Recombinant Human Mitogen-activated protein kinase kinase kinase 14 (MAP3K14), partial

^{In Stock}

中文名稱：

人MAP3K14重組蛋白
貨號：

CSB-EP013422HU
規格：

￥1836
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

Greater than 85% as determined by SDS-PAGE.
基因名：

MAP3K14
Uniprot No.：

Q99558
別名：

Serine/threonine-protein kinase NIK NF-kappa-beta-inducing kinase
種屬：

Homo sapiens (Human)
蛋白長度：

Partial
來源：

E.coli
分子量：

35.4 kDa
表達區域：

400-655aa
氨基酸序列

ATHQLRLGRGSFGEVHRMEDKQTGFQCAVKKVRLEVFRAEELMACAGLTSPRIVPLYGAVREGPWVNIFMELLEGGSLGQLVKEQGCLPEDRALYYLGQALEGLEYLHSRRILHGDVKADNVLLSSDGSHAALCDFGHAVCLQPDGLGKSLLTGDYIPGTETHMAPEVVLGRSCDAKVDVWSSCCMMLHMLNGCHPWTQFFRGPLCLKIASEPPPVREIPPSCAPLTAQAIQEGLRKEPIHRVSAAELGGKVNRAL
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 10xHis-tagged and C-terminal Myc-tagged
產品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

Lymphotoxin beta-activated kinase which seems to be exclusively involved in the activation of NF-kappa-B and its transcriptional activity. Promotes proteolytic processing of NFKB2/P100, which leads to activation of NF-kappa-B via the non-canonical pathway. Could act in a receptor-selective manner.
基因功能參考文獻：
1. A TRAF3-NIK axis differentially regulates viral DNA vs RNA pathways in innate immune signaling. PMID: 30018345
2. Data show that breast cancer stem cells (BCSCs) expressed higher levels of NF-kappaB-inducing kinase (NIK). PMID: 27876836
3. These findings highlight the importance of NIK in tumor pathogenesis and invite new therapeutic strategies that attenuate mitochondrial dysfunction through inhibition of NIK and Drp1. PMID: 27889261
4. results suggest that changes in the relative concentrations of RelB, NIK:IKK1, and p100 during noncanonical signaling modulate this transitional complex and are critical for maintaining the fine balance between the processing and protection of p100. PMID: 27678221
5. report a detailed state-of-the-art mass spectrometry-based protein-protein interaction network including the noncanonical NF-kappaB signaling nodes TRAF2, TRAF3, IKKalpha, NIK, and NF-kappaB2/p100; also provide a differential interactome of NIK mutants that cause immunodeficiency PMID: 27416764
6. OLFM1 is a negative regulator of non-canonical NF-kappaB signalling by interacting with and inhibiting NIK. Thus, OLFM1 may serve as a valuable biomarker and therapeutic target for colorectal cancer (CRC) patients. PMID: 27555280
7. The expression of OTUD7B and NIK were negatively correlated in non-small cell lung cancer tumor samples. the higher expression of NIK was related to more lymph node metastasis and later TNM stage. high OTUD7B/low NIK index can predict an good prognosis. PMID: 27499151
8. NIK expression was significantly increased in the tumor tissue of patients with breast carcinoma, which may be an important factor that affects the prognosis of these patients. PMID: 26823811
9. This study identified two novel independent loci (MAP3K14 and CARD9) strongly associated with joint damage in Mexican Americans and European Americans and a few shared loci showing suggestive evidence for association. PMID: 26498133
10. The forced expression of NDRG2 in ATL cells down-regulates not only the canonical pathway by inhibiting AKT signaling but also the non-canonical pathway by inducing NF-kappaB-inducing kinase (NIK) dephosphorylation via the recruitment of PP2A PMID: 26269411
11. NIK(+) endothelial cells may play an important role in the persistence of synovitis PMID: 26178280
12. Data suggest microRNA302c, but not microRNA520e, promotes replication of influenza A virus H3N2 although the two microRNAs target same site of NFkappaB-inducing kinase (MAP3K14) 3prime untranslated region; studies were conducted in lung epithelial cells. PMID: 26602079
13. by demonstrating a critical role of NIK in mediating NF-kappaB activation and BAG3 induction upon ST80/Bortezomib cotreatment, our study provides novel insights into mechanisms of resistance to proteotoxic stress in RMS PMID: 25766331
14. Loss-of-function mutations in NIK can cause B-cell lymphopenia, hypogammaglobulinemia, impaired ICOSL expression, and disordered T helper cell and NK cell function. PMID: 25406581
15. Membrane attack complexes activate noncanonical NF-kappaB by forming a novel Akt(+)NIK(+) signalosome on Rab5(+) endosomes. PMID: 26195760
16. Authors show that activation of NF-kappaB by Kaposi's sarcoma-associated herpesvirus K15 protein involves the recruitment of NF-kappaB-inducing kinase (NIK) and IKK alpha/beta to result in the phosphorylation of p65/RelA on Ser536. PMID: 25187543
17. NIK plays a key role in constitutive NF-kappaB activation and the progression of ovarian cancer cells PMID: 24533079
18. Identification and function of the NIK IAP binding motif, which promotes c-IAP1-dependent ubiquitylation of NIK. PMID: 25246529
19. These studies provide evidence for a role of NF-kappaB-inducing kinase (NIK) and subsequent non-canonical NF-kappaB signalling in pathological angiogenesis PMID: 25043127
20. Results show that nuclear factor-kappa B inducing kinase has a role in graft-versus-host disease by maintaining the viability of activated alloreactive T lymphocytes. PMID: 20823135
21. NIK is a new therapeutic target for Mantle cell lymphoma treatment, particularly for lymphomas that are refractory to B cell receptor pathway inhibitors. PMID: 24362935
22. A pivotal role for NIK in nuclear factor kappa B activation and regulation of gene expression in T-cell lymphoma. PMID: 23536439
23. TRAF2/NIK/NF-kappaB2 pathway regulates pancreatic ductal adenocarcinoma cell tumorigenicity PMID: 23301098
24. APPL1 regulates basal NF-kappaB activity by modulating the stability of NIK, which affects the activation of p65. PMID: 22685329
25. we found that NIK and the noncanonical pathway are very prevalent in Hodgkin lymphoma PMID: 22968463
26. study shows API2-MALT1 fusion oncoprotein induces proteolytic cleavage of NF-kappaB-inducing kinase (NIK); resulting deregulated NIK activity is associated with constitutive noncanonical NF-kappaB signaling, enhanced B cell adhesion and apoptosis resistance PMID: 21273489
27. a molecular basis for the recent observation of gain-of-function activity for an N-terminal deletion mutant (DeltaN324) of NIK, leading to constitutive non-canonical NF-kappaB signaling with enhanced B-cell adhesion and apoptosis resistance. PMID: 22718757
28. NIK is one of the direct target genes of miR-520e. PMID: 22105365
29. NIK mediates both beta-catenin and NF-kappaB regulated transcription to modulate melanoma survival and growth PMID: 21963849
30. Genetic lesions of the TRAF3 and MAP3K14 genes in classical Hodgkin lymphoma. PMID: 22469134
31. NIK activity in nonhematopoietic cells controls Th2 cell development and prevents eosinophil-driven inflammatory disease, most likely using a signaling pathway that operates independent of the known NIK substrate IKKalpha. PMID: 22474019
32. Cigarette smoke and TNFalpha increase NIK levels causing phosphorylation of IKKalpha, which leads to histone acetylation. PMID: 21887257
33. A role for NF-kappaB noncanonical pathway activation in modulating diabetes-induced inflammation in renal tubular epithelium. PMID: 21869881
34. Adiponectin treatment inhibited NIK-induced NF-kappaB activation and restored insulin sensitivity by restoring phosphatidylinositol 3 kinase activation and subsequent serine-threonine kinase phosphorylation. PMID: 21846802
35. findings suggest that the CC genotype of NIK rs7222094 is associated with increased mortality and organ dysfunction in septic shock patients, perhaps due to altered regulation of NF-kappaB pathway genes, including CXCL10 PMID: 21257964
36. plays a key role in constitutive NF-kappaB activation in non-small cell lung cancer cells PMID: 20338663
37. TNF-alpha and carrageenan in combination act to increase NIK phosphorylation, thereby increasing activation of the non-canonical pathway of NF-kappaB activation PMID: 20937806
38. Data suggest that NIK and genes induced by the NIK-mediated constitutive NF-kappaB activation could be therapeutic targets of basal-like breast cancer. PMID: 20735436
39. An atypical E3 ligase zinc finger protein 91 stabilizes and activates NF-kappaB-inducing kinase via Lys63-linked ubiquitination. PMID: 20682767
40. Data show that NIK with mutations in the IKKalpha-targeted serine residues was more stable than wild-type NIK and resulted in increased noncanonical NF-kappaB signaling. PMID: 20501937
41. These results suggest that augmentation of NF-kappaB-inducing kinase, IkappaB kinase-alpha, and pH3 in response to oxidative stress is involved in cell death after cerebral ischemia (or stroke). PMID: 20125184
42. the LTbetaR modifies the ubiquitin:NIK E3 ligase, and also acts as an allosteric regulator of the ubiquitin:TRAF E3 ligase PMID: 20348096
43. Data provide analysis of the IKK/NIK inter-relationship and its impact on NF-kappaB control by mathematical modelling. PMID: 19909783
44. results demonstrate that IKK2 is essential for dendritic cell activation induced by CD40L or contact with allogeneic T cells, but not by LPS, whereas NIK is not required for any of these signals PMID: 12393548
45. induction of noncanonical NF-kappaB signaling may involve the rescue of NIK from TRAF3-mediated negative regulation PMID: 15084608
46. subcellular distribution of NIK to different compartments might be a means of regulating the function of this kinase PMID: 15252129
47. Results for the first time place NF-kappa B-inducing kinase (NIK), previously shown to function in TNF signaling, within the interferon signal transduction pathway PMID: 16009713
48. This study demonstrates that NF-kappaB-inducing kinase (NIK) is a critical target of the endogenous ROS in NF-kappaB pathways. PMID: 17729113
49. NF-kappaB-inducing kinase phosphorylates and blocks the degradation of Down syndrome candidate region 1 PMID: 18056702
50. NIK functions downstream of Cot to mediate p65 phosphorylation. PMID: 18439422
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亞細胞定位：

Cytoplasm.
蛋白家族：

Protein kinase superfamily, STE Ser/Thr protein kinase family, MAP kinase kinase kinase subfamily
組織特異性：

Weakly expressed in testis, small intestine, spleen, thymus, peripheral blood leukocytes, prostate, ovary and colon.
數據庫鏈接：

HGNC: 6853

OMIM: 604655

KEGG: hsa:9020

UniGene: Hs.404183