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Recombinant Human PR domain zinc finger protein 13 (PRDM13)

  • 中文名稱:
    人PRDM13重組蛋白
  • 貨號:
    CSB-YP887981HU
  • 規格:
  • 來源:
    Yeast
  • 其他:
  • 中文名稱:
    人PRDM13重組蛋白
  • 貨號:
    CSB-EP887981HU
  • 規格:
  • 來源:
    E.coli
  • 其他:
  • 中文名稱:
    人PRDM13重組蛋白
  • 貨號:
    CSB-EP887981HU-B
  • 規格:
  • 來源:
    E.coli
  • 共軛:
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他:
  • 中文名稱:
    人PRDM13重組蛋白
  • 貨號:
    CSB-BP887981HU
  • 規格:
  • 來源:
    Baculovirus
  • 其他:
  • 中文名稱:
    人PRDM13重組蛋白
  • 貨號:
    CSB-MP887981HU
  • 規格:
  • 來源:
    Mammalian cell
  • 其他:

產品詳情

  • 純度:
    >85% (SDS-PAGE)
  • 基因名:
    PRDM13
  • Uniprot No.:
  • 別名:
    PRDM13; PFM10; PR domain zinc finger protein 13; EC 2.1.1.-; PR domain-containing protein 13
  • 種屬:
    Homo sapiens (Human)
  • 蛋白長度:
    full length protein
  • 表達區域:
    1-707
  • 氨基酸序列
    MHGAARAPAT SVSADCCIPA GLRLGPVPGT FKLGKYLSDR REPGPKKKVR MVRGELVDES GGSPLEWIGL IRAARNSQEQ TLEAIADLPG GQIFYRALRD VQPGEELTVW YSNSLAQWFD IPTTATPTHD EKGEERYICW YCWRTFRYPN SLKAHLRFHC VFSGGGGGAF LHHEHAARQG AVPAADGLGL SPKPPAPDFA APSQAGTLRP HPLGPPPVQA CGAREGIKRE ASSAPSATSP TPGKWGQPKK GKEQLDRALD MSGAARGQGH FLGIVGGSSA GVGSLAFYPG VRSAFKPAGL ARAAAAAHGD PYREESSSKQ GAGLALGRLL GGGRACGRPG SGENSAAGGA GHHHHHHAHH HHHPKCLLAG DPPPPPPPGL PCSGALRGFP LLSVPPEEAS AFKHVERAPP AAAALPGARY AQLPPAPGLP LERCALPPLD PGGLKAYPGG ECSHLPAVMP AFTVYNGELL YGSPATTAYY PLKLHFGGLL KYPESISYFS GPAAAALSPA ELGSLASIDR EIAMHNQQLS EMAAGKGRGR LDSGTLPPAV AAAGGTGGGG SGGSGAGKPK TGHLCLYCGK LYSRKYGLKI HMRTHTGYKP LKCKVCLRPF GDPSNLNKHI RLHAEGNTPY RCEFCGKVLV RRRDLERHVK SRHPGQSLLA KAGDGPGAEP GYPPEPGDPK SDDSDVDVCF TDDQSDPEVG GGGERDL
  • 蛋白標簽:
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式:
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet :
    Please contact us to get it.

產品評價

問答及客戶評論

 常見問題解答
Q:

Can this protein provide in Tris buffer 50% glycerol, could it be provided as lyophilized either as 50 ug or 100 ug at 0.5 ug/ul concentration (or higher)? That is, could you deliver 50 ug in 100 ul or 100 ug in 200 ul (or less)?

A:
Thanks for your inquiry.Regarding CSB-BP887981HU, 0.05mg and 0.1mg, we can provide it in liquid form with buffer: Tris buffer 50% glycerol,
and we can meet your requirement for the concentration of 0.5mg/ml namely 0.5 ug/ul or higher. Pls remark your requirements when placing the order.
We can also provide it in lyophilized form if you need, the lead time will be extended for 3 working days.
If you choose the lyophilized form, then the final concentration will be adjusted by yourself after you dissolve the protein.
Reconstitution of Lyophilized Proteins:
a.The reconstitution method is the same for all proteins. Please use deionized sterile water to reconstitute proteins.
(Dissolve completely. The concentration could depend on customers' experiment demand.)
b.It is better that concentration does not be lower than 0.1mg/ml. Proteins will be unstable if they're too diluted. Generally, we recommend 0.1-1.0mg/ml concentration.
If customer has special requirements for concentration, then they could dilute the protein according to their experiment demand.
c.We haven’t studied whether the optimal concentration range will vary among different hosts such as E.coli, yeast, mammalian or baculovirus, so we can’t provide useful advice.
d.If customer will use immediately after dissolving one protein, then they don't need to add glycerol. If they won't use the protein in short term, we suggest to add some glycerol for storage. We suggest customer to reconstitute suitable amount of protein and store the remaining protein in lyophilized powder. You could remark "The lyophilized powder should be packed into multiple vials" when placing order. The amount of lyophilized powder in each vial will depend on customer's experiment demand.
e.In principle, the final concentration of glycerol from 5% to 50% all will be OK. Our default final concentration of glycerol is 50%. Customers could use it as reference.

靶點詳情

  • 功能:
    May be involved in transcriptional regulation.
  • 基因功能參考文獻:
    1. The over-expression of PRDM13 upregulated deleted in liver cancer 1 (DLC1) to inhibit the proliferation and invasion of U87 cells. PMID: 29767251
    2. The duplication found in the RFS355 family is distinct from the previously reported duplication and provides additional support that dysregulation of PRDM13, not CCNC, is the cause of NCMD mapped to the MCDR1 locus. PMID: 27777503
    3. Findings indicate that Prdm13/Nkx2-1-mediated signaling in the DMC declines with advanced age, leading to decreased sleep quality and increased adiposity. PMID: 25546159
  • 亞細胞定位:
    Nucleus.
  • 蛋白家族:
    Class V-like SAM-binding methyltransferase superfamily
  • 數據庫鏈接:

    HGNC: 13998

    KEGG: hsa:59336

    STRING: 9606.ENSP00000358217

    UniGene: Hs.287386



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