在线日韩日本国产亚洲丨少妇伦子伦情品无吗丨欧美性猛交xxxx免费看蜜桃丨精品人妻系列无码一区二区三区丨亚洲精品无码不卡在线播放

Your Good Partner in Biology Research

Recombinant Human Serine/threonine-protein kinase PLK2 (PLK2)

  • 中文名稱:
    人PLK2重組蛋白
  • 貨號:
    CSB-EP885770HU
  • 規格:
    ¥1536
  • 圖片:
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他:

產品詳情

  • 純度:
    Greater than 90% as determined by SDS-PAGE.
  • 生物活性:
    Not Test
  • 基因名:
  • Uniprot No.:
  • 別名:
    (Polo-like kinase 2)(PLK-2)(hPlk2)(Serine/threonine-protein kinase SNK)(hSNK)(Serum-inducible kinase)
  • 種屬:
    Homo sapiens (Human)
  • 蛋白長度:
    Full Length
  • 來源:
    E.coli
  • 分子量:
    85.8
  • 表達區域:
    1-685aa
  • 氨基酸序列
    MELLRTITYQPAASTKMCEQALGKGCGADSKKKRPPQPPEESQPPQSQAQVPPAAPHHHHHHSHSGPEISRIIVDPTTGKRYCRGKVLGKGGFAKCYEMTDLTNNKVYAAKIIPHSRVAKPHQREKIDKEIELHRILHHKHVVQFYHYFEDKENIYILLEYCSRRSMAHILKARKVLTEPEVRYYLRQIVSGLKYLHEQEILHRDLKLGNFFINEAMELKVGDFGLAARLEPLEHRRRTICGTPNYLSPEVLNKQGHGCESDIWALGCVMYTMLLGRPPFETTNLKETYRCIREARYTMPSSLLAPAKHLIASMLSKNPEDRPSLDDIIRHDFFLQGFTPDRLSSSCCHTVPDFHLSSPAKNFFKKAAAALFGGKKDKARYIDTHNRVSKEDEDIYKLRHDLKKTSITQQPSKHRTDEELQPPTTTVARSGTPAVENKQQIGDAIRMIVRGTLGSCSSSSECLEDSTMGSVADTVARVLRGCLENMPEADCIPKEQLSTSFQWVTKWVDYSNKYGFGYQLSDHTVGVLFNNGAHMSLLPDKKTVHYYAELGQCSVFPATDAPEQFISQVTVLKYFSHYMEENLMDGGDLPSVTDIRRPRLYLLQWLKSDKALMMLFNDGTFQVNFYHDHTKIIICSQNEEYLLTYINEDRISTTFRLTTLLMSGCSSELKNRMEYALNMLLQRCN
    Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
    If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
  • 蛋白標簽:
    N-terminal 10xHis-tagged and C-terminal Myc-tagged
  • 產品提供形式:
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液:
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 復溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 注意事項:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
  • Datasheet & COA:
    Please contact us to get it.

產品評價

靶點詳情

  • 功能:
    Tumor suppressor serine/threonine-protein kinase involved in synaptic plasticity, centriole duplication and G1/S phase transition. Polo-like kinases act by binding and phosphorylating proteins are that already phosphorylated on a specific motif recognized by the POLO box domains. Phosphorylates CENPJ, NPM1, RAPGEF2, RASGRF1, SNCA, SIPA1L1 and SYNGAP1. Plays a key role in synaptic plasticity and memory by regulating the Ras and Rap protein signaling: required for overactivity-dependent spine remodeling by phosphorylating the Ras activator RASGRF1 and the Rap inhibitor SIPA1L1 leading to their degradation by the proteasome. Conversely, phosphorylates the Rap activator RAPGEF2 and the Ras inhibitor SYNGAP1, promoting their activity. Also regulates synaptic plasticity independently of kinase activity, via its interaction with NSF that disrupts the interaction between NSF and the GRIA2 subunit of AMPARs, leading to a rapid rundown of AMPAR-mediated current that occludes long term depression. Required for procentriole formation and centriole duplication by phosphorylating CENPJ and NPM1, respectively. Its induction by p53/TP53 suggests that it may participate in the mitotic checkpoint following stress.
  • 基因功能參考文獻:
    1. The p53 dependence of Plk2 loss and tumor suppressor function in relationship to mTOR signaling may have therapeutic implications. PMID: 29448085
    2. FOXD1 promoted the expression of Plk2 mRNA and protein in colorectal cancer cells. PMID: 29864920
    3. we discovered that PLK2 might be inactivated in microsatellite-instability high colorectal cancers, suggesting that PLK2 could be a tumor-suppressing gene in these cancers. PMID: 28698103
    4. study demonstrates that PLK-2 activity can rapidly change cellular alpha-synuclein levels in cell models and in mice brains, but this process does not require phosphorylation of S129. Instead, it operates via regulation of alpha-synuclein mRNA transcription in an open reading frame-dependent manner PMID: 28648742
    5. Findings indicate the interaction network of viral oncogene HPV16 E7, miR-27b and PLK2, and support the potential strategies using antisense nucleic acid of miR-27b for therapy of cervical cancer. PMID: 26910911
    6. CHOP negatively regulates Polo-like kinase 2 expression via recruiting C/EBPalpha to the upstream-promoter in human osteosarcoma cell line during ER stress PMID: 28652211
    7. Plk2 represents an independent prognostic marker and regulates tumor growth and apoptosis by targeting Fbxw7/Cyclin E pathway in colorectal carcinoma. PMID: 27423313
    8. PLK2 kinase has a role in mediating alpha-synuclein selective autophagic degradation PMID: 28154193
    9. PLK2, functional kinase and polo-box domains were required for interaction with PFV Gag. Fluorescently-tagged PFV Gag, through its chromatin tethering function, selectively relocalized ectopically expressed eGFP-tagged PLK proteins to mitotic chromosomes i PMID: 27579920
    10. Develop a DDS for continuous intracellular delivery of PLK2. PMID: 27863657
    11. Structural analysis of the polo-box domain of human PLK2 has been presented. PMID: 25846005
    12. our study demonstrates a novel mechanism of PLK2 in promoting tumor progression, whereby it directly binds to enriched TAp73, catalyzes Ser48 phosphorylation of TAp73, and inhibits TAp73 transcriptional activity PMID: 26625870
    13. Data suggest wild-type SNCA (alpha-synuclein) binding to synaptosome membrane is not affected by phosphorylation by PLK2; A30P SNCA, a Parkinson disease mutation, binding is greatly increased; endocytosis of SNCA fibrils follows similar pattern. PMID: 26719332
    14. Decreased PLK2 protein expression due to promoter hypermethylation was negatively correlated with JAK2 overexpression, a common occurrence in hematological malignancies. PMID: 25347426
    15. PLK2 SNPs were associated with Alzheimer disease and mild cognitive impairment. PLK2-rs15009 CC and GG genotypes and CC genotype at PLK2-rs702723 were protective for AD. PMID: 24384746
    16. PLK2 indirectly activates ROCK2 via phosphorylating nucleophosmin during centrosome amplification. PMID: 25590559
    17. these findings reveal a conserved PLK2-RAP1 pathway that is crucial to regulate endothelial tip cell behavior in order to ensure proper vascular development and patterning in vertebrates. PMID: 26004360
    18. Silencing of polo-like kinase 2 increases cell proliferation and decreases apoptosis in SGC-7901 gastric cancer cells. PMID: 25501818
    19. Unique PLK2-dependent protein phosphorylation sites identified by mass spectrometry. PMID: 25338102
    20. the regulation of redox homeostasis by PLK2 promotes the survival of cells with dysfunctional mitochondria PMID: 24887096
    21. Data shows that PLK2 mRNA is a direct target of miR-27a in laryngeal squamous cell carcinoma. PMID: 25239093
    22. this study reports the first crystal structure of the C-terminal polo-box domain of PLK2. PMID: 25511705
    23. Study indicates that miR-126 is a tumor suppressor that inhibits gastric cancer cells proliferation by targeting PI3KR2, Crk and PLK2. PMID: 24969300
    24. This study supports a role for PLK2 in the generation of aSyn inclusions by a mechanism that does not depend directly on serine 129 phosphorylation. PMID: 23677647
    25. Our findings suggest a new role for PLK2 in the regulation of inflammatory diseases by modulating ADAM17 activity. PMID: 24338472
    26. Polo-like kinase 2 is a mediator of hedgehog survival signaling in cholangiocarcinoma. PMID: 23703673
    27. PLK2 inhibition is a tractable CNS pharmacological target that does not cause genotoxicity at doses and exposures that engage the target in the sensory retina. PMID: 23466428
    28. Phosphorylation of Fbxw7 by Plk2 induces destabilization of the F-box protein resulting in accumulation of cyclin E and increased potential for centriole reproduction PMID: 22399798
    29. have identified and validated as in vitro PLK2 and PLK3 substrates HSP90, GRP-94, beta-tubulin, calumenin, and 14-3-3 epsilon PMID: 22828320
    30. Mutant p53 oncogenic functions are sustained by Plk2 kinase through an autoregulatory feedback loop. PMID: 22134238
    31. The methylation status of the PLK2 CpG island predicts outcomes in patients treated with carboplatin and paclitaxel chemotherapy. PMID: 22289679
    32. phosphorylation sites could play an important role in the rapid activation, expansion, and prolongation of Plk2 signaling PMID: 22100274
    33. PLK2 and to a lesser extent PLK3 are superior over CK2, as catalysts of Ser-129 phosphorylation both in full length alpha-synuclein and in a peptide reproducing the C-terminal segment of the protein. PMID: 22248692
    34. Acetylation of GATA-1 responsive elements in Polo-like kinase 2 plays a crucial role in Plk2 transcription and protein expression in MG-63 cells. PMID: 22138223
    35. Polo-like kinase 2 (SNK/PLK2) is a novel epigenetically regulated gene in acute myeloid leukemia and myelodysplastic syndromes. PMID: 21340720
    36. Results implicate Plk2 as a clinically important determinant of chemosensitivity. PMID: 21402713
    37. Identification of Snk/Plk2 as a novel methylated gene in multiple myeloma. PMID: 21067440
    38. NPM/B23 is a direct target of Plk2 in the regulation of centriole duplication and that phosphorylation on serine 4 can trigger this process PMID: 20352051
    39. Results identify centrosomal P4.1-associated protein (CPAP), a human homologue of SAS-4, as a substrate of PLK2 whose activity oscillates during the cell cycle. PMID: 20531387
    40. overexpression of PLK2 in bladder carcinomas, suggesting a possible role of PLK2 in the pathogenesis of bladder carcinomas PMID: 19506885
    41. Our results point to a novel Plk2-TSC1 interaction with effects on mTOR signaling during hypoxia, and tumor growth that may enable targeting Plk2 signaling in cancer therapy. PMID: 20054236
    42. there is a mitotic checkpoint wherein p53-dependent activation of Snk/Plk2 prevents mitotic catastrophe following spindle damage PMID: 12897130
    43. hVPS18 may play an important role in regulation of SNK activity through its ubiquitin ligase PMID: 16203730
    44. Calcipotriol affects proliferation of keratinocytes by decreasing the expression of EGR1 and PLK2 PMID: 17671831
    45. is a novel target of ERRgamma, and their interaction is crucial for cancer cell proliferation PMID: 17706602
    46. increased cell death observed after aphidicolin treatment and release in Plk2-deficient cells may result from both higher levels of replication stress-induced DNA damage and a dysfunctional S-phase checkpoint PMID: 17912033
    47. PLK2 directly phosphorylates alpha-synuclein at Ser-129 in an in vitro biochemical assay PMID: 19004816
    48. Data show that PLK2 as a kinase that phosphorylates Ser-137 of PLK1, which is sufficient to mediate this survival signal. PMID: 19706541

    顯示更多

    收起更多

  • 亞細胞定位:
    Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole. Cell projection, dendrite. Note=Localizes to centrosomes during early G1 phase where it only associates to the mother centriole and then distributes equally to both mother and daughter centrioles at the onset of S phase.
  • 蛋白家族:
    Protein kinase superfamily, Ser/Thr protein kinase family, CDC5/Polo subfamily
  • 組織特異性:
    Expressed at higher level in the fetal lung, kidney, spleen and heart.
  • 數據庫鏈接:

    HGNC: 19699

    OMIM: 607023

    KEGG: hsa:10769

    STRING: 9606.ENSP00000274289

    UniGene: Hs.398157



主站蜘蛛池模板: 日本伊人色综合网| 亚洲精品久久婷婷丁香51| 亚洲成a人v欧美综合天堂麻豆| 日本大片免a费观看视频三区| 无码熟熟妇丰满人妻啪啪| 日本一卡2卡3卡4卡5卡精品视频| 国产成人无码午夜视频在线播放| 少妇把腿扒开让我舔18| 中文字幕一区二区人妻电影| 丰满少妇高潮惨叫久久久一| 久久久av男人的天堂| 亚洲精品综合一区二区| 玩弄丰满少妇xxxxx性多毛| 亚洲国产长腿丝袜av天堂| 无码区日韩特区永久免费系列| 97久章草在线视频播放| 国产av成人无码精品网站| 一边吃奶一边做动态图| 亚洲精品自在在线观看| 国产在线观看无码的免费网站| 男插女高潮一区二区| 亚洲综合色88综合天堂| 成人午夜福利视频镇东影视| 欧牲交a欧美牲交aⅴ| 免费做a爰片久久毛片a片下载| 精品人妻无码一区二区三区蜜桃一| 疯狂撞击丝袜人妻| 国产精品女上位好爽在线| 2021精品亚洲中文字幕| 国产在线视频www色| 又大又黄又粗又爽的免费视频| 越猛烈欧美xx00动态图| √最新版天堂资源网在线下载| 免费人成视频x8x8| 久久超碰精品一夜七次郎| 久久久久久久久久久免费精品| 免费看欧美成人a片无码| 国产高清av在线播放| 人妻护士在线波多野结衣| 丰满多毛少妇做爰视频爽爽和| 成人免费区一区二区三区|