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Recombinant Human Omega-amidase NIT2 (NIT2)

  • 中文名稱:
    人NIT2重組蛋白
  • 貨號:
    CSB-YP882075HU
  • 規格:
  • 來源:
    Yeast
  • 其他:
  • 中文名稱:
    人NIT2重組蛋白
  • 貨號:
    CSB-EP882075HU
  • 規格:
  • 來源:
    E.coli
  • 其他:
  • 中文名稱:
    人NIT2重組蛋白
  • 貨號:
    CSB-EP882075HU-B
  • 規格:
  • 來源:
    E.coli
  • 共軛:
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他:
  • 中文名稱:
    人NIT2重組蛋白
  • 貨號:
    CSB-BP882075HU
  • 規格:
  • 來源:
    Baculovirus
  • 其他:
  • 中文名稱:
    人NIT2重組蛋白
  • 貨號:
    CSB-MP882075HU
  • 規格:
  • 來源:
    Mammalian cell
  • 其他:

產品詳情

  • 純度:
    >85% (SDS-PAGE)
  • 基因名:
  • Uniprot No.:
  • 別名:
    CUA002; MGC111199; NIT 2; Nit protein 2; NIT2; NIT2_HUMAN; Nitrilase family member 2; Nitrilase homolog 2; Omega amidase NIT2; Omega-amidase NIT2
  • 種屬:
    Homo sapiens (Human)
  • 蛋白長度:
    full length protein
  • 表達區域:
    1-276
  • 氨基酸序列
    MTSFRLALIQ LQISSIKSDN VTRACSFIRE AATQGAKIVS LPECFNSPYG AKYFPEYAEK IPGESTQKLS EVAKECSIYL IGGSIPEEDA GKLYNTCAVF GPDGTLLAKY RKIHLFDIDV PGKITFQESK TLSPGDSFST FDTPYCRVGL GICYDMRFAE LAQIYAQRGC QLLVYPGAFN LTTGPAHWEL LQRSRAVDNQ VYVATASPAR DDKASYVAWG HSTVVNPWGE VLAKAGTEEA IVYSDIDLKK LAEIRQQIPV FRQKRSDLYA VEMKKP
  • 蛋白標簽:
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式:
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet :
    Please contact us to get it.

產品評價

靶點詳情

  • 功能:
    Has omega-amidase activity. The role of omega-amidase is to remove potentially toxic intermediates by converting 2-oxoglutaramate and 2-oxosuccinamate to biologically useful 2-oxoglutarate and oxaloacetate, respectively.
  • 基因功能參考文獻:
    1. Downregulation of NIT2 inhibits colon cancer cell proliferation and induces cell cycle arrest through the caspase-3 and PARP pathways PMID: 25738796
    2. Human NIT1/2 are not involved in the metabolism of vildagliptin. PMID: 25008847
    3. The activity and substrate specificity of human Nit2 was determined with alpha-ketoglutaramate and succinamate as substrates. PMID: 22674578
    4. Genotype analysis in 4 types of tumor tissue showed allelic imbalance surrounding NIT2 locus; results show that NIT2 plays an important role in cell growth inhibition & links to malignancies, suggesting Nit2 may be a potential tumor suppressor candidate PMID: 17488281
  • 亞細胞定位:
    Cytoplasm.
  • 蛋白家族:
    Carbon-nitrogen hydrolase superfamily, NIT1/NIT2 family
  • 組織特異性:
    Detected in fetal brain (at protein level). Ubiquitous. Detected in heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, prostate, spleen, thymus, prostate, testis, ovary, small intestine and colon.
  • 數據庫鏈接:

    HGNC: 29878

    KEGG: hsa:56954

    STRING: 9606.ENSP00000377696

    UniGene: Hs.439152



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