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  4. Recombinant Human Tubulin monoglycylase TTLL3 (TTLL3 PRO0207)

Recombinant Human Tubulin monoglycylase TTLL3 (TTLL3 PRO0207)

  • 中文名稱：
  • 貨號：
    CSB-YP2332HU
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
  • 貨號：
    CSB-EP2332HU
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
  • 貨號：
    CSB-EP2332HU-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
  • 貨號：
    CSB-BP2332HU
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
  • 貨號：
    CSB-MP2332HU
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    TTLL3
  • Uniprot No.：
  • 別名：
    Tubulin monoglycylase TTLL3; EC 6.3.2.-; HOTTL; Tubulin--tyrosine ligase-like protein 3; TTLL3 PRO0207
  • 種屬：
    Homo sapiens (Human)
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Monoglycylase which modifies alpha- and beta-tubulin, adding a single glycine on the gamma-carboxyl groups of specific glutamate residues to generate monoglycine side chains within the C-terminal tail of tubulin. Not involved in elongation step of the polyglycylation reaction. Preferentially glycylates a beta-tail peptide over the alpha-tail, although shifts its preference toward alpha-tail as beta-tail glutamylation increases. Competes with polyglutamylases for modification site on beta-tubulin substrate, thereby creating an anticorrelation between glycylation and glutamylation reactions. Together with TTLL8, mediates microtubule glycylation of primary and motile cilia, which is essential for their stability and maintenance. Involved in microtubule glycylation of primary cilia in colon which controls cell proliferation of epithelial cells and plays an essential role in colon cancer development. Together with TTLL8, glycylates sperm flagella which regulates axonemal dynein motor activity, thereby controlling flagellar beat, directional sperm swimming and male fertility.
  • 基因功能參考文獻：
    1. Characterization of another human tubulin tyrosine ligase-like gene family member PMID: 11054573
  • 亞細胞定位：
    Cytoplasm, cytoskeleton. Cell projection, cilium. Cytoplasm, cytoskeleton, cilium axoneme. Cytoplasm, cytoskeleton, flagellum axoneme.
  • 組織特異性：
    Expressed in brain, heart, kidney, testis, liver, lung, muscle, spleen, trachea and colon.
  • 數據庫鏈接：

    HGNC: 24483

    KEGG: hsa:26140

    STRING: 9606.ENSP00000392549

    UniGene: Hs.726435



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