Recombinant Human Protein phosphatase 1 regulatory subunit 15A (PPP1R15A), partial

中文名稱：

人PPP1R15A重組蛋白
貨號：

CSB-YP018528HU
規格：

￥2208
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

Greater than 90% as determined by SDS-PAGE.
基因名：

PPP1R15A
Uniprot No.：

O75807
別名：

Apoptosis associated protein; GADD 34; Growth arrest and DNA damage inducible 34; Growth arrest and DNA damage-inducible protein GADD34; Myeloid differentiation primary response protein MyD116 homolog; Ppp1r15a; PR15A_HUMAN; Protein phosphatase 1 regulatory (inhibitor) subunit 15A; Protein phosphatase 1 regulatory subunit 15A
種屬：

Homo sapiens (Human)
蛋白長度：

Partial
來源：

Yeast
分子量：

71.1kDa
表達區域：

40-674aa
氨基酸序列

GPLEPWLVEAVKGAALVEAGLEGEARTPLAIPHTPWGRRPEEEAEDSGGPGEDRETLGLKTSSSLPEAWGLLDDDDGMYGEREATSVPRGQGSQFADGQRAPLSPSLLIRTLQGSDKNPGEEKAEEEGVAEEEGVNKFSYPPSHRECCPAVEEEDDEEAVKKEAHRTSTSALSPGSKPSTWVSCPGEEENQATEDKRTERSKGARKTSVSPRSSGSDPRSWEYRSGEASEEKEEKAHKETGKGEAAPGPQSSAPAQRPQLKSWWCQPSDEEEGEVKALGAAEKDGEAECPPCIPPPSAFLKAWVYWPGEDTEEEEDEEEDEDSDSGSDEEEGEAEASSSTPATGVFLKSWVYQPGEDTEEEEDEDSDTGSAEDEREAETSASTPPASAFLKAWVYRPGEDTEEEEDEDVDSEDKEDDSEAALGEAESDPHPSHPDQRAHFRGWGYRPGKETEEEEAAEDWGEAEPCPFRVAIYVPGEKPPPPWAPPRLPLRLQRRLKRPETPTHDPDPETPLKARKVRFSEKVTVHFLAVWAGPAQAARQGPWEQLARDRSRFARRITQAQEELSPCLTPAARARAWARLRNPPLAPIPALTQTLPSSSVPSSPVQTTPLSQAVATPSRSSAAAAAALDLSGRRG
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 6xHis-tagged
產品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
產品描述：

An ORF cDNA corresponding to the peptide of human protein phosphatase 1 regulatory subunit 15A (PPP1R15A) was expressed with an N-terminal 6xHis-tag in the yeast cells. PPP1R15A CSB-YP018528HU is a truncated molecule having amino acids of Gly40-Gly674. Its purity is greater than 90% measured by SDS-PAGE. A molecular mass band of about 75 kDa was presented on the SDS-PAGE gel under reducing conditions. This recombinant PPP1R15A protein may find uses in the specific antibody synthesis or the studies of epigenetics and nuclear signal transduction.

PPP1R15A, also called GADD34, is transcriptionally induced by stress, downstream of cAMP‐dependent transcription factor ATF4, and C/EBP homologous protein. Its translation escapes the general attenuation of protein synthesis resulting from eIF2α phosphorylation. The stress‐induced eIF2α holohosphatase promotes dephosphorylation of eIF2α in stressed cells, thereby allowing protein synthesis to resume. Thus, PPP1R15A‐PP1 is a crucial effector of the negative feedback loop that shifts cells away from stress to basal activity.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

Recruits the serine/threonine-protein phosphatase PP1 to dephosphorylate the translation initiation factor eIF-2A/EIF2S1, thereby reversing the shut-off of protein synthesis initiated by stress-inducible kinases and facilitating recovery of cells from stress. Down-regulates the TGF-beta signaling pathway by promoting dephosphorylation of TGFB1 by PP1. May promote apoptosis by inducing TP53 phosphorylation on 'Ser-15'.
基因功能參考文獻：
1. Loss of GADD34 cause vanishing white matter disease due to translation defects. PMID: 29632131
2. Compared with normal controls, the wild type TT and allele T of rs611251 of PPP1R15A showed higher frequency in gastric carcinoma, nasopharyngeal carcinomas and lymphomas. PMID: 29186961
3. Inhibition of IRE1 modifies the hypoxic regulation of GADD34 family gene expression in cultured glioma cells. PMID: 29227599
4. GADD34 constitutes a mechanistic link between endoplasmic reticulum stress and mTOR inactivation, therefore promotes cell survival during endoplasmic reticulum stress. PMID: 27992581
5. reduction of GADD34 expression significantly suppressed tumor, and resulted in decreased accumulation of MDSCs and T-cells, and inhibition of GADD34 reduced secretion of vascular epithelial growth factor alpha and transforming growth factor beta by MDSCs PMID: 27630304
6. ANXA11 rs1049550 and PPP1R15A rs557806 may improve the identification of mCRC patients sensitive to bevacizumab regimens, and further validation is required in large cohorts PMID: 27177629
7. Data of this study strengthen the evidence of an unfolded protein response during the course of RA and provide an insight of the potential interest in GADD34 as a relevant marker for RA. PMID: 26829377
8. The results suggest that dephosphorylation of eIF2a by GADD34 plays an important role in doxorubicin resistance of MCF-7/ADR cells. PMID: 26743901
9. The reactive oxygen species-generating NADPH oxidase-4 (Nox4) is induced downstream of ATF4, binds to a PP1-targeting subunit GADD34 at the endoplasmic reticulum, and inhibits PP1 activity to increase eIF2alpha phosphorylation and ATF4 levels. PMID: 26742780
10. stress pathways lead to the induction of the protein GADD34, which appears to provide protection against the toxic effects of the secreted virulence factors in Pseudomonas aeruginosa infection PMID: 26083346
11. The data highlight independent interactions of PP1 and eIF2alpha with GADD34, demonstrating that GADD34 functions as a scaffold both in vitro and in cells PMID: 26095357
12. GADD34 may play a neuroprotective role against amyloid-beta toxicity. PMID: 25204313
13. GADD34 enhances autophagy and suppresses apoptosis stimulated by LPS combined with amino acid deprivation through regulation of mTOR signaling pathway in macrophages. PMID: 25659802
14. Data indicate that protein phosphatase 1 subunit GADD34 directly interacts with eukaryotic translation initiation factor 2 subunit alpha (eIF2alpha). PMID: 26100893
15. GADD34 was increased in neurons of human Alzheimer's disease (AD) brains. Additionally, this finding was also observed in oligodendrocytes in human AD brains. GADD34 could be a therapeutic target for preventing ER stress in neuronal cells in AD. PMID: 26142647
16. GADD34 promotes cell survival and adaptation to increased extracellular osmolarity by increasing the uptake of small neutral amino acids via the amino acid transporter SNAT2. PMID: 26041779
17. Nuclear export of HTLV-1 basic leucine-zipper factor (HBZ) is essential for its interaction with GADD34 and increased phosphorylation of S6 kinase, which is an established downstream target of the mTOR pathway. PMID: 23708656
18. GADD34 phosphorylation on tyrosine 262 modulates endoplasmic reticulum stress signaling and cell fate. PMID: 24092754
19. Low GADD34 expression is associated with malignant mesothelioma. PMID: 23412101
20. the association of with ER modulates intracellular trafficking and proteasomal degradation of GADD34, and in turn, its ability to modify ER morphology PMID: 21518769
21. The data suggest that EBNA3C interacts with Gadd34, activating the upstream component of the UPR-unfolded protein response (eIF2alpha phosphorylation) while preventing downstream UPR events (XBP1 activation and ATF6 cleavage). PMID: 20040105
22. The role of the PP1/GADD34 complex in the molecular cascade is to the translocation of CRT to the outer leaflet of the plasma membrane. PMID: 19901557
23. SNF5/INI1 protein facilitates the function of the growth arrest and DNA damage-inducible protein and modulated protein phosphatase-1 activity PMID: 12016208
24. GADD 34 may play an important role in melanoma progression PMID: 12168790
25. the GADD34-mediated cellular stress response is suppressed by BAG-1 PMID: 12724406
26. Human Gadd34 lacking the viral homology domain does not interfere with normal Gadd34-induced apoptosis in cultured cells. This suggests that viral similarity sequences may be required for Gadd34-mediated functions other than apoptosis. PMID: 12813455
27. These findings suggest that phenethylisothiocyanate creates an oxidative cellular environment that induces DNA damage and GADD153, 34 and 45 gene activation, which in turn helps trigger apoptosis PMID: 14635187
28. GADD34-PP1c recruited by Smad7 inhibits TGFbeta-induced cell cycle arrest. PMID: 14718519
29. the up-regulation of GADD34 in response to global ischaemia in the human brain plus its influence on protein synthesis and DNA repair suggests that this protein may have the potential to influence cell survival PMID: 15541008
30. GADD34 may perform important functions in cardiac tissue in response to ischaemia. PMID: 16337513
31. During conditions of cell stress, GADD34 forms a stable complex with tuberous sclerosis complex (TSC) 1/2, causes TSC2 dephosphorylation, and inhibits signaling by mammalian target of the rapamycin (mTOR). PMID: 17273797
32. mechanisms that control GADD34 levels in human cells PMID: 18794359
33. GADD34 translation is regulated by a unique 5'UTR uORF mechanism to ensure proper GADD34 expression during eIF2alpha phosphorylation PMID: 19131336
34. Infectious bronchitis virus has developed a combination of two mechanisms, i.e., blocking PKR activation and inducing GADD34 expression, to maintain de novo protein synthesis in IBV-infected cells and, meanwhile, to enhance viral replication. PMID: 19776135
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亞細胞定位：

Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side. Mitochondrion outer membrane; Peripheral membrane protein; Cytoplasmic side.
蛋白家族：

PPP1R15 family
數據庫鏈接：

HGNC: 14375

OMIM: 611048

KEGG: hsa:23645

STRING: 9606.ENSP00000200453

UniGene: Hs.631593