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  4. Recombinant Methanocaldococcus jannaschii Uncharacterized protein MJ0384 (MJ0384)

Recombinant Methanocaldococcus jannaschii Uncharacterized protein MJ0384 (MJ0384)

  • 中文名稱：
    Recombinant Methanocaldococcus jannaschii Uncharacterized protein MJ0384(MJ0384)
  • 貨號：
    CSB-YP691001MRU
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Methanocaldococcus jannaschii Uncharacterized protein MJ0384(MJ0384)
  • 貨號：
    CSB-EP691001MRU
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    Recombinant Methanocaldococcus jannaschii Uncharacterized protein MJ0384(MJ0384)
  • 貨號：
    CSB-EP691001MRU-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Methanocaldococcus jannaschii Uncharacterized protein MJ0384(MJ0384)
  • 貨號：
    CSB-BP691001MRU
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Methanocaldococcus jannaschii Uncharacterized protein MJ0384(MJ0384)
  • 貨號：
    CSB-MP691001MRU
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    cas3
  • Uniprot No.：
  • 別名：
    cas3; cas3''; MJ0384CRISPR-associated endonuclease Cas3-HD; EC 3.1.-.-; CRISPR-associated ssDNA/ssRNA endonuclease Cas3-HD
  • 種屬：
    Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-244
  • 氨基酸序列
    MILGEIMEVL AFKNQSLIDH VNDMVKYWER IKYRYLKTIK RALEALNIKL DIEKVDEFMK ILIKLHDIGK ASKIYQRAII NDQEKLMGFR HELVSAYYTY HILLKKFGDK NLAFIGALTV MLHHEPIIMG QIRNLKKKEL TAEVVLDKLK KFDGMIEDFE DLIKKLIGYS IGDIIKNDSN KDDIIRFVIE MSVRARHTPN SEKLRFIVGT LLLPLVMCDY KGAESREGKA PKFAEVLEVE SYVI
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Cas3 plus Cascade participate in CRISPR interference, the third stage of CRISPR immunity. Acts as a ssDNA and ssRNA nuclease, with both endo- and 3' to 5' exonuclease activities, acting on substrates with free single-stranded 3' ends. Double-stranded nucleic acids are not substrates. Activity is higher for DNA than RNA. Templates include R-loops (a bubble-like structure formed when ssRNA replaces one strand in a dsDNA, such as crRNA is thought to form with CRISPR target DNA), circular ssDNA, 2',3'-cAMP and 2',3'-cGMP. Probably generates 3'-phosphate and 5'-hydroxyl ends. In the presence of the Cas3 helicase and ATP dsDNA templates are also degraded.
  • 蛋白家族：
    CRISPR-associated nuclease Cas3-HD family
  • 數據庫鏈接：

    KEGG: mja:MJ_0384

    STRING: 243232.MJ_0384



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