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  4. Recombinant Mitogen-activated protein kinase mpk-1 (mpk-1)

Recombinant Mitogen-activated protein kinase mpk-1 (mpk-1)

  • 中文名稱：
    秀麗隱桿線蟲(chóng)mpk-1重組蛋白
  • 貨號(hào)：
    CSB-YP340147CXY
  • 說(shuō)明書(shū)：
  • 規(guī)格：
  • 來(lái)源：
    Yeast
  • 其他：
  • 中文名稱：
    秀麗隱桿線蟲(chóng)mpk-1重組蛋白
  • 貨號(hào)：
    CSB-EP340147CXY
  • 說(shuō)明書(shū)：
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 其他：
  • 中文名稱：
    秀麗隱桿線蟲(chóng)mpk-1重組蛋白
  • 貨號(hào)：
    CSB-EP340147CXY-B
  • 說(shuō)明書(shū)：
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    秀麗隱桿線蟲(chóng)mpk-1重組蛋白
  • 貨號(hào)：
    CSB-BP340147CXY
  • 說(shuō)明書(shū)：
  • 規(guī)格：
  • 來(lái)源：
    Baculovirus
  • 其他：
  • 中文名稱：
    秀麗隱桿線蟲(chóng)mpk-1重組蛋白
  • 貨號(hào)：
    CSB-MP340147CXY
  • 說(shuō)明書(shū)：
  • 規(guī)格：
  • 來(lái)源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    mpk-1
  • Uniprot No.：
  • 別名：
    mpk-1; sur-1; F43C1.2; Mitogen-activated protein kinase mpk-1; EC 2.7.11.24; MAP kinase sur-1
  • 種屬：
    Caenorhabditis elegans
  • 蛋白長(zhǎng)度：
    full length protein
  • 表達(dá)區(qū)域：
    1-444
  • 氨基酸序列
    MPTWIPNNLC AQPTTRNAKP PSNGHPQATQ QQSAPGSLAY RNSSNIPNGA TNHVRQQKWQ YTRSGHRKMA DGEAVISTVN NVEEVHGQLF EVAPRYVNLS YIGEGAYGMV ASALDTITRD RVAIKKISPF EHQTFCQRTL REIKILNRFK HENIINIQEI IRSETVDSLK DIYIVQCLME TDLYKLLKTQ KLSNDHVCYF LYQILRGLKY IHSANVLHRD LKPSNLLLNT TCDLKICDFG LARVTDPQTD HTGFLTEYVA TRWYRAPEIM LNSKGYTKSI DVWSVGCILA EMLSNRPLFP GKHYLDQLNL ILAVVGSPSN ADLQCIINDK ARSYLISLPH KPKQPWARLY PGADPRALDL LDKMLTFNPH NRIDIEQALA HPYLEQYYDP GDEPVCEEPF TLEMEFDDLP KEKLKELIWE EAEAHHRRME AEAAARNNGG QNPV
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Function in let-60 Ras signaling pathway; acts downstream of lin-45 raf kinase, but before the lin-1 gene product in controlling vulval cell differentiation. Plays a negative role in proximal germline proliferation in the mitotic zone. Required for progression of developing oocytes through the pachytene stage. In oocytes, inhibits the activity of the chloride channel clh-3, likely by activating gck-3. Plays a role in response to M.nematophilum-mediated bacterial infection by promoting tail swelling and preventing constipation. Involved in fluid homeostasis. In addition, involved in the up-regulation of lysozyme ilys-3 expression in the intestine in responses to M.nematophilum-mediated bacterial infection. By phosphorylating transcription factor skn-1 (isoform c) may play a role in increasing life span downstream of lin-45, let-60 and mek-2. By up-regulating cep-1 and down-regulating gld-1 expression in the late pachytene stage, plays a role in germline apoptosis in response to DNA damage. Regulates egl-1 expression in response to DNA damage, probably upstream of cep-1.; Suppresses germline tumor formation by preventing the dedifferentiation of secondary spermatocytes probably upstream of rskn-1.
  • 基因功能參考文獻(xiàn)：
    1. The results suggest that the MPK-1/ERK regulatory network, including FBF-1, FBF-2, and LIP-1, controls the number of sperm by regulating the timing of the sperm-oocyte switch in C. elegans. PMID: 28782521
    2. data suggest that KIN-18 coordinates meiotic progression by modulating the timing of MPK-1 activation and the progression of recombination events PMID: 26510792
    3. We show that MPK-1 signaling plays important developmental roles in pachytene progression and in regulating CEP-1 expression in pachytene, and a possible direct role in DNA damage induced apoptosis PMID: 21901106
    4. In the proximal germline, sperm-dependent physiological MPK-1 activation results in phosphorylation-based inactivation of NOS-3, FEM-CUL-2-mediated degradation of TRA-1 and the promotion of membrane organization during oogenesis. PMID: 21571224
    5. C. elegans ERK kinase MPK-1 or ERK phosphorylating/activating kinase MEK-2 constitutively activates native ClC anion channel CLH-3b. PMID: 21160027
    6. Data indicates that MAP kinase signaling antagonizes PAR-1 signaling during early embryonic polarization. PMID: 19720857
    7. GCK-1 is a negative regulator of MAP kinase activation and apoptosis in the C. elegans germline PMID: 19826475
    8. MPK-1 thus may act not only in the control of individual processes but also in the coordination of contemporaneous processes and the integration of sequential processes in germline development PMID: 18073423

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  • 蛋白家族：
    Protein kinase superfamily, CMGC Ser/Thr protein kinase family, MAP kinase subfamily
  • 組織特異性：
    Expressed in cells lining the rectum. Isoform a is expressed in nervous system, body wall muscles and posterior intestine. Isoform b expression may be restricted to germline.
  • 數(shù)據(jù)庫(kù)鏈接：

    KEGG: cel:CELE_F43C1.2

    STRING: 6239.F43C1.2b

    UniGene: Cel.34032



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