Recombinant Human Transcription initiation factor IIE subunit beta (GTF2E2)

中文名稱：

人GTF2E2重組蛋白
貨號：

CSB-EP010003HU
規格：

￥1344
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

Greater than 90% as determined by SDS-PAGE.
基因名：

GTF2E2
Uniprot No.：

P29084
別名：

FE; General transcription factor IIE subunit 2; General transcription factor IIE; polypeptide 2; beta 34kDa ; gtf2e2; T2EB_HUMAN; TF2E 2; TF2E2; TFIIE B; TFIIE-beta; Transcription initiation factor IIE beta subunit; Transcription initiation factor IIE subunit beta
種屬：

Homo sapiens (Human)
蛋白長度：

Full Length
來源：

E.coli
分子量：

37.0kDa
表達區域：

1-291aa
氨基酸序列

MDPSLLRERELFKKRALSTPVVEKRSASSESSSSSSKKKKTKVEHGGSSGSKQNSDHSNGSFNLKALSGSSGYKFGVLAKIVNYMKTRHQRGDTHPLTLDEILDETQHLDIGLKQKQWLMTEALVNNPKIEVIDGKYAFKPKYNVRDKKALLRLLDQHDQRGLGGILLEDIEEALPNSQKAVKALGDQILFVNRPDKKKILFFNDKSCQFSVDEEFQKLWRSVTVDSMDEEKIEEYLKRQGISSMQESGPKKVAPIQRRKKPASQKKRRFKTHNEHLAGVLKDYSDITSSK
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 6xHis-tagged
產品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase.
基因功能參考文獻：
1. functional analysis of Trichothiodystrophy causative TFIIEbeta mutation demonstrates that mutant TFIIEbeta strongly reduces the total amount of the entire TFIIE complex, with a remarkable temperature-sensitive transcription defect. PMID: 28973399
2. We underline role of TFIIE in transcription and its distinction from nucleotide excision repair. Trichothiodystrophy individuals with mutations in GTF2E2 highlight the importance of the direct interaction between TFIIH and TFIIE in transcription process. PMID: 26996949
3. PC4 plays essential roles in the transition step from transcription initiation to elongation by binding to melted DNA in collaboration with TFIIEbeta. PMID: 25308091
4. introduced point mutations into two regions located near the carboxy terminus of TFIIE beta and identified the functionally essential amino acid residues that bind to RNA polymerase II PMID: 12665589
5. TFIIE is an alpha/beta heterodimer in solution; A model for the quaternary architecture of the complex is proposed that provides a structural framework to discuss the function of TFIIE in the context of RNA polymerase II transcription initiation. PMID: 16547462
6. These data suggest a model in which TFIIE binds to a position adjacent to the initiation site via the forkhead domain, enabling TFIIH to begin opening the promoter. PMID: 19210545
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相關疾病：

Trichothiodystrophy 6, non-photosensitive (TTD6)
亞細胞定位：

Nucleus.
蛋白家族：

TFIIE beta subunit family
數據庫鏈接：

HGNC: 4651

OMIM: 189964

KEGG: hsa:2961

STRING: 9606.ENSP00000348168

UniGene: Hs.77100