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  4. Recombinant Kribbella flavida Pup--protein ligase (pafA)

Recombinant Kribbella flavida Pup--protein ligase (pafA)

  • 中文名稱：
    Recombinant Kribbella flavida Pup--protein ligase(pafA)
  • 貨號(hào)：
    CSB-YP512168KBS
  • 說明書：
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Kribbella flavida Pup--protein ligase(pafA)
  • 貨號(hào)：
    CSB-EP512168KBS
  • 說明書：
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    Recombinant Kribbella flavida Pup--protein ligase(pafA)
  • 貨號(hào)：
    CSB-EP512168KBS-B
  • 說明書：
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Kribbella flavida Pup--protein ligase(pafA)
  • 貨號(hào)：
    CSB-BP512168KBS
  • 說明書：
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Kribbella flavida Pup--protein ligase(pafA)
  • 貨號(hào)：
    CSB-MP512168KBS
  • 說明書：
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    pafA
  • Uniprot No.：
  • 別名：
    pafA; Kfla_5110; Pup--protein ligase; EC 6.3.1.19; Proteasome accessory factor A; Pup-conjugating enzyme
  • 種屬：
    Kribbella flavida (strain DSM 17836 / JCM 10339 / NBRC 14399)
  • 蛋白長度：
    full length protein
  • 表達(dá)區(qū)域：
    1-453
  • 氨基酸序列
    MNRRIFGLEN EYGVTCTFRG QRRLTPDEVA RYLFRRVVSW GRSSNVFLRN GARLYLDVGS HPEYATGECD SVTDLIAHDK AGERILEGLL VDAQKRLHDE GIFGDVYLFK NNTDSAGNSY GCHENYLVSR HGDFAKLADV LIPFLVTRQL ICGAGKVLQT PRGAVYSVSQ RAEHIWEGVS SATTRSRPII NTRDEPHADA ERFRRLHVIV GDSNMSETTM LLKVASTDLV LRMIEAGIVM RDLTLDNPIR AIREISHDMT GRREVRLANG REASALDIQG EYLSKARDFV DRRELGTPAV ERALSLWERT LKAIESGDLS GIEREIDWVI KYRLIERYRA QNNLGLASPR VAQLDLAYHD IHRPRGLYYL LERKGAVTRV VDDLRVFEAK SVPPQTTRAR LRGEFIKRAQ ERRRDFTVDW VHLKLNDQAQ RTVLCKDPFK SHDERVEKLI ASM
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價(jià)

靶點(diǎn)詳情

  • 功能：
    Catalyzes the covalent attachment of the prokaryotic ubiquitin-like protein modifier Pup to the proteasomal substrate proteins, thereby targeting them for proteasomal degradation. This tagging system is termed pupylation. The ligation reaction involves the side-chain carboxylate of the C-terminal glutamate of Pup and the side-chain amino group of a substrate lysine.
  • 蛋白家族：
    Pup ligase/Pup deamidase family, Pup-conjugating enzyme subfamily
  • 數(shù)據(jù)庫鏈接：


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