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  4. Recombinant Human Immunoglobulin lambda-like polypeptide 1 (IGLL1)

Recombinant Human Immunoglobulin lambda-like polypeptide 1 (IGLL1)

  • 中文名稱：
    人IGLL1重組蛋白
  • 貨號：
    CSB-YP011471HU
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    人IGLL1重組蛋白
  • 貨號：
    CSB-EP011471HU
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    人IGLL1重組蛋白
  • 貨號：
    CSB-EP011471HU-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    人IGLL1重組蛋白
  • 貨號：
    CSB-BP011471HU
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    人IGLL1重組蛋白
  • 貨號：
    CSB-MP011471HU
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    IGLL1
  • Uniprot No.：
  • 別名：
    14.1; AGM2; CD179 antigen-like family member B; CD179b; CD179b antigen; Ig lambda 5 ; Ig lambda-5; IGL1 ; IGL5; IGLJ14.1 ; IGLL ; Igll1; IGLL1_HUMAN; IGO ; IGVPB ; Immunoglobulin lambda like polypeptide 1 precursor; Immunoglobulin lambda-like polypeptide 1; Immunoglobulin omega polypeptide; Immunoglobulin omega polypeptide chain; Immunoglobulin related protein 14.1 ; Immunoglobulin-related protein 14.1; Lambda5; Pre B lymphocyte specific protein 2 ; VPREB2
  • 種屬：
    Homo sapiens (Human)
  • 蛋白長度：
    Full Length of Mature Protein
  • 表達區域：
    38-213
  • 氨基酸序列
    LLR PTAASQSRAL GPGAPGGSSR SSLRSRWGRF LLQRGSWTGP RCWPRGFQSK HNSVTHVFGS GTQLTVLSQP KATPSVTLFP PSSEELQANK ATLVCLMNDF YPGILTVTWK ADGTPITQGV EMTTPSKQSN NKYAASSYLS LTPEQWRSRR SYSCQVMHEG STVEKTVAPA ECS
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

問答及客戶評論

■ 常見問題解答
Q:

Can you make this product with an epitope tag other than His tag?

A:
Thanks for your inquiry. Do you mean the protein only has the epitope tag without any other tag, or the customer can accept epitope tag as well as other tag.
The expected tag for each expression system is list as follows:
YP: N-terminal 6xHis-tagged; EP, BP, MP: N-terminal 10xHis-tagged and C-terminal Myc-tagged.
As you can see the default tag for each expression system, except the YP protein, the other three proteins all have the myc-tag at the C-terminal, it's an epitope tag,
Mainly for the convenience of customer and us to do myc-tag antibody WB detection, in order to further detect the correctness of the target protein.
If the customer wants to keep the C-terminal Myc-tagged and doesn't want N-terminal His-Tag, we can perform tag removal to remove the N-terminal His-tag.
The overall success rate of enzyme digestion data analysis is 75%-86%.
Not all protein tags can be removed as some proteins will be very unstable after tag removal.
If we succeed in removing the tag, we will charge for extra cost.
If we fail in removing the tag, we won’t charge for any extra cost, and remark this information in datasheet as follows
“Note: The laboratory determined that the Tag on your protein could not be removed with standard laboratory procedures. Your protein is being supplied with the Tag intact.”
Generally, the delivery time will be extended for 3 days.
If the customer needs some other epitope tags, such as Flag tag or HA tag, we can also provide. There isn't any extra charges, just need to remark your requirement when placing the order.
Regarding the Flag-tag, there are several choices:
N-terminal Flag-tagged; C-terminal Flag-tagged; N-terminal 6xHis-Flag-tagged; N-terminal 6xHis-tagged and C-terminal Flag-tagged; N-terminal 10xHis-tagged and C-terminal Flag-tagged; N-terminal Flag-Myc-tagged and C-terminal Flag-Myc-tagged, etc. Pls check with the customer which kind of Flag-tag he/she needs.
Regarding the HA tag, generally we will link the N-terminal 10xHis-HA-tagged, if the customer has some other demand, pls feedback to us.

靶點詳情

  • 功能：
    Critical for B-cell development.
  • 基因功能參考文獻：
    1. Hhydrophobic residues from the lambda5-UR is crucial for the interaction with GAL1 and for pre-BCR clustering. PMID: 23124203
  • 相關疾病：
    Agammaglobulinemia 2, autosomal recessive (AGM2)
  • 亞細胞定位：
    Endoplasmic reticulum. Secreted.
  • 組織特異性：
    Expressed only in pre-B-cells and a special B-cell line (which is surface Ig negative).
  • 數據庫鏈接：

    HGNC: 5870

    OMIM: 146770

    KEGG: hsa:3543

    STRING: 9606.ENSP00000329312

    UniGene: Hs.348935



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