10'-carotenal and beta-ionone. Besides beta-carotene, lycopene is also oxidatively cleaved. The apocarotenals formed by this enzyme may be the precursors for the biosynthesis of retinoic acid or exert unknown physiological effects.
  • 基因功能參考文獻：
    1. BCO2 regulates hypothalamic mitochondrial function, nutrient metabolism, and local oxidative stress and inflammation. PMID: 28482236
    2. Loss of BCO2 induces mitochondrial hyperactivation, mitochondrial stress, and changes of the mitochondrial proteome, leading to mitochondrial energy insufficiency. BCO2 appears to be critical for proper hepatic mitochondrial function. PMID: 27991717
    3. Role of beta-Carotene 9,10-Dioxygenase in Macular Pigment Metabolism PMID: 26307071
    4. Both lycopene and APO10LA supplementation were effective in reducing high saturated fat-induced liver steatosis in mice where BCO2 expression was absent. PMID: 25644347
    5. Data from knockout mice suggest that regulation of hepatic gene expression by dietary factors (here, supplementation with lycopene or tomato powder nutraceuticals) is dependent on Bco2. PMID: 24553694
    6. Pleiotropic actions of carotene-9',10'-monooxygenase supporting a strong role of these protein in regulating tissue carotenoid accumulation as well as tomato carotenoid-independent regulation of lipid metabolism. PMID: 24034573
    7. a stepwise cleavage by BCO2 and BCO1 with APO10ol as an intermediate could provide a mechanism to tailor asymmetric carotenoids such as beta-cryptoxanthin for vitamin A production. PMID: 24106281
    8. we found that the third family member, beta,beta-carotene-9',10'-oxygenase (BCDO2), is a mitochondrial carotenoid-oxygenase with broad substrate specificity PMID: 21106934

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  • 亞細胞定位：
    Mitochondrion.
  • 蛋白家族：
    Carotenoid oxygenase family
  • 數據庫鏈接：


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