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  4. Recombinant Mouse 7SK snRNA methylphosphate capping enzyme (Mepce)

Recombinant Mouse 7SK snRNA methylphosphate capping enzyme (Mepce)

  • 中文名稱：
    小鼠Mepce重組蛋白
  • 貨號：
    CSB-YP810387MO
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    小鼠Mepce重組蛋白
  • 貨號：
    CSB-EP810387MO
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    小鼠Mepce重組蛋白
  • 貨號：
    CSB-EP810387MO-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    小鼠Mepce重組蛋白
  • 貨號：
    CSB-BP810387MO
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    小鼠Mepce重組蛋白
  • 貨號：
    CSB-MP810387MO
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 別名：
    Mepce; Bcdin3; Bipl1; D5Wsu46e7SK snRNA methylphosphate capping enzyme; MePCE; EC 2.1.1.-
  • 種屬：
    Mus musculus (Mouse)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-666
  • 氨基酸序列
    MIEMAAEKEP FLVPAPPPPL KDESGGGGGP EVQSHQEAAS GELRDGTEHG PGPRAHSAGA AASGGGGPQA QAHGEPHGRA AAPADVGEER RGGGGTDLGP PAPPRPRNGY QPHRPPGGGG GKRRNSCNVG GGSGGSFKHP AFKRRRRVNS DCDSVLPSNF LLGGNIFDPL NLNSLLDEEV SRALNAETPK SSPLPAKGRD PVEILIPKDI TDPLSLNTCT DEAHVVLASP LKIGRKRHRH RGPHHQQQQQ ASGGNDSNAA VLPTDPLTPS LHGEGATQQQ QNRGQNRDAP QPYELNTAIN CRDEVVSPLP SALQGSSGSL SAPPAASVTS APSTSSSSRH RKRRRTSSKS EAGARGGSQG SKEKGRGSGG GRHHHHPLPA TGFKKQQLKF QYGNYCKYYG YRNPSCEDVR LRVLKPEWFQ GRDVLDLGCN VGHLTLSIAC KWGPARMVGL DIDPRLIHSA RQNIRHYLSE ELRLQAQTSE GDPGTEGEEG TITVRKRSCF PASLTASRGP IAAPQVPLDG ADTSVFPNNV VFVTGNYVLD RDELVDAQRP EYDVVLCFSL TKWVHLNWGD EGLKRMFRRI YRHLRPGGIL VLEPQPWSSY CKRKSLTETI YKNYFRIQLK PEQFSSYLTS PEVGFSSYEL VATPNNTSRG FQRPVYLFHK ARSPSH
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    S-adenosyl-L-methionine-dependent methyltransferase that adds a methylphosphate cap at the 5'-end of 7SK snRNA (7SK RNA), leading to stabilize it. Also has a non-enzymatic function as part of the 7SK RNP complex: the 7SK RNP complex sequesters the positive transcription elongation factor b (P-TEFb) in a large inactive 7SK RNP complex preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation. The 7SK RNP complex also promotes snRNA gene transcription by RNA polymerase II via interaction with the little elongation complex (LEC). In the 7SK RNP complex, MEPCE is required to stabilize 7SK RNA and facilitate the assembly of 7SK RNP complex. MEPCE has a non-enzymatic function in the 7SK RNP complex; it has a non-enzymatic function; interaction with LARP7 within the 7SK RNP complex occluding its catalytic center.
  • 亞細胞定位：
    Nucleus.
  • 蛋白家族：
    Methyltransferase superfamily
  • 數據庫鏈接：


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