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  4. Recombinant Human Potential tRNA (adenine (58)-N (1))-methyltransferase catalytic subunit TRMT61B (TRMT61B)

Recombinant Human Potential tRNA (adenine (58)-N (1))-methyltransferase catalytic subunit TRMT61B (TRMT61B)

  • 中文名稱：
    人TRMT61B重組蛋白
  • 貨號(hào)：
    CSB-YP863133HU
  • 說(shuō)明書：
  • 規(guī)格：
  • 來(lái)源：
    Yeast
  • 其他：
  • 中文名稱：
    人TRMT61B重組蛋白
  • 貨號(hào)：
    CSB-EP863133HU
  • 說(shuō)明書：
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 其他：
  • 中文名稱：
    人TRMT61B重組蛋白
  • 貨號(hào)：
    CSB-EP863133HU-B
  • 說(shuō)明書：
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    人TRMT61B重組蛋白
  • 貨號(hào)：
    CSB-BP863133HU
  • 說(shuō)明書：
  • 規(guī)格：
  • 來(lái)源：
    Baculovirus
  • 其他：
  • 中文名稱：
    人TRMT61B重組蛋白
  • 貨號(hào)：
    CSB-MP863133HU
  • 說(shuō)明書：
  • 規(guī)格：
  • 來(lái)源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    TRMT61B
  • Uniprot No.：
  • 別名：
    TRMT61B; tRNA; adenine(58)-N(1))-methyltransferase; mitochondrial; EC 2.1.1.220; mRNA methyladenosine-N(1)-methyltransferase; EC 2.1.1.-
  • 種屬：
    Homo sapiens (Human)
  • 蛋白長(zhǎng)度：
    Full Length of Mature Protein
  • 表達(dá)區(qū)域：
    -
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Methyltransferase that catalyzes the formation of N(1)-methyladenine at position 58 (m1A58) in various tRNAs in mitochondrion, including tRNA(Leu) (deciphering codons UUA or UUG), tRNA(Lys) and tRNA(Ser) (deciphering codons UCA, UCU, UCG or UCC). Catalyzes the formation of 1-methyladenosine at position 947 of mitochondrial 16S ribosomal RNA and this modification is most likely important for mitoribosomal structure and function. In addition to tRNA N(1)-methyltransferase activity, also acts as a mRNA N(1)-methyltransferase by mediating methylation of adenosine residues at the N(1) position of MT-ND5 mRNA, leading to interfere with mitochondrial translation.
  • 基因功能參考文獻(xiàn)：
    1. 16S rRNA RNA-DNA differences result from a 1-methyladenosine (m1A) modification introduced by TRMT61B. PMID: 27631568
    2. Unlike the cytoplasmic tRNA m(1)A58 methyltransferase that consists of an alpha2beta2 heterotetramer formed by Trmt61A and Trmt6, Trmt61B formed a homo-oligomer (presumably a homotetramer) that resembled the bacterial homotetrameric m(1)A58 methyltransferase. PMID: 23097428
  • 亞細(xì)胞定位：
    Mitochondrion.
  • 蛋白家族：
    Class I-like SAM-binding methyltransferase superfamily, TRM61 family
  • 數(shù)據(jù)庫(kù)鏈接：

    HGNC: 26070

    KEGG: hsa:55006

    STRING: 9606.ENSP00000302801

    UniGene: Hs.468026



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