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  4. Recombinant Human Protein Mis18-alpha (MIS18A)

Recombinant Human Protein Mis18-alpha (MIS18A)

  • 中文名稱：
    人MIS18A重組蛋白
  • 貨號(hào)：
    CSB-YP885766HU
  • 說明書：
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    人MIS18A重組蛋白
  • 貨號(hào)：
    CSB-EP885766HU
  • 說明書：
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    人MIS18A重組蛋白
  • 貨號(hào)：
    CSB-EP885766HU-B
  • 說明書：
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    人MIS18A重組蛋白
  • 貨號(hào)：
    CSB-BP885766HU
  • 說明書：
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    人MIS18A重組蛋白
  • 貨號(hào)：
    CSB-MP885766HU
  • 說明書：
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    MIS18A
  • Uniprot No.：
  • 別名：
    MIS18A; C21orf45; C21orf46; FASP1; Protein Mis18-alpha; FAPP1-associated protein 1
  • 種屬：
    Homo sapiens (Human)
  • 蛋白長(zhǎng)度：
    full length protein
  • 表達(dá)區(qū)域：
    1-233
  • 氨基酸序列
    MAGVRSLRCS RGCAGGCECG DKGKCSDSSL LGKRLSEDSS RHQLLQKWAS MWSSMSEDAS VADMERAQLE EEAAAAEERP LVFLCSGCRR PLGDSLSWVA SQEDTNCILL RCVSCNVSVD KEQKLSKREK ENGCVLETLC CAGCSLNLGY VYRCTPKNLD YKRDLFCLSV EAIESYVLGS SEKQIVSEDK ELFNLESRVE IEKSLTQMED VLKALQMKLW EAESKLSFAT CKS
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Required for recruitment of CENPA to centromeres and normal chromosome segregation during mitosis.
  • 基因功能參考文獻(xiàn)：
    1. The authors demonstrate that CDK1 controls Mis18 complex recruitment to centromeres by regulating oligomerization of M18BP1 through the Mis18alpha:Mis18beta scaffold. PMID: 28059702
    2. the Mis18 complex contains dual CENP-C recognition motifs that are combinatorially required to generate robust centromeric localization that leads to CENP-A deposition PMID: 27239045
    3. Mis18alpha is important for epidermal cell proliferation and stratification, because it is required for the deposition of CENP-A at the centromeric nucleosomes. PMID: 27670610
    4. Stable binding of Mis18alpha-Mis18beta heterotetramer to centromeres in telophase licenses them for CENP-A deposition. PMID: 26942680
    5. it is suggested that pp5644 physically bind to FASP1 PMID: 15881666
    6. Three human proteins essential for centromere/kinetochore structure and function, hMis18alpha, hMis18beta, and M18BP1, the complex of which is accumulated specifically at the telophase-G1 centromere, are identified.[Mis18alpha, Mis18beta, M18BP1] PMID: 17199038

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  • 亞細(xì)胞定位：
    Nucleus. Chromosome. Chromosome, centromere. Note=Associated with centromeres in interphase cells, from late anaphase to the G1 phase. Not detected on centromeres during earlier phases of mitosis. Associated with chromatin.
  • 蛋白家族：
    Mis18 family
  • 組織特異性：
    Detected in testis.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 1286

    KEGG: hsa:54069

    STRING: 9606.ENSP00000290130

    UniGene: Hs.190518



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