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Recombinant Human Mediator of RNA polymerase II transcription subunit 30 (MED30)

  • 中文名稱:
    人MED30重組蛋白
  • 貨號:
    CSB-YP822225HU
  • 規格:
  • 來源:
    Yeast
  • 其他:
  • 中文名稱:
    人MED30重組蛋白
  • 貨號:
    CSB-EP822225HU
  • 規格:
  • 來源:
    E.coli
  • 其他:
  • 中文名稱:
    人MED30重組蛋白
  • 貨號:
    CSB-EP822225HU-B
  • 規格:
  • 來源:
    E.coli
  • 共軛:
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他:
  • 中文名稱:
    人MED30重組蛋白
  • 貨號:
    CSB-BP822225HU
  • 規格:
  • 來源:
    Baculovirus
  • 其他:
  • 中文名稱:
    人MED30重組蛋白
  • 貨號:
    CSB-MP822225HU
  • 規格:
  • 來源:
    Mammalian cell
  • 其他:

產品詳情

  • 純度:
    >85% (SDS-PAGE)
  • 基因名:
    MED30
  • Uniprot No.:
  • 別名:
    MED30; MED30_HUMAN; Mediator complex subunit 30; Mediator of RNA polymerase II transcription subunit 30; THRAP6; Thyroid hormone receptor-associated protein 6; Thyroid hormone receptor-associated protein complex 25 kDa component; TRAP/Mediator complex component TRAP25; Trap25
  • 種屬:
    Homo sapiens (Human)
  • 蛋白長度:
    Full Length of Mature Protein
  • 表達區域:
    2-178
  • 氨基酸序列
    STPPLAASG MAPGPFAGPQ AQQAAREVNT ASLCRIGQET VQDIVYRTME IFQLLRNMQL PNGVTYHTGT YQDRLTKLQD NLRQLSVLFR KLRLVYDKCN ENCGGMDPIP VEQLIPYVEE DGSKNDDRAG PPRFASEERR EIAEVNKKLK QKNQQLKQIM DQLRNLIWDI NAMLAMRN
  • 蛋白標簽:
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式:
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet :
    Please contact us to get it.

產品評價

靶點詳情

  • 功能:
    Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
  • 基因功能參考文獻:
    1. MED30 seems to be involved in the progression of the renal cell carcinoma (RCC). PMID: 29661722
    2. Knockdown of MED30 led to reduction of the tumour parameters proliferation, migration, and invasion in the bladder cancer (BCa) cell lines. PMID: 29187445
    3. analysis of a novel transcript of MED30, termed MED30 short (MED30s) generated by alternative splicing PMID: 20493979
    4. Requirement of TRAP/mediator for both activator-independent and activator-dependent transcription in conjunction with TFIID-associated TAF(II)s. PMID: 11909976
  • 亞細胞定位:
    Nucleus.
  • 蛋白家族:
    Mediator complex subunit 30 family
  • 組織特異性:
    Expressed in brain, heart, kidney, liver, lung, pancreas, placenta and skeletal muscle.
  • 數據庫鏈接:

    HGNC: 23032

    OMIM: 610237

    KEGG: hsa:90390

    STRING: 9606.ENSP00000297347

    UniGene: Hs.492612



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