Recombinant Mouse 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial (Cyp27b1)

中文名稱：

Recombinant Mouse 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial(Cyp27b1)
貨號：

CSB-EP006406MO
規格：

￥2328
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

Greater than 90% as determined by SDS-PAGE.
基因名：

Cyp27b1
Uniprot No.：

O35084
別名：

Cyp27b1; Cyp27b; Cyp4025-hydroxyvitamin D-1 alpha hydroxylase; mitochondrial; EC 1.14.15.18; 25-OHD-1 alpha-hydroxylase; 25-hydroxyvitamin D(3) 1-alpha-hydroxylase; VD3 1A hydroxylase; Calcidiol 1-monooxygenase; Cytochrome P450 subfamily XXVIIB polypeptide 1; Cytochrome P450C1 alpha; Cytochrome P450VD1-alpha; Cytochrome p450 27B1
種屬：

Mus musculus (Mouse)
蛋白長度：

Full Length
來源：

E.coli
分子量：

60.2kDa
表達區域：

1-507aa
氨基酸序列

MTQAVKLASRVFHRIHLPLQLDASLGSRGSESVLRSLSDIPGPSTLSFLAELFCKGGLSRLHELQVHGAARYGPIWSGSFGTLRTVYVADPTLVEQLLRQESHCPERCSFSSWAEHRRRHQRACGLLTADGEEWQRLRSLLAPLLLRPQAAAGYAGTLDNVVRDLVRRLRRQRGRGSGLPGLVLDVAGEFYKFGLESIGAVLLGSRLGCLEAEVPPDTETFIHAVGSVFVSTLLTMAMPNWLHHLIPGPWARLCRDWDQMFAFAQRHVELREGEAAMRNQGKPEEDMPSGHHLTHFLFREKVSVQSIVGNVTELLLAGVDTVSNTLSWTLYELSRHPDVQTALHSEITAGTRGSCAHPHGTALSQLPLLKAVIKEVLRLYPVVPGNSRVPDRDIRVGNYVIPQDTLVSLCHYATSRDPTQFPDPNSFNPARWLGEGPTPHPFASLPFGFGKRSCIGRRLAELELQMALSQILTHFEVLPEPGALPIKPMTRTVLVPERSINLQFVDR
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 6xHis-tagged
產品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

A cytochrome P450 monooxygenase involved in vitamin D metabolism and in calcium and phosphorus homeostasis. Catalyzes the rate-limiting step in the activation of vitamin D in the kidney, namely the hydroxylation of 25-hydroxyvitamin D3/calcidiol at the C1-alpha position to form the hormonally active form of vitamin D3, 1alpha,25-dihydroxyvitamin D3/calcitriol that acts via the vitamin D receptor (VDR). Has 1-alpha-hydroxylase activity on vitamin D intermediates of the CYP24A1-mediated inactivation pathway. Converts 24R,25-dihydroxyvitamin D3/secalciferol to 1-alpha,24,25-trihydroxyvitamin D3, an active ligand of VDR. Also active on 25-hydroxyvitamin D2. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via FDXR/adrenodoxin reductase and FDX1/adrenodoxin.
基因功能參考文獻：
1. Vascular Calcification Induced by Chronic Kidney Disease Is Mediated by an Increase of 1alpha-Hydroxylase Expression in Vascular Smooth Muscle Cells PMID: 27074284
2. These results reveal that differential regulation of Cyp27b1 expression represents a mechanism whereby 1,25(OH)2D3 can fulfill separate functional roles, first in the kidney to control mineral homeostasis and second in extra-renal cells to regulate target genes linked to specific biological responses. PMID: 28808057
3. The data indicate that abnormal osteoclastogenesis due to the absence of CYP27B1 expression, consistent with the notion that endogenous metabolism of 25-hydroxyvitamin D optimizes osteoclastogenesis and ameliorates the resulting activity of mature osteoclasts. PMID: 26639637
4. The absence of 25-hydroxyvitamin D3-1alpha-hydroxylase potentiates the suppression of EAE in mice by ultraviolet light. PMID: 27108944
5. Cyp27b1(-/-) mice exhibited hypocalcemia, growth defects, and skeletogenesis dysfunction, similar to Vdr(-/-) mice, but do not display alopecia PMID: 28025137
6. Findings demonstrate that in-tumor CYP27B1 1-alpha-hydroxylase activity plays a crucial role in controlling early oncogene-mediated mammary carcinogenesis events, at least in part by modulating tumoral cell NF-kappaB p65 nuclear translocation. PMID: 27119753
7. the effect of 25-hydroxyvitamin D-1-alpha-hydroxylase on the atherosclerosis disease both in apolipoprotein (apo) E-/- mice and wild-type mice, was investigated. PMID: 28178628
8. P450C1 alpha deficiency results in abnormal calcium handling and cardiac dysfunction in mice with defective vitamin D signaling. PMID: 25268137
9. Throughout the male reproductive tract specific bands of CYP27B1 are determined. PMID: 23188491
10. observed a 3- to 10-fold increase in CYP27B1 mRNA abundance in the lung, spleen, aorta and testis of FGF-23 null/1alpha-Luc mice PMID: 24019880
11. Data show that the absence of either of the two key hydroxylases, vitamin D 25-hydroxylase (CYP2R1) or vitamin D 25-hydroxyvitamin D-1alpha-hydroxylase (CYP27B1)neither inhibits nor enhances the development of experimental autoimmune encephalomyelitis (EAE). PMID: 22592802
12. These results confirm the expression of vitamin D receptor and Cyp27b1 in vivo and suggest a potential role for vitamin D(3) in skeletal muscle regeneration following injury. PMID: 22648952
13. Bone formation parameters were increased significantly in all pups fed by dams on the rescue diet but were still lower in 1alpha(OH)ase(-/-) pups than in 1alpha(OH)ase(+/-) pups. PMID: 21791625
14. 1alpha-hydroxylase may have a role in enhanced radiosensitivity PMID: 21343672
15. regulation of bone CYP27B1 is unique from that in the kidney, and may play an important role in bone formation PMID: 20236619
16. study shows CYP27B1 can hydroxylate 25-hydroxyvitamin D2 & 25-hydroxyvitamin D3 associated with phospholipid membranes; low activity found at higher membrane levels of 25-hydroxyvitamin D; substrate inhibition may contribute to enzyme activity regulation PMID: 20193763
17. both 1alphaOHase gene ablation and Pi supplementation inhibit renal calcification in Npt2-/- mice and that 1,25(OH)2D is essential for the development of hypercalciuria and nephrocalcinosis in the mutant strain. PMID: 14656762
18. leptin suppresses renal gene overexpression for 1 alpha-hydroxylase and 24-hydroxylase and corrects increased serum concentrations of calcium and phosphate in ob/ob mice. PMID: 14657008
19. 1 alpha-OHase knockout mice fed a normal Ca2+ diet developed severe hypocalcemia, rickets and died with an average life span of 12 +/- 2 weeks PMID: 14717923
20. 25OHD 1OHase essential for normal epidermal differentiation, most likely by producing vitamin D metabolite, 1,25(OH)(2)D, responsible for inducing proteins regulating calcium levels in epidermis critical for generation and maintenance of barrier. PMID: 15102089
21. 5' flanking region of the gene directs expression to the proximal convoluted tubules of the kidney PMID: 15691891
22. study of substrate recognition of CYP27B1 PMID: 15972816
23. CYP27B1 null mice with LacZreporter gene display no 25-hydroxyvitamin D3-1alpha-hydroxylase promoter activity in the skin. PMID: 16371465
24. These results suggest that adrenodoxin functions as an effector for the oxygen transfer reaction in addition to being an electron donor for CYP27B1. PMID: 16584176
25. confirm a crucial role for STAT1alpha as well as for C/EBPbeta in the regulation of 1alpha-hydroxylase in monocytes PMID: 17267208
26. leptin attenuates renal 1alpha-hydroxylase gene expression through ObRb. Furthermore, leptin appears to act indirectly on renal proximal tubules to regulate 1alpha-hydroxylase gene expression. PMID: 17400175
27. FGF-23 directly regulates renal 1alpha-hydroxylase gene expression via activation of the ERK1/2 signaling pathway. PMID: 17699549
28. Neither high dietary Ca nor high dietary vitamin D3 is sufficient to fully recover the phenotype of CYP27B1 KO mice. PMID: 18029472
29. Results demonstrate that the proximal 1.5kb 5' flanking region of the CYP27B1 gene directs the expression of CYP27B1 in a number of known and novel tissues in a specific manner. PMID: 18313834
30. Lack of Cyp27b1 exacerbates disease in mouse model, suggesting that similar effects may occur with vitamin D deficiency PMID: 18535110
31. 1alpha-hydroxylase is expressed in adipose tissue and is functional in cultured adipocytes. PMID: 18840526
32. Results from genetic manipulation of expression of Cyp27b1 in chondrocytes support direct role for locally synthesized 1,25(OH)2D3, acting through the vitamin D receptor, in vascular invasion & osteoclastogenesis during endochondral bone development. PMID: 19477943
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亞細胞定位：

Mitochondrion membrane.
蛋白家族：

Cytochrome P450 family
組織特異性：

Kidney.
數據庫鏈接：

KEGG: mmu:13115

STRING: 10090.ENSMUSP00000130005

UniGene: Mm.6216