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  4. Recombinant Human Mediator of RNA polymerase II transcription subunit 14 (MED14), partial

Recombinant Human Mediator of RNA polymerase II transcription subunit 14 (MED14), partial

  • 中文名稱：
    人MED14重組蛋白
  • 貨號：
    CSB-YP013647HU
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    人MED14重組蛋白
  • 貨號：
    CSB-EP013647HU
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    人MED14重組蛋白
  • 貨號：
    CSB-EP013647HU-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    人MED14重組蛋白
  • 貨號：
    CSB-BP013647HU
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    人MED14重組蛋白
  • 貨號：
    CSB-MP013647HU
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    MED14
  • Uniprot No.：
  • 別名：
    Activator-recruited cofactor 150 kDa component; ARC150; Cofactor required for Sp1 transcriptional activation subunit 2; CRSP complex subunit 2; DRIP150; EXLM1; hRGR1; MED14; MED14_HUMAN; Mediator complex subunit 14; Mediator of RNA polymerase II transcription subunit 14; RGR1; RGR1 homolog; Thyroid hormone receptor-associated protein complex 170 kDa component; Transcriptional coactivator CRSP150; Trap170; Vitamin D3 receptor-interacting protein complex 150 kDa component
  • 種屬：
    Homo sapiens (Human)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
  • 基因功能參考文獻：
    1. Functional analysis implicates TNRC6A, NAT10, MED14, and WDR5 in RNA-mediated transcriptional activation. PMID: 28813667
    2. This results from a dramatically enhanced ability of MED14-containing complexes to associate with Pol II. PMID: 25383669
    3. VitD-mediated stimulation of GC anti-inflammatory affects human monocytes in a process involving GM-CSF and MED14 PMID: 23572530
    4. DRIP150 binds to ISGF3 and regulates transcription PMID: 12509459
    5. CRSP2 gene is expressed in the retina and its exact genomic location is on Xp11.4 between DXS1368 and DXS993 PMID: 12825353
    6. Coactivation of ERalpha by DRIP150 in ZR-75 cells is NR box-independent and requires a novel sequence with putative alpha-helical structure. PMID: 15625066
    7. MED14 and MED1 are used by glucocorticoid receptor in a gene-specific manner, providing a mechanism for promoter selectivity by glucocorticoid receptor PMID: 16239257
    8. Coactivator that enhances estrogen receptor alpha- and specificity protein (SP)-1-mediated transactivation in breast cancer cells. PMID: 17306756

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  • 亞細胞定位：
    Nucleus.
  • 蛋白家族：
    Mediator complex subunit 14 family
  • 組織特異性：
    Ubiquitous.
  • 數據庫鏈接：

    HGNC: 2370

    OMIM: 300182

    KEGG: hsa:9282

    STRING: 9606.ENSP00000323720

    UniGene: Hs.407604



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