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  4. Recombinant Human herpesvirus 1 Probable ATP-dependent helicase UL5 (UL5), partial

Recombinant Human herpesvirus 1 Probable ATP-dependent helicase UL5 (UL5), partial

  • 中文名稱：
    Recombinant Human herpesvirus 1 Probable ATP-dependent helicase UL5 (UL5) ,partial
  • 貨號：
    CSB-YP318021HWY
  • 說明書：
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Human herpesvirus 1 Probable ATP-dependent helicase UL5 (UL5) ,partial
  • 貨號：
    CSB-EP318021HWY
  • 說明書：
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    Recombinant Human herpesvirus 1 Probable ATP-dependent helicase UL5 (UL5) ,partial
  • 貨號：
    CSB-EP318021HWY-B
  • 說明書：
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Human herpesvirus 1 Probable ATP-dependent helicase UL5 (UL5) ,partial
  • 貨號：
    CSB-BP318021HWY
  • 說明書：
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Human herpesvirus 1 Probable ATP-dependent helicase UL5 (UL5) ,partial
  • 貨號：
    CSB-MP318021HWY
  • 說明書：
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    HELI
  • Uniprot No.：
  • 別名：
    HELI; UL5DNA replication helicase; EC 3.6.4.-
  • 種屬：
    Human herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1)
  • 蛋白長度：
    Partial
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價(jià)

靶點(diǎn)詳情

  • 功能：
    Component of the helicase/primase complex. Unwinds the DNA at the replication forks and generates single-stranded DNA for both leading and lagging strand synthesis. The primase synthesizes short RNA primers on the lagging strand that the polymerase elongates using dNTPs. Possesses helicase-like motifs and therefore may act as the helicase subunit of the complex.
  • 基因功能參考文獻(xiàn)：
    1. Data indicate that the N-terminal domain of UL52 primase binds UL5 helicase and the middle domain interacts with Ul8. PMID: 25278021
    2. Results showed that the UL29 and UL5 transgenes are stable in that there are one and three copies of the UL29 and UL5 genes, respectively, per cell in both the AV529-19 MCB and ECB. PMID: 24140636
    3. HSV-1 single stranded DNA binding protein (ICP8), and the helicase/primase complex (UL8/UL5/UL52) form a nuclear complex in transfected cells that is necessary and sufficient to disable ATR signaling. PMID: 24098119
    4. Taken together, these data suggest that the primase activity of the helicase-primase requires formation of a dimer or higher-order structure while ATPase activity does not. PMID: 21068246
    5. We present evidence that single mutations close to a predicted functional domain of an essential HSV-1 replication enzyme (helicase) are associated with drug resistance and virus growth characteristics. PMID: 17404685
    6. We believe our report to be the first to describe an HPI(helicase-primase inhibitors)-resistant HSV-1 UL5 mutant, that is markedly less virulent in vivo and slowly growing in tissue culture compared to the parental strain. [UL5] PMID: 18539344
    7. Slower growth and moderately reduced virulence suggest that the Asn342Lys mutation might interferes with the helicase-primase activity. PMID: 19403757
    8. Herpes simplex virus-1 DNA primase does not absolutely need to form Watson-Crick hydrogen bonds to efficiently polymerize a NTP. The enzyme can misincorporate nucleotides both by misreading the template and by a primer-template slippage mechanism. PMID: 19835416

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  • 亞細(xì)胞定位：
    Host nucleus.
  • 蛋白家族：
    Herpesviridae helicase family
  • 數(shù)據(jù)庫鏈接：

    KEGG: vg:2703420



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