Recombinant Human Protein chibby homolog 1 (CBY1)

中文名稱：

Recombinant Human Protein chibby homolog 1(CBY1)
貨號：

CSB-EP896889HU
規格：

￥1344
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

Greater than 90% as determined by SDS-PAGE.
基因名：

CBY1
Uniprot No.：

Q9Y3M2
別名：

ARPP-binding protein; C22orf2; CBY; Cby1; CBY1_HUMAN; Cytosolic leucine rich protein ; Cytosolic leucine-rich protein; Golgi and endoplasmic reticulum-associated 1; HRIHFB2025; PIGEA 14; PIGEA-14; PKD2 interactor; PKD2 interactor, Golgi and endoplasmic reticulum-associated 1; Protein Chibby; Protein chibby homolog 1
種屬：

Homo sapiens (Human)
蛋白長度：

Full Length
來源：

E.coli
分子量：

41.5kDa
表達區域：

1-126aa
氨基酸序列

MPFFGNTFSPKKTPPRKSASLSNLHSLDRSTREVELGLEYGSPTMNLAGQSLKFENGQWIAETGVSGGVDRREVQRLRRRNQQLEEENNLLRLKVDILLDMLSESTAESHLMEKELDELRISRKRK
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal GST-tagged
產品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

Inhibits the Wnt/Wingless pathway by binding to CTNNB1/beta-catenin and inhibiting beta-catenin-mediated transcriptional activation through competition with TCF/LEF transcription factors. Has also been shown to play a role in regulating the intracellular trafficking of polycystin-2/PKD2 and possibly of other intracellular proteins. Promotes adipocyte and cardiomyocyte differentiation.
基因功能參考文獻：
1. FAM92 proteins interact with Cby1 to promote ciliogenesis via regulation of membrane-remodeling processes. PMID: 27528616
2. chronic Cby1 knockdown in colon cancer cells may counteract tumor progression by promoting the mesenchymal-to-epithelial transition process. PMID: 28107095
3. Cby's C-terminal domain alone binds to TC-1 with significantly greater affinity compared to full-length Cby, implying that target binding of the coiled-coil domain is affected by the flanking disordered regions. PMID: 27082063
4. CBY1 downregulation in CML comes from reduced protein stability when bound to 14-3-3sigma adapter protein. Dissociation raises CBY1 protein levels by enhancing its stability. The ubiquitin proteasome system reduces 14-3-3sigma-bound CBY1 stability by SUMOylation. PMID: 26147002
5. Study showed that expression of Cby protein was strongly downregulated in laryngeal squamous cell carcinoma (LSCC) tumor tissues in comparison to normal laryngeal mucosa samples. PMID: 25175341
6. the impact of BCR-ABL1 on Chibby1, a beta catenin antagonist involved in cell differentiation and transformation PMID: 24339928
7. Stable expression of CBY1 drives beta catenin cytoplasmatic translocation and impairs beta catenin signaling in BCR-ABL + cells. PMID: 23707389
8. Results from the association analysis suggest that common variation in CBY is not a cause for obesity in the Belgian population. PMID: 23645032
9. C-terminal polycystin-2 influences the interaction with PIGEA14 PMID: 23838289
10. Cby plays an important role in organization of both primary and motile cilia in collaboration with Cnx. PMID: 22911743
11. The N-terminal portion of the CBY1 is unstructured in solution, but the C-terminal half forms a coiled-coil structure. PMID: 21182262
12. Chibby and clusterin were co-immunoprecipitated with NBPF1. PMID: 20096688
13. These findings unravel the molecular basis through which a combinatorial action of Cby and 14-3-3 proteins controls the dynamic nuclear-cytoplasmic trafficking of beta-catenin. PMID: 19940019
14. Chibby inhibits beta-catenin-mediated transcriptional activation by competing with Lef-1 to bind to beta-catenin PMID: 12712206
15. PIGEA-14 plays an important role in regulating the intracellular location of polycystin-2 PMID: 15194699
16. As no somatic mutation was detected in C22orf2 in 36 colorectal tumour DNA, our results do not support the implication of Chibby as a tumour suppressor in colorectal carcinogenesis. PMID: 15245581
17. Chibby is not likely to promote colorectal carcinoma tumor development or progression. PMID: 16570344
18. CBY promotes adipocyte differentiation through inhibition of beta catenin signaling. PMID: 17403895
19. intrinsically disordered TC-1 interacts with Cby via its transient helical structure PMID: 17905836
20. 14-3-3epsilon and 14-3-3zeta are identified as Cby-binding partners. PMID: 18573912
21. genes including CHIBBY involved in pediatric ependymomas. PMID: 18663750
22. Alanine substitutions of two or more of four critical leucine residues within the C-terminal heptad repeats eliminate the Cby-Cby interaction. PMID: 19435523
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亞細胞定位：

Nucleus speckle. Cytoplasm, cytoskeleton, cilium basal body. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole. Golgi apparatus. Golgi apparatus, trans-Golgi network.
蛋白家族：

Chibby family
組織特異性：

Widely expressed. Expressed at higher levels in heart, skeletal muscle, kidney and placenta. Also found in brain, lung, liver and testis. Significantly down-regulated in thyroid and metastatic uterine tumors.
數據庫鏈接：

HGNC: 1307

OMIM: 607757

KEGG: hsa:25776

STRING: 9606.ENSP00000216029

UniGene: Hs.334911