Recombinant Influenza A virus Nucleoprotein (NP)

^{In Stock}

中文名稱：

Recombinant Influenza A virus Nucleoprotein(NP)
貨號：

CSB-EP302450IGF
規格：

￥2328
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP302450IGF could indicate that this peptide derived from E.coli-expressed Influenza A virus (strain A/Shanghai/16/1989 H3N2) NP.

Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP302450IGF could indicate that this peptide derived from E.coli-expressed Influenza A virus (strain A/Shanghai/16/1989 H3N2) NP.
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靶點詳情

產品詳情

純度：

Greater than 90% as determined by SDS-PAGE.
基因名：

NP
Uniprot No.：

P69296
別名：

NP; Nucleoprotein; Nucleocapsid protein; Protein N
種屬：

Influenza A virus
蛋白長度：

Full Length
來源：

E.coli
分子量：

60.2kDa
表達區域：

1-498aa
氨基酸序列

MASQGTKRSYEQMETDGERQNATEIRASVGKMIDGIGRFYIQMCTELKLSDYEGRLIQNSLTVERMVLSAFDERRNRYLEEHPSAGKDPKKTGGPIYKRVGGRWMRELVLYDKEEIRRIWRQANNGDDATRGLTHMMIWHSNLNDTTYQRTRALVRTGMDPRMCSLMQGSTLPRRSGAAGAAVKGIGTMVMELIRMIKRGINDRNFWRGENGRKTRSAYERMCNILKGKFQTAAQRAMMDQVRESRNPGNAEIEDLIFSARSALILRGSVAHKSCLPACVYGPAVSSGYDFEKEGYSLVGIDPFKLLQNSQVYSLIRPNENPAHKSQLVWMACHSAAFEDLRLLSFIRGTKVSPRGKLSTRGVQIASNENMDNMESSTLELRSRYWAIRTRSGGNTNQQRASAGQISVQPTFSVQRNLPFEKSTVMAAFTGNTEGRTSDMRAEIIRMMEGAKPEEVSFRGRGVFELSDEKATNPIVPSFDMSNEGSYFFGDNAEEYDN
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 6xHis-tagged
產品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
產品描述：

A DNA sequence encoding the 1-498 aa of Influenza A virus was expressed with an N-terminal 6xHis-tag in E.coli. The resulting protein is a recombinant full-length Influenza A virus Nucleoprotein (NP). Its identity was confirmed by the LC-MS/MS Analysis. SDS-PAGE assay determined its purity greater than 90%. This NP protein may be used for specific antibody synthesis or in the studies of the microbiology research area.

Influenza A virus NP is a structural protein with the most abundant expression and no intrinsic enzymatic activity in infected cells. It mainly encapsidates the segmented RNA and then combines with the three polymerase subunits (PA, PB1, and PB2) to form the viral ribonucleoprotein particles (vRNPs) for RNA transcription ＆ replication, and packaging. Various nuclear localization signals (NLS) in the amino acid sequence of NP are responsible for the active RNP import into the nucleus. Besides, NP is one of the main determinants of influenza species specificity. The self-oligomerization of NP is essential for its function.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

Encapsidates the negative strand viral RNA, protecting it from nucleases. The encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as template for transcription and replication. The RNP needs to be localized in the host nucleus to start an infectious cycle, but is too large to diffuse through the nuclear pore complex. NP comprises at least 2 nuclear localization signals that are responsible for the active RNP import into the nucleus through cellular importin alpha/beta pathway. Later in the infection, nclear export of RNPs are mediated through viral proteins NEP interacting with M1 which binds nucleoproteins. It is possible that nucleoprotein binds directly host exportin-1/XPO1 and plays an active role in RNPs nuclear export. M1 interaction with RNP seems to hide nucleoprotein's nuclear localization signals. Soon after a virion infects a new cell, M1 dissociates from the RNP under acidification of the virion driven by M2 protein. Dissociation of M1 from RNP unmasks nucleoprotein's nuclear localization signals, targeting the RNP to the nucleus.
亞細胞定位：

Virion. Host nucleus.
蛋白家族：

Influenza viruses nucleoprotein family