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  4. Recombinant Mouse Actin, cytoplasmic 1 (Actb)

Recombinant Mouse Actin, cytoplasmic 1 (Actb)

  • 中文名稱：
    Recombinant Mouse Actin, cytoplasmic 1(Actb)
  • 貨號(hào)：
    CSB-YP001207MO
  • 規(guī)格：
  • 來(lái)源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Mouse Actin, cytoplasmic 1(Actb)
  • 貨號(hào)：
    CSB-EP001207MO
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 其他：
  • 中文名稱：
    Recombinant Mouse Actin, cytoplasmic 1(Actb)
  • 貨號(hào)：
    CSB-EP001207MO-B
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Mouse Actin, cytoplasmic 1(Actb)
  • 貨號(hào)：
    CSB-BP001207MO
  • 規(guī)格：
  • 來(lái)源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Mouse Actin, cytoplasmic 1(Actb)
  • 貨號(hào)：
    CSB-MP001207MO
  • 規(guī)格：
  • 來(lái)源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 別名：
    ActbActin; cytoplasmic 1; Beta-actin) [Cleaved into: Actin; cytoplasmic 1; N-terminally processed]
  • 種屬：
    Mus musculus (Mouse)
  • 蛋白長(zhǎng)度：
    Full length protein
  • 表達(dá)區(qū)域：
    1-375
  • 氨基酸序列
    MDDDIAALVV DNGSGMCKAG FAGDDAPRAV FPSIVGRPRH QGVMVGMGQK DSYVGDEAQS KRGILTLKYP IEHGIVTNWD DMEKIWHHTF YNELRVAPEE HPVLLTEAPL NPKANREKMT QIMFETFNTP AMYVAIQAVL SLYASGRTTG IVMDSGDGVT HTVPIYEGYA LPHAILRLDL AGRDLTDYLM KILTERGYSF TTTAEREIVR DIKEKLCYVA LDFEQEMATA ASSSSLEKSY ELPDGQVITI GNERFRCPEA LFQPSFLGME SCGIHETTFN SIMKCDVDIR KDLYANTVLS GGTTMYPGIA DRMQKEITAL APSTMKIKII APPERKYSVW IGGSILASLS TFQQMWISKQ EYDESGPSIV HRKCF
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells. Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction. In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA.
  • 基因功能參考文獻(xiàn)：
    1. Data, including data from studies using knockout mice, suggest that Actb and Actg1 are enriched in isolated membrane preparations from skeletal muscle which represent interface between mitochondria and sarco-endoplasmic reticulum; these structures appear to be important in signaling, mitochondrial dynamics, and muscle relaxation. (Actb = beta actin cytoplasmic 1; Actg1 = actin gamma cytoplasmic 1) PMID: 29265728
    2. Essential nucleotide- and protein-dependent functions of Actb/beta-actin. PMID: 30012594
    3. These results suggest a a novel genome-wide mechanism where the polymerase-associated beta-actin synergizes with NM1 to coordinate permissive chromatin with Pol I transcription, cell growth, and proliferation. PMID: 27127100
    4. Two actin isoforms, beta- and gamma-actin, are crucial for slow, rapid, bulk, and overshoot endocytosis at large calyx-type synapses, and for slow endocytosis and bulk endocytosis at small hippocampal synapses. PMID: 27840001
    5. Melatonin treatment for 1 h increased monomeric actin and decreased polymeric actin. PMID: 28209510
    6. Disruption of the in vivo interaction of KIF11 with ZBP1 delocalizes beta-actin mRNA and affects cell migration. PMID: 25588836
    7. TIA proteins can function as long-term regulators of the ACTB mRNA metabolism in mouse and human cells. PMID: 24766723
    8. Actin binding proteins associated with stress fiber or focal adhesion formation are overexpressed in the beta-actin knock-out cells and many of these contain CH-, LIM- or EFh- domains. PMID: 24123846
    9. Absence of beta-actin severely hampers formation of the peripheral nervous system, due to defective neural crest migration. PMID: 24409333
    10. Data indicate that ectopic phosphatidylinositol 3,4,5-triphosphate (PIP3) elevation alone activated membranes to form actin-based structures whose behavior was similar to that of growth-cone-like "waves". PMID: 23951027
    11. this work demonstrated that dendritic beta-actin messenger RNA and ribosomes are in a masked, neuron-specific form. PMID: 24458642
    12. chorein interacts with beta-adducin and beta-actin. PMID: 24129186
    13. RNA polymerase II, glyceraldehyde-3-phosphate dehydrogenase (Gapdh), and hypoxanthine phophoribosyltransferase (Hprt) show a more stable gene expression as candidate genes than do ribosomal 18s and Actb. PMID: 22735032
    14. The findings suggested that local translation of beta-actin in growth cones of motoneurons is regulated by laminin signalling and that this signalling is disturbed in survival of motoneuron protein-deficient motoneurons. PMID: 22810847
    15. Data suggest that in beta-actin knockout cells other actins compensate for beta-actin in cell migration process. PMID: 22448045
    16. Data indicate that Ubc and Ywhaz were best correlated for B cells and lymphocytes, whereas Ubc and Gapdh were the best combination for non-B cells, and Actb and Hprt1 were the least stably expressed genes for B cells and non-B cell. PMID: 22884776
    17. the zipcode-binding protein 1 transports beta-actin mRNA to the focal adhesion compartment, it dwells for minutes, associating its localization with motility through the formation of stable connections between adhesions and newly synthesized actin filaments PMID: 22948660
    18. These data show that thymosinbeta4 and beta-actin are over-expressed during nurse cell formation upon T. spiralis infection and may be involved in nurse cell formation along with other actin-binding proteins. PMID: 22305657
    19. novel roles for beta-actin in promoting complex CNS tissue architecture PMID: 22403730
    20. Beta- and gamma-cytoplasmic actins are required for meiosis in mouse oocytes. PMID: 21778137
    21. These data support an essential role for beta-actin in regulating cell migration and gene expression through control of the cellular G-actin pool PMID: 21900491
    22. Perturbing the dystrophin and beta actin interaction results in quadriceps myopathy. PMID: 21325027
    23. Nuclear beta-actin controls growth arrest of epithelial cells. PMID: 21172822
    24. Results demonstrate overlapping developmental roles but unique post-developmental functions for beta-actin and gamma-actin in maintaining hair cell stereocilia. PMID: 20976199
    25. Data show that exposure to hyperoxia resulted in significant increases in eNOS-beta-actin association, eNOS activity, and protein tyrosine nitration in the lungs. PMID: 20826796
    26. arginylated gamma-actin, unlike beta-, was highly unstable and selectively ubiquitinated and degraded in vivo; instability was regulated by differences in nucleotide coding sequence between the actin isoforms, which conferred different translation rates PMID: 20847274
    27. HnRNP-R-mediated axonal beta-actin mRNA translocation plays an essential physiological role for axon growth and presynaptic differentiation. PMID: 20167579
    28. Dystrophin and utrophin bind actin through distinct modes of contact PMID: 16478721
    29. beta-actin was arginylated in vivo to regulate actin filament properties, beta-actin localization, and lamella formation in motile cells PMID: 16794040
    30. Data show that beta-actin characteristic markers of brush borders, become abnormally distributed along these atypical basolateral membranes in gal3(-/-) mice. PMID: 18211959
    31. The cytoplasmic beta-actin (Actb) gene generates two alternative transcripts terminated at tandem polyA sites. PMID: 18835850
    32. Ischemia-mediated aggregation of the actin cytoskeleton is one of the major initial events resulting in ischemia-reperfusion injury. PMID: 19095765

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  • 亞細(xì)胞定位：
    Cytoplasm, cytoskeleton. Nucleus.
  • 蛋白家族：
    Actin family
  • 組織特異性：
    Expressed in the epididymis (at protein level). Expressed in the kidney (at protein level).
  • 數(shù)據(jù)庫(kù)鏈接：


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