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  4. Recombinant Human RNA polymerase II subunit A C-terminal domain phosphatase SSU72 (SSU72)

Recombinant Human RNA polymerase II subunit A C-terminal domain phosphatase SSU72 (SSU72)

  • 中文名稱：
    人SSU72重組蛋白
  • 貨號：
    CSB-YP865091HU
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    人SSU72重組蛋白
  • 貨號：
    CSB-EP865091HU
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    人SSU72重組蛋白
  • 貨號：
    CSB-EP865091HU-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    人SSU72重組蛋白
  • 貨號：
    CSB-BP865091HU
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    人SSU72重組蛋白
  • 貨號：
    CSB-MP865091HU
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    SSU72
  • Uniprot No.：
  • 別名：
    CTD phosphatase SSU72; HSPC182; Hypothetical protein FLJ10947; PNAS-120; RNA polymerase II subunit A C-terminal domain phosphatase SSU72; ssu72; Ssu72 RNA polymerase II CTD phosphatase homolog; Ssu72 RNA polymerase II CTD phosphatase homolog (yeast); SSU72_HUMAN
  • 種屬：
    Homo sapiens (Human)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-194
  • 氨基酸序列
    MPSSPLRVAV VCSSNQNRSM EAHNILSKRG FSVRSFGTGT HVKLPGPAPD KPNVYDFKTT YDQMYNDLLR KDKELYTQNG ILHMLDRNKR IKPRPERFQN CKDLFDLILT CEERVYDQVV EDLNSREQET CQPVHVVNVD IQDNHEEATL GAFLICELCQ CIQHTEDMEN EIDELLQEFE EKSGRTFLHT VCFY
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Protein phosphatase that catalyzes the dephosphorylation of the C-terminal domain of RNA polymerase II. Plays a role in RNA processing and termination. Plays a role in pre-mRNA polyadenylation via its interaction with SYMPK.
  • 基因功能參考文獻：
    1. a hand-off model in which Ssu72 mediates a conformational transition in TFIIB, accounting for the role of Ssu72 in transcription reinitiation, gene looping, and promoter-terminator cross-talk. PMID: 29158257
    2. Aurora B kinase directly targets Ssu72 phosphatase for regulation of sister chromatid cohesion during early mitosis. PMID: 24149858
    3. The pSer7 phosphatase activity of Ssu72 is approximately 4000-fold lower than its pSer5 phosphatase activity toward a peptide substrate PMID: 23070812
    4. Data suggest that Ssu72 is a novel cohesin-binding protein capable of regulating cohesion between sister chromatid arms. PMID: 20818333
    5. crystal structure at 2.4 A resolution of the amino-terminal domain (residues 30-340) of human symplekin in a ternary complex with the Pol II carboxy-terminal domain (CTD) Ser 5 phosphatase Ssu72 and a CTD Ser 5 phosphopeptide PMID: 20861839
    6. Molecular cloning and characterization of functions of human ssu72. PMID: 15659578

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  • 亞細胞定位：
    Nucleus. Cytoplasm. Note=Predominantly in the cytosol.
  • 蛋白家族：
    SSU72 phosphatase family
  • 數據庫鏈接：

    HGNC: 25016

    OMIM: 617680

    KEGG: hsa:29101

    STRING: 9606.ENSP00000291386

    UniGene: Hs.30026



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