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Recombinant Mouse Alpha-defensin 1 (Defa1)

  • 中文名稱:
    Recombinant Mouse Alpha-defensin 1(Defa1)
  • 貨號:
    CSB-YP006652MO
  • 規格:
    ¥2208
  • 圖片:
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他:

產品詳情

  • 純度:
    Greater than 90% as determined by SDS-PAGE.
  • 基因名:
    Defa1
  • Uniprot No.:
  • 別名:
    Defa1; Defcr; Defcr1Alpha-defensin 1; Cryptdin-1; Defensin-related cryptdin peptide
  • 種屬:
    Mus musculus (Mouse)
  • 蛋白長度:
    Full Length of Mature Protein
  • 來源:
    Yeast
  • 分子量:
    31.1kDa
  • 表達區域:
    59-93aa
  • 氨基酸序列
    LRDLVCYCRSRGCKGRERMNGTCRKGHLLYTLCCR
    Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
    If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
  • 蛋白標簽:
    N-terminal GST-tagged
  • 產品提供形式:
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液:
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
    Note: If you have any special requirement for the glycerol content, please remark when you place the order.
    If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 儲存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 產品描述:

    The production of this Recombinant Mouse Defa1 protein required the insertion of a DNA fragment (Defa1, 59-93aa) into a plasmid vector and the transferral of this vector into Yeast cells (the step of transformation). The cells were then cultured and induced to express the Defa1 protein. This recombinant protein was fused with N-terminal GST tag. Its purity is 90%+ determined by SDS-PAGE.

    Defa1 (also called Defcr or Defcr1) is a gene providing an instruction of making a protein named alpha-defensin 1 (Defa1) in mus musculus (mouse). Defa1 protein is also known as cryptdin-1 or defensin-related cryptdin peptide and belongs to alpha-defensin (α-defensin) family. Mammalian α-defensins are cationic antimicrobial peptides (AMPs) with microbicidal activities against many species of bacteria (such as gram-negative and gram-positive bacterium) and fungi, antiviral functions, and diverse immunomodulatory effects (such as innate immune response in mucosa).

  • Datasheet & COA:
    Please contact us to get it.

產品評價

靶點詳情

  • 功能:
    Probably contributes to the antimicrobial barrier function of the small bowel mucosa. Has antibacterial activity against attenuated mutants of S.typhimurium.
  • 基因功能參考文獻:
    1. Mouse adenovirus 2 infection was enhanced by enteric alpha-defensins. PMID: 28622386
    2. Decreased alpha-defensin is associated with intestinal inflammation in a model of inflammatory bowel disease. PMID: 20222124
    3. cryptdin-1 and cryptdin-4 exhibit circadian oscillation PMID: 16129697
    4. the Arg5-Glu13 salt bridge in alpha-defensin is conserved for defensin in vivo stability PMID: 16246847
    5. MMP-7 activates mouse paneth cell pro-alpha-defensins PMID: 16822871

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  • 亞細胞定位:
    Secreted.
  • 蛋白家族:
    Alpha-defensin family
  • 組織特異性:
    Paneth cells of the small bowel.
  • 數據庫鏈接:

    KEGG: mmu:13216

    UniGene: Mm.425522



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