Recombinant Mouse Aryl hydrocarbon receptor (Ahr), partial

中文名稱：

Recombinant Mouse Aryl hydrocarbon receptor (Ahr), partial
貨號：

CSB-EP001481MO1
規格：

￥1536
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

Greater than 85% as determined by SDS-PAGE.
生物活性：

Not Test
基因名：

Ahr
Uniprot No.：

P30561
種屬：

Mus musculus (Mouse)
蛋白長度：

Partial
來源：

E.coli
分子量：

41.3 kDa
表達區域：

101-400aa
氨基酸序列

RAQIRDWQDLQEGEFLLQALNGFVLVVTADALVFYASSTIQDYLGFQQSDVIHQSVYELIHTEDRAEFQRQLHWALNPDSAQGVDEAHGPPQAAVYYTPDQLPPENASFMERCFRCRLRCLLDNSSGFLAMNFQGRLKYLHGQNKKGKDGALLPPQLALFAIATPLQPPSILEIRTKNFIFRTKHKLDFTPIGCDAKGQLILGYTEVELCTRGSGYQFIHAADMLHCAESHIRMIKTGESGMTVFRLFAKHSRWRWVQSNARLIYRNGRPDYIIATQRPLTDEEGREHLQKRSTSLPFMF
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

C-terminal 6xHis-tagged
產品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

Ligand-activated transcription factor that enables cells to adapt to changing conditions by sensing compounds from the environment, diet, microbiome and cellular metabolism, and which plays important roles in development, immunity and cancer. Upon ligand binding, translocates into the nucleus, where it heterodimerizes with ARNT and induces transcription by binding to xenobiotic response elements (XRE). Regulates a variety of biological processes, including angiogenesis, hematopoiesis, drug and lipid metabolism, cell motility and immune modulation. Xenobiotics can act as ligands: upon xenobiotic-binding, activates the expression of multiple phase I and II xenobiotic chemical metabolizing enzyme genes (such as the CYP1A1 gene). Mediates biochemical and toxic effects of halogenated aromatic hydrocarbons. Next to xenobiotics, natural ligands derived from plants, microbiota, and endogenous metabolism are potent AHR agonists. Tryptophan (Trp) derivatives constitute an important class of endogenous AHR ligands. Acts as a negative regulator of anti-tumor immunity: indoles and kynurenic acid generated by Trp catabolism act as ligand and activate AHR, thereby promoting AHR-driven cancer cell motility and suppressing adaptive immunity. Regulates the circadian clock by inhibiting the basal and circadian expression of the core circadian component PER1. Inhibits PER1 by repressing the CLOCK-ARNTL/BMAL1 heterodimer mediated transcriptional activation of PER1. The heterodimer ARNT:AHR binds to core DNA sequence 5'-TGCGTG-3' within the dioxin response element (DRE) of target gene promoters and activates their transcription.
基因功能參考文獻：
1. Distribution of parent PCB congeners also varied by genotype with strikingly high levels of PCB 77 in poor-affinity Ahr (d) Cyp1a2(-/-) while Ahr (b) Cyp1a2(+/+) mice effectively sequestered coplanar PCBs in the liver. PMID: 29197979
2. these data support a prominent role for the AhR in regulating lung miRNA expression. PMID: 28079158
3. In air-exposed airway epithelia, induction of factors required for multiciliogenesis, including cyclin O (Ccno) and Multicilin (Mcidas), is AhR dependent, and air exposure induces AhR binding to the Ccno promoter. Submersion and hypoxic conditions impede AhR-dependent Ccno induction. PMID: 27554288
4. Shared epitope-aryl hydrocarbon receptor crosstalk underlies the mechanism of gene-environment interaction in autoimmune arthritis. PMID: 29666259
5. airway inflammatory cells and cytokines present in BALF and TRIF in lung tissue play a role in inducing AHR and airway inflammation upon RSV and bacteria coinfection PMID: 28695368
6. Ectopic activation of AHR during early differentiation disrupts the differentiation program via the chromatin remodeling complex NuRD. PMID: 29107595
7. AhR activation ameliorated epithelial barrier dysfunction following intestinal ischemia/reperfusion and hypoxia through upregulation of Notch1 signaling. PMID: 29286081
8. Low AHR expression is associated with provokes locomotor defects and alteration of myelin structure. PMID: 29351992
9. Data indicate aryl hydrocarbon receptor (AhR) and small heterodimer partner (SHP) as new physiological regulators of phosphatidylcholines (PC) and S-adenosylmethionine (SAM) levels. PMID: 29416063
10. These results suggested that AhR deficiency resulted in increased susceptibility to colitis, whereas activation of AhR by FICZ could ameliorate DSSinduced colitis via the MK2/pMK2/TTP pathway. PMID: 29207040
11. These results validate the role of the AhR pathway in regulating choroidal neovascular pathogenesis, identify mechanisms of AhR-based therapies in the eye, and argue in favor of developing AhR as a drug target for the treatment of neovascular AMD. PMID: 29481912
12. Deletion of the Ahr gene in cardiomyocytes protects males from heart dysfunction due to NKX2.5 haploinsufficiency. PMID: 28973413
13. Identify Cxcl5 as a novel target of AHR-mediated gene expression in primary mouse keratinocytes. PMID: 28973351
14. this study shows that tryptophan metabolite activation of the aryl hydrocarbon receptor regulates IL-10 receptor expression on intestinal epithelia PMID: 28098246
15. Ambient particulate matter enhances dendritic cell activation that primes naive T cell differentiation towards a Th17-like phenotype in an AhR-dependent manner. PMID: 29689377
16. AHR role in the acute lethal pulmonary inflammation PMID: 28673995
17. These findings highlight the physiologic importance of cell-type specific balancing of AhR/AhRR expression in response to microbial, nutritional and other environmental stimuli. Specifically, AhRR contributes to the maintenance of colonic intraepithelial lymphocytes and prevents excessive IL-1beta production and Th17/Tc17 differentiation. In contrast, the AhRR enhances IFN-gamma-production by effector T cells in the in... PMID: 27184933
18. these data support that RelB suppresses cigarette smoke-induced apoptosis, potentially by increasing the AhR PMID: 28254546
19. Results elucidate the dimerization process of AhR with ARNT and propose the structure of the N-terminal region of the AhR:ARNT dimer including the bHLH, PAS-A and PAS-B domains. PMID: 28393157
20. the AhR pathway is much more that a xenobiotic metabolism-inducing pathway, and the regulation by AhR of Notch signaling in the testis offers some insight into the mechanism of infertility in men. PMID: 27688768
21. UV and environmental stimulation of melanoblast-to-melanocyte maturation are enhanced via the AHR pathway. PMID: 27539901
22. the expression of CDC42 might be regulated by AHR, and both proteins are fundamental to the development of normal spermatozoa and the acrosome reaction. PMID: 27635527
23. study provides supportive evidence of the integration of AhR with circadian rhythms and metabolism, where its activation under homeostatic conditions influences circadian rhythm amplitude PMID: 28347186
24. the untimely expression of the Ahr gene needs to be repressed to maintain embryonic stem cell mitotic progression and prevent premature loss of pluripotency. PMID: 27374890
25. These results establish that mo-DCs and mo-Macs are controlled by distinct transcription factors and show that AHR acts as a molecular switch for monocyte fate specification in response to micro-environmental factors. PMID: 28930664
26. The results suggest AhR activation causes the loss of liver-specific and sexually dimorphic gene expression producing a functionally "de-differentiated" hepatic phenotype. PMID: 28922406
27. Study provides evidence supporting the importance of AHR in tumor progression and host survival through the upregulation of ISX expression and promotion of tumor cell growth while restraining the expression of tumor suppressors in hepatocellular carcinoma (HCC), and thereby suggests a plausible mechanistic link between AHR and HCC. PMID: 28398627
28. AhR controls COX-2 protein via mRNA stability. PMID: 28749959
29. functional AhR expression is critical for skin barrier integrity and that AhR represents a molecular target for the development of therapeutic approaches for skin barrier diseases, including by dietary intervention. PMID: 27430407
30. Lipopolysaccharide (LPS) stimulation increased the expression and activity of the immunoregulatory enzyme IDO1 in hepatic stellate cells (HSCs), and LPS/HSCs stimulated aryl hydrocarbon receptor (AhR) signaling in cocultured regulatory T cells. PMID: 27581538
31. Donor T cells lacking AhR demonstrate decreased acute graft versus host disease because of reduced donor T-cell proliferation early after transplant. PMID: 28550042
32. this study shows that AhR-/- mice display impaired bone healing with delayed endochondral ossification PMID: 27849171
33. AhR(-/-) mice are not able to mount an Natural killer cell memory response to hapten rechallenge. PMID: 27670593
34. Hyperhomocysteinemia activates the AHR-CD36 pathway by increasing hepatic LXA4 content, which results in hepatic steatosis. PMID: 26928949
35. data indicate that intestinal epithelial cells serve as gatekeepers for the supply of AHR ligands to the host and emphasize the importance of feedback control in modulating AHR pathway activation PMID: 28146477
36. Here, the authors report that Candida albicans infection activates the aryl hydrocarbon receptor (AhR), leading to activation of Src family kinases (SFKs), which in turn phosphorylate EGFR and induce endocytosis of the fungus. PMID: 28325761
37. AhR-deficient (Ahr(-/-)) B cells proliferate less than AhR-sufficient (Ahr(+/+)) cells following in vitro B-cell receptor stimulation and in vivo adoptive transfer models confirmed that Ahr(-/-) B cells are outcompeted by Ahr(+/+) cells. PMID: 27875245
38. AhR has a direct role in IL-22 production by Th17 cells in the mouse ear skin, but not by gammadelta T cells, CD4(-) CD8(-) TCRbeta(+) T cells and ILCs. PMID: 27000947
39. The inverse correlation between Smad7 and AhR expression helps to propagate inflammatory signals in the gut in Crohn's disease. PMID: 26818761
40. Findings suggest that miR-124 induces intestinal inflammation by inhibiting AHR to modulate pro-inflammatory cytokine production and thereby promotes the pathogenesis of Crohn's disease. PMID: 26802080
41. STAT3 may cooperate with AhR to regulate the differentiation of both Th17 and Treg cells PMID: 28445259
42. this study reveals the physiological importance of endogenous activation of AHR signaling in shaping the IFN-I-mediated innate response PMID: 27089381
43. AhR is associated with tumor prevention by regulating gut immunity, whereas in tumor cells, it is involved in growth suppression. PMID: 26973338
44. The co-treatment with gamma-T3 and baicalein enhanced the anti-proliferative activity of baicalein, accompanied by the downstream events of AhR-activation induced by baicalein. These data suggest that gamma-T3 upregulates AhR expression and enhances its sensitivity to baicalein. PMID: 27055589
45. Study elucidates that AhR plays an important role in mesangial cells activation, macrophage infiltration, and extracellular matrix accumulation in diabetic nephropathy conferred by oxidative stress. PMID: 26415004
46. data show that AHR contributes to hepatic energy homeostasis, partly through the regulation of FGF21 expression and signaling. PMID: 27226639
47. Indoleamine 2,3-dioxygenase 1 inhibitors can activate the aryl hydrocarbon receptor. PMID: 28336214
48. AhR expression is dependent on NFATc1 activation, and NFATc1 inhibition remarkably decreases AhR expression in mouse lung tissue. CsA administration resulted in a significant reduction of AhR expression. PMID: 27353300
49. this study shows that benzopyrene exposure increases Langerhans cell migration, and increases IL-5, IL-13, and IL-17 levels following atopic sensitization; the increased cytokine levels are attenuated in AhR knock-out mice PMID: 27129092
50. Disruption of the Ahr gene alters macrophage polarization when compared to WT macrophage PMID: 27153778
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亞細胞定位：

Cytoplasm. Nucleus.
組織特異性：

Expressed in all tissues tested including brain, heart, kidney, liver, lung, muscle, ovary, skin, spleen and thymus.
數據庫鏈接：

KEGG: mmu:11622

STRING: 10090.ENSMUSP00000112137

UniGene: Mm.341377