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  4. Recombinant Human Meiotic recombination protein SPO11 (SPO11)

Recombinant Human Meiotic recombination protein SPO11 (SPO11)

  • 中文名稱：
    人SPO11重組蛋白
  • 貨號：
    CSB-YP897506HU
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    人SPO11重組蛋白
  • 貨號：
    CSB-EP897506HU-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    人SPO11重組蛋白
  • 貨號：
    CSB-MP897506HU
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    SPO11
  • Uniprot No.：
  • 別名：
    Cancer/testis antigen 35; CT35; Meiotic recombination protein SPO 11; Meiotic recombination protein SPO11; MGC39953; SPATA43; Spermatogenesis associated 43; SPO 11; SPO11; SPO11 meiotic protein covalently bound to DSB; SPO11 meiotic protein covalently bound to DSB homolog; SPO11 meiotic protein covalently bound to DSB like; SPO11_HUMAN; TOPVIA
  • 種屬：
    Homo sapiens (Human)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-396
  • 氨基酸序列
    MAFAPMGPEA SFFDVLDRHR ESLLAALRRG GREPPTGGSR LASSSEVLAS IENIIQDIIT SLARNEAPAF TIDNRSSWEN IKFEDSVGLQ MVSHCTTRKI KSDSPKSAQK FSLILKILSM IYKLVQSNTY ATKRDIYYTD SQLFGNQTVV DNIINDISCM LKVSRRSLHI LSTSKGLIAG NLRYIEEDGT KVNCTCGATA VAVPSNIQGI RNLVTDAKFV LIVEKDATFQ RLLDDNFCNK LSPCIMITGK GVPDLNTRLL VKKLWDTFHV PVFTLVDADP HGIEIMCIYK YGSMSMSFEA HHLTVPAIRW LGLLPSDLKR LNVPKDSLIP LTKRDQMKLD SILRRPYVTC QPFWRKEMEI MADSKMKAEI QALTFLSSDY LSRVYLPNKL KFGGWI
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Component of a topoisomerase 6 complex specifically required for meiotic recombination. Together with TOP6BL, mediates DNA cleavage that forms the double-strand breaks (DSB) that initiate meiotic recombination. The complex promotes relaxation of negative and positive supercoiled DNA and DNA decatenation through cleavage and ligation cycles. Essential for the phosphorylation of SMC3, HORMAD1 and HORMAD2.
  • 基因功能參考文獻：
    1. SPO11 gene C631T polymorphism may contribute as a genetic factor susceptible to cause male infertility in Chinese. PMID: 28050928
    2. Data suggest that the combined genotypes of glutathionine S-transferase GSTM1 (-/-), GSTT1 (+/+), GSTP1 (AA) and meiotic recombination protein SPO11 (CT) may be associated with idiopathic male infertility in ethnic Han Chinese. PMID: 26663067
    3. Polymorphisms within the SPO11 gene are linked to the susceptibility of azoospermia and oligozoospermia male infertility. PMID: 25005169
    4. SPO11 might has an effect on premorbid functioning, which increase susceptibility for idiopathic male reproduction PMID: 21556891
    5. Mutations in the human SPO11 gene are not common causes of infertility in man PMID: 16169419
    6. Data show that there is no association between SPO11 gene mutation and premature ovarian failure. PMID: 18166824

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  • 亞細胞定位：
    Nucleus.
  • 蛋白家族：
    TOP6A family
  • 組織特異性：
    Highly expressed in testis.
  • 數據庫鏈接：

    HGNC: 11250

    OMIM: 605114

    KEGG: hsa:23626

    STRING: 9606.ENSP00000360310

    UniGene: Hs.159737



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