在线日韩日本国产亚洲丨少妇伦子伦情品无吗丨欧美性猛交xxxx免费看蜜桃丨精品人妻系列无码一区二区三区丨亚洲精品无码不卡在线播放

Your Good Partner in Biology Research

  1. 首頁
  2. 蛋白
  3. 重組蛋白
  4. Recombinant Human Polyribonucleotide nucleotidyltransferase 1, mitochondrial (PNPT1)

Recombinant Human Polyribonucleotide nucleotidyltransferase 1, mitochondrial (PNPT1)

  • 中文名稱：
    人PNPT1重組蛋白
  • 貨號：
    CSB-YP840567HU
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    人PNPT1重組蛋白
  • 貨號：
    CSB-EP840567HU
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    人PNPT1重組蛋白
  • 貨號：
    CSB-EP840567HU-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    人PNPT1重組蛋白
  • 貨號：
    CSB-BP840567HU
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    人PNPT1重組蛋白
  • 貨號：
    CSB-MP840567HU
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    PNPT1
  • Uniprot No.：
  • 別名：
    PNPT1; PNPASE; Polyribonucleotide nucleotidyltransferase 1; mitochondrial; EC 2.7.7.8; 3'-5' RNA exonuclease OLD35; PNPase old-35; Polynucleotide phosphorylase 1; PNPase 1; Polynucleotide phosphorylase-like protein
  • 種屬：
    Homo sapiens (Human)
  • 蛋白長度：
    Full Length of Mature Protein
  • 表達區域：
    46-783
  • 氨基酸序列
    AVAVD LGNRKLEISS GKLARFADGS AVVQSGDTAV MVTAVSKTKP SPSQFMPLVV DYRQKAAAAG RIPTNYLRRE IGTSDKEILT SRIIDRSIRP LFPAGYFYDT QVLCNLLAVD GVNEPDVLAI NGASVALSLS DIPWNGPVGA VRIGIIDGEY VVNPTRKEMS SSTLNLVVAG APKSQIVMLE ASAENILQQD FCHAIKVGVK YTQQIIQGIQ QLVKETGVTK RTPQKLFTPS PEIVKYTHKL AMERLYAVFT DYEHDKVSRD EAVNKIRLDT EEQLKEKFPE ADPYEIIESF NVVAKEVFRS IVLNEYKRCD GRDLTSLRNV SCEVDMFKTL HGSALFQRGQ TQVLCTVTFD SLESGIKSDQ VITAINGIKD KNFMLHYEFP PYATNEIGKV TGLNRRELGH GALAEKALYP VIPRDFPFTI RVTSEVLESN GSSSMASACG GSLALMDSGV PISSAVAGVA IGLVTKTDPE KGEIEDYRLL TDILGIEDYN GDMDFKIAGT NKGITALQAD IKLPGIPIKI VMEAIQQASV AKKEILQIMN KTISKPRASR KENGPVVETV QVPLSKRAKF VGPGGYNLKK LQAETGVTIS QVDEETFSVF APTPSAMHEA RDFITEICKD DQEQQLEFGA VYTATITEIR DTGVMVKLYP NMTAVLLHNT QLDQRKIKHP TALGLEVGQE IQVKYFGRDP ADGRMRLSRK VLQSPATTVV RTLNDRSSIV MGEPISQSSS NSQ
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    RNA-binding protein implicated in numerous RNA metabolic processes. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'-to-5' direction. Mitochondrial intermembrane factor with RNA-processing exoribonulease activity. Component of the mitochondrial degradosome (mtEXO) complex, that degrades 3' overhang double-stranded RNA with a 3'-to-5' directionality in an ATP-dependent manner. Involved in the degradation of non-coding mitochondrial transcripts (MT-ncRNA) and tRNA-like molecules. Required for correct processing and polyadenylation of mitochondrial mRNAs. Plays a role as a cytoplasmic RNA import factor that mediates the translocation of small RNA components, like the 5S RNA, the RNA subunit of ribonuclease P and the mitochondrial RNA-processing (MRP) RNA, into the mitochondrial matrix. Plays a role in mitochondrial morphogenesis and respiration; regulates the expression of the electron transport chain (ETC) components at the mRNA and protein levels. In the cytoplasm, shows a 3'-to-5' exoribonuclease mediating mRNA degradation activity; degrades c-myc mRNA upon treatment with IFNB1/IFN-beta, resulting in a growth arrest in melanoma cells. Regulates the stability of specific mature miRNAs in melanoma cells; specifically and selectively degrades miR-221, preferentially. Plays also a role in RNA cell surveillance by cleaning up oxidized RNAs. Binds to the RNA subunit of ribonuclease P, MRP RNA and miR-221 microRNA.
  • 基因功能參考文獻：
    1. The PNPT1 p.Arg136His and p.Pro140Leu variants in our subject showed 1) the mutated amino acids are highly conserved and our structural analysis supported them to be functionally deleterious; 2) The carrier frequencies of the variants are very low in populations (1:60.000), with no homozygous carriers found; 3) The RC complex amounts in the subject's myoblasts were functionally rescued by expression of wild-type PNPT1. PMID: 28645153
    2. Inhibition of homologous PNPase by citrate may represent an evolutionarily conserved communicative link between RNA degradation and central metabolism. PMID: 28334892
    3. In vitro rescue experiments, using exogenous expression of wild-type PNPT1 in patient fibroblasts, ameliorated the deficiencies in the OXPHOS complex protein expression, supporting the likely pathogenicity of these variants and the importance of Whole-exome sequencingin efficiently identifying rare genetic disease genes PMID: 27759031
    4. this study provides further evidence that hPNPase(old-35) is associated with global changes in cell cycle-associated genes and identifies potential gene targets for future investigation PMID: 24729470
    5. fresh insight into cellular pathways regulated by PNPT1 PMID: 24143183
    6. Interaction between PNPase and hSuv3 is essential for efficient mitochondrial RNA degradation. PMID: 23221631
    7. A mutation in PNPT1, encoding mitochondrial-RNA-import protein PNPase, causes hereditary hearing loss. PMID: 23084290
    8. Mutation in PNPT1, which encodes a polyribonucleotide nucleotidyltransferase, impairs RNA import into mitochondria and causes respiratory-chain deficiency. PMID: 23084291
    9. a novel role of nEGFR in radioresistance, and that is, upon ionizing radiation, nEGFR inactivates the ribonuclease activity of PNPase toward c-MYC mRNA through DNAPK-mediated Ser-776 phosphorylation PMID: 22815474
    10. The study provides structural and functional insights into hPNPase, which uses a KH pore to trap a long RNA 3' tail that is further delivered into an RNase PH channel for the degradation process. PMID: 22210891
    11. targeted overexpression of hPNPase(old-35) represents a novel strategy to selectively downregulate RNA expression and consequently intervene in a variety of pathophysiological conditions PMID: 21151174
    12. The data support an unanticipated role for PNPASE in mediating the translocation of RNAs into mitochondria. PMID: 20691904
    13. Inhibition of PNPase by shRNA or stable overexpression of miR-221 protected melanoma cells from IFN-beta-mediated growth inhibition, accentuating the importance of PNPase induction and miR-221 down-regulation in mediating IFN-beta action. PMID: 20547861
    14. Protein-protein interactions between human exosome components support the assembly of RNase PH-type subunits into a six-membered PNPase-like ring PMID: 12419256
    15. an important role for hPNPase(old-35)in growth control associated with terminal differentiation and cellular senescence. PMID: 12473748
    16. the molecular mechanism of the growth-arresting property of hPNPaseold-35 PMID: 12721301
    17. demonstrate that the hPNPase is localized in mitochondria; finding suggests the involvement of mitochondrial RNA metabolism in cellular senescence PMID: 12798676
    18. analysis of domains of human polynucleotide phosphorylase (hPNPaseOLD-35) mediating cellular senescence PMID: 16055741
    19. Targeted overexpression of hPNPase(old-35) might be a novel therapeutic strategy for c-myc-overexpressing and IFN-resistant tumors, such as melanomas. PMID: 16410805
    20. human PNPase mRNA upregulation by beta-interferon has no effect on protein level in melanoma cell lines PMID: 16505900
    21. PNPase localization to the mitochondrial intermembrane space suggests a unique role distinct from its highly conserved function in RNA processing in chloroplasts and bacteria. PMID: 16966379
    22. Combined, the data demonstrate an unexpected IMS localization and a key role for PNPase in maintaining mitochondrial homeostasis. PMID: 16966381
    23. A novel pathway by which an evolutionary conserved RNA-metabolizing enzyme, hPNPase(old-35), regulates cell growth and viability. PMID: 17804700
    24. recent advances in understanding the various roles of hPNPase both within and potentially outside of the mitochondria [review] PMID: 17983748
    25. In this work, the degradation, polymerization, and RNA-binding properties of the human PNPase were analyzed and compared to its bacterial and organellar counterparts. PMID: 18083836
    26. Involvement of human polynucleotide phosphorylase in mtRNA degradation was studied. PMID: 18083837
    27. The complex of hSUV3-hPNPase is an integral entity for efficient degradation of structured RNA and may be the long sought RNA-degrading complex in the mammalian mitochondria. PMID: 19509288
    28. maintained expression and even up-regulation of some (PNPT1, PMPCB, HMMR/RHAMM, BSG and ERCC1) tumor associated antigens in CD40-activated leukemic cells. PMID: 19580345

    顯示更多

    收起更多

  • 相關疾病：
    Combined oxidative phosphorylation deficiency 13 (COXPD13); Deafness, autosomal recessive, 70 (DFNB70)
  • 亞細胞定位：
    Cytoplasm. Mitochondrion matrix. Mitochondrion intermembrane space; Peripheral membrane protein.
  • 蛋白家族：
    Polyribonucleotide nucleotidyltransferase family
  • 數據庫鏈接：

    HGNC: 23166

    OMIM: 610316

    KEGG: hsa:87178

    STRING: 9606.ENSP00000393953

    UniGene: Hs.388733



主站蜘蛛池模板： 中文综合在线观| 乱子伦视频在线看| 久久香蕉国产线看观看精品yw| 内射口爆少妇麻豆| 亚洲精品日韩一区二区电影| 9九色桋品熟女内射| 色综合av综合无码综合网站| 日韩一区二区三区北条麻妃| 国产婷婷亚洲999精品小说| 好爽...又高潮了毛片| av无码久久久久不卡网站蜜桃| 国产精品无码无片在线观看| 在线播放亚洲第一字幕| 777精品久无码人妻蜜桃| 久久综合九色综合欧美婷婷| 日韩欧美tⅴ一中文字暮| 天堂av成年av影视| 久久久精品免费| av天堂永久资源网| 亚洲欧美日韩成人一区二区三区| 国内精品久久久久影院中文字幕| 精品国产肉丝袜久久| 亚洲日韩中文字幕在线播放| 久久人妻av一区二区软件| 国产日韩综合一区二区性色av| 欧美日韩不卡高清在线看| 亚洲欧美成αⅴ人在线观看| 国产精品亚洲а∨无码播放麻豆 | 波多野美乳人妻hd电影欧美| 久激情内射婷内射蜜桃| 亚洲综合小说另类图片五月天| 亚洲性色成人av| 精选国产av精选一区二区三区| 性欧美欧美巨大69| 国产精品自在线拍国产| 亚洲欧美日本中文字不卡| 久久ww精品w免费人成| 伦伦影院午夜理论片| 人妻无码一区二区三区免费| 国产精品国产对白熟妇| 天天躁日日躁aaaaxxxx|