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Recombinant Influenza B virus Nucleoprotein (NP)

In Stock
  • 中文名稱:
    Recombinant Influenza B virus Nucleoprotein(NP)
  • 貨號:
    CSB-BP361330IJK
  • 規格:
    ¥3168
  • 圖片:
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他:

產品詳情

  • 純度:
    Greater than 85% as determined by SDS-PAGE.
  • 基因名:
    NP
  • Uniprot No.:
  • 別名:
    NP; Nucleoprotein; Nucleocapsid protein; Protein N
  • 種屬:
    Influenza B virus (strain B/Lee/1940)
  • 蛋白長度:
    Full Length
  • 來源:
    Baculovirus
  • 分子量:
    64.3kDa
  • 表達區域:
    1-560aa
  • 氨基酸序列
    MSNMDIDSINTGTIDKTPEELTPGTSGATRPIIKPATLAPPSNKRTRNPSPERTTTSSETDIGRKIQKKQTPTEIKKSVYKMVVKLGEFYNQMMVKAGLNDDMERNLIQNAQAVERILLAATDDKKTEYQKKRNARDVKEGKEEIDHNKTGGTFYKMVRDDKTIYFSPIKITFLKEEVKTMYKTTMGSDGFSGLNHIMIGHSQMNDVCFQRSKGLKRVGLDPSLISTFAGSTLPRRSGTTGVAIKGGGTLVDEAIRFIGRAMADRGLLRDIKAKTAYEKILLNLKNKCSAPQQKALVDQVIGSRNPGIADIEDLTLLARSMVVVRPSVASKVVLPISIYAKIPQLGFNTEEYSMVGYEAMALYNMATPVSILRMGDDAKDKSQLFFMSCFGAAYEDLRVLSALTGTEFKPRSALKCKGFHVPAKEQVEGMGAALMSIKLQFWAPMTRSGGNEVSGEGGSGQISCSPVFAVERPIALSKQAVRRMLSMNVEGRDADVKGNLLKMMNDSMAKKTSGNAFIGKKMFQISDKNKVNPIEIPIKQTIPNFFFGRDTAEDYDDLDY
    Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
    If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
  • 蛋白標簽:
    N-terminal 10xHis-tagged
  • 產品提供形式:
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液:
    Tris-based buffer,50% glycerol
  • 儲存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    3-7 business days
  • 注意事項:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 產品描述:

    Intact influenza B virus nucleoprotein (NP) cDNA (1-560aa) with an N-terminal 10xHis-tag was expressed in the Baculovirus cells. The forming protein is the recombinant full-length influenza B virus NP protein. The purity of this protein is greater than 85% determined by SDS-PAGE. Under reducing conditions, the gel showed a molecular weight band of about 65 kDa. In-stock influenza B virus NP proteins are offered now. In addition to producing specific antibodies, this recombinant influenza B virus NP protein may be used in the studies of microbiology.

    Nucleoprotein (NP) is the predominant component of the influenza virus ribonucleoprotein. It is highly conserved among each type of influenza viruses. Alice Labaronne etc. demonstrated that the NP of influenza B virus has a long N-terminal tail of 70 residues with intrinsic flexibility. This tail contains the Nuclear Location Signal (NLS). And NP is imported into the nucleus by the importin-α/β pathway via direct interaction with importin-α isoforms. In addition, NP is the potential target for a universal influenza vaccine.

  • Datasheet & COA:
    Please contact us to get it.

產品評價

靶點詳情

  • 功能:
    Encapsidates the negative strand viral RNA, protecting it from nucleases. The encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as template for transcription and replication. The RNP needs to be localized in the host nucleus to start an infectious cycle, but is too large to diffuse through the nuclear pore complex. NP comprises at least 2 nuclear localization signals that are responsible for the active RNP import into the nucleus through cellular importin alpha/beta pathway. Later in the infection, nclear export of RNPs are mediated through viral proteins NEP interacting with M1 which binds nucleoproteins. It is possible that nucleoprotein binds directly host exportin-1/XPO1 and plays an active role in RNPs nuclear export. M1 interaction with RNP seems to hide nucleoprotein's nuclear localization signals. Soon after a virion infects a new cell, M1 dissociates from the RNP under acidification of the virion driven by M2 protein. Dissociation of M1 from RNP unmasks nucleoprotein's nuclear localization signals, targeting the RNP to the nucleus.
  • 基因功能參考文獻:
    1. Results show that the N terminus is involved in the formation of both higher homo-oligomers of BNP and BNP-RNA complex. PMID: 26368391
  • 亞細胞定位:
    Virion. Host nucleus.
  • 蛋白家族:
    Influenza viruses nucleoprotein family
  • 數據庫鏈接:

    KEGG: vg:26824002



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