Recombinant Mouse Alpha-actinin-4 (Actn4), partial

中文名稱：

Recombinant Mouse Alpha-actinin-4 (Actn4), partial
貨號：

CSB-EP001244MO
規格：

￥1536
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

Greater than 85% as determined by SDS-PAGE.
生物活性：

Not Test
基因名：

Actn4
Uniprot No.：

P57780
種屬：

Mus musculus (Mouse)
蛋白長度：

Partial
來源：

E.coli
分子量：

37.2 kDa
表達區域：

1-267aa
氨基酸序列

MVDYHAANQAYQYGPNSGGGNGAGGGGSMGDYMAQEDDWDRDLLLDPAWEKQQRKTFTAWCNSHLRKAGTQIENIDEDFRDGLKLMLLLEVISGERLPKPERGKMRVHKINNVNKALDFIASKGVKLVSIGAEEIVDGNAKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYKNVNVQNFHISWKDGLAFNALIHRHRPELIEYDKLRKDDPVTNLNNAFEVAEKYLDIPKMLDAEDIVNTARPDEKAIMTYVSSFYH
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

C-terminal 6xHis-tagged
產品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation. Involved in tight junction assembly in epithelial cells probably through interaction with MICALL2. Links MICALL2 to the actin cytoskeleton and recruits it to the tight junctions. May also function as a transcriptional coactivator, stimulating transcription mediated by the nuclear hormone receptors PPARG and RARA.
基因功能參考文獻：
1. Focal segmental glomerulosclerosis Actn4 mutation promotes podocyte detachment through maladaptation to periodic stretch. PMID: 29378953
2. ACTN4 is a transcriptional coactivator of NF-kappaB. PMID: 25411248
3. sLZIP negatively regulates skeletal muscle differentiation via interaction with ACTN4. PMID: 24375477
4. the C-terminal tail of alpha-actinin-4 is essential for its function in cell migration and adhesion to substratum. PMID: 23466492
5. Dexamethasone mey protect actin cytoskeleton stabilization from adriamycin-induced podocyte injury via alpha-actinin-4. PMID: 22804863
6. anti-dsDNA Fab may enhance F-actin formation by the proprietary modification of alpha-actinin-4 PMID: 22929799
7. Together, these results indicate that alpha-actinin and the actin cytoskeleton are important components of the cisternal organelle that are probably required to stabilize the AIS. PMID: 21940706
8. Results suggest that ACTN4 is essential for maintaining normal spreading, motility, cellular and nuclear cross-sectional area, and contractility of lung fibroblasts by maintaining the balance between transcellular contractility and cell-adhesion. PMID: 21085685
9. newly developed mouse model of ACTN4-associated FSGS suggests a cause-and-effect relationship between actin cytoskeleton dysregulation by mutant alpha-actinin-4 and the deterioration of the nephrin-supported slit diaphragm complex. PMID: 12707390
10. nonsarcomeric alpha-actinin-4 is involved in the regulation of cell movement PMID: 12782671
11. ACTN4 has a role promoting tumorigenicity and regulating cell motility PMID: 15048094
12. FSGS-associated Actn4 impairs cytoskeletal dynamcs in podocytes. PMID: 16837921
13. alpha-actinin-4 interacts with integrins and strengthens the podocyte-GBM interaction thereby stabilizing glomerular architecture and preventing disease PMID: 17082197
14. The different genetic defects in Actn4 produce a spectrum of glomerular morphologic lesions depending on the specific combination of normal and/or defective alleles. PMID: 18185509
15. Although actinin-4 bound to JRAB/MICAL-L2 without Rab13, the actinin-4-JRAB/MICAL-L2 interaction was enhanced by Rab13 activation. PMID: 18332111
16. Alpha-actinin-4 K256E triggers several metabolic abnormalities, which may lead to glomerular epithelial cell injury and glomerulosclerosis. PMID: 19640905
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亞細胞定位：

Nucleus. Cytoplasm. Cell junction. Cytoplasm, cytoskeleton, stress fiber. Cytoplasm, perinuclear region.
蛋白家族：

Alpha-actinin family
數據庫鏈接：

KEGG: mmu:60595

STRING: 10090.ENSMUSP00000066068

UniGene: Mm.81144