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  4. Recombinant Meiotic spindle formation protein mei-1 (mei-1)

Recombinant Meiotic spindle formation protein mei-1 (mei-1)

  • 中文名稱：
    秀麗隱桿線蟲mei-1重組蛋白
  • 貨號：
    CSB-YP330397CXY
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    秀麗隱桿線蟲mei-1重組蛋白
  • 貨號：
    CSB-EP330397CXY
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    秀麗隱桿線蟲mei-1重組蛋白
  • 貨號：
    CSB-EP330397CXY-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    秀麗隱桿線蟲mei-1重組蛋白
  • 貨號：
    CSB-BP330397CXY
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    秀麗隱桿線蟲mei-1重組蛋白
  • 貨號：
    CSB-MP330397CXY
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    mei-1
  • Uniprot No.：
  • 別名：
    mei-1; T01G9.5Meiotic spindle formation protein mei-1; EC 5.6.1.1; Katanin p60 ATPase-containing subunit A1; Katanin p60 subunit A1; p60 katanin
  • 種屬：
    Caenorhabditis elegans
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-472
  • 氨基酸序列
    MNGDVQSVIR GYLERAQVAK TMSDAGRWNE AGDLLRQLMT DVKSCKISAS NRDEHDARNT FLRALEANLK LVQQNVRDED DLHEAMTRQS GSPEPPADPD VWSKPSPPLP SSSKFGATKK GVGAAGPRPR EISKSTSSMS TNPADVKPAN PTQGILPQNS AGDSFDASAY DAYIVQAVRG TMATNTENTM SLDDIIGMHD VKQVLHEAVT LPLLVPEFFQ GLRSPWKAMV LAGPPGTGKT LIARAIASES SSTFFTVSST DLSSKWRGDS EKIVRLLFEL ARFYAPSIIF IDEIDTLGGQ RGNSGEHEAS RRVKSEFLVQ MDGSQNKFDS RRVFVLAATN IPWELDEALR RRFEKRIFIP LPDIDARKKL IEKSMEGTPK SDEINYDDLA ARTEGFSGAD VVSLCRTAAI NVLRRYDTKS LRGGELTAAM ESLKAELVRN IDFEAALQAV SPSAGPDTML KCKEWCDSFG AM
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Catalytic subunit of a complex which severs microtubules in an ATP-dependent manner. Microtubule severing may promote rapid reorganization of cellular microtubule arrays. Required specifically for meiotic spindle formation in the female germline; the presence of this protein is inimical to the formation of mitotic spindles. In body wall muscles, regulates organization of myosin thick filaments.
  • 基因功能參考文獻：
    1. The reconstructions show that katanin cycles between open spiral and closed ring conformations, depending on the ATP occupancy of a gating protomer that tenses or relaxes interprotomer interfaces. PMID: 28783150
    2. mei-1 and aspm-1 promote oocyte meiotic spindle pole assembly PMID: 24554763
    3. The phosphorylation of MEI-1 may ensure temporal activation of the katanin complex during meiosis, whereas CRL3(MEL-26)-mediated degradation of both MEI-1 and its activator PPFR-1 ensure efficient katanin inactivation in the transition to mitosis. PMID: 23918937
    4. MEI-1 is required for translocation of the meiosis I spindle to the oocyte cortex. PMID: 12885567
    5. The BTB-containing protein MEL-26 is a component required for degradation of MEI-1 in vivo; importantly, MEL-26 specifically interacts with CUL-3 and MEI-1 in vivo and in vitro, and displays properties of a substrate-specific adaptor PMID: 13679921
    6. the identification of BTB-domain protein MEL-26 and its genetic target MEI-1 as substrate-specific adaptors for C. elegans CUL-3 PMID: 13679922
    7. MEI-1 is the only essential target for MEL-26, and possibly for the E3 ubiquitin ligase CUL-3, but the upstream ubiquitin ligase activating enzyme RFL-1 has additional essential targets PMID: 19361490

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  • 亞細胞定位：
    Cytoplasm, cytoskeleton, spindle pole. Chromosome. Cytoplasm.
  • 蛋白家族：
    AAA ATPase family, Katanin p60 subunit A1 subfamily
  • 數據庫鏈接：

    KEGG: cel:CELE_T01G9.5

    STRING: 6239.T01G9.5b.1

    UniGene: Cel.18411



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