Recombinant Human Homeobox protein Hox-B4 (HOXB4)

中文名稱：

人HOXB4重組蛋白
貨號：

CSB-YP010664HU
規(guī)格：

￥1500
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產(chǎn)品詳情
產(chǎn)品評價
相關(guān)產(chǎn)品
靶點詳情

產(chǎn)品詳情

純度：

Greater than 90% as determined by SDS-PAGE.
基因名：

HOXB4
Uniprot No.：

P17483
別名：

Homeo box 2F; Homeo box B4; Homeobox 2F; Homeobox B4; Homeobox protein Hox B4; Homeobox protein Hox-2.6; Homeobox protein Hox-2F; Homeobox protein Hox-B4; Homeobox protein HoxB4; HOX 2; Hox 2.6; Hox 2F; HOX B4; HOX2; Hox2.6; Hox2F; HOXB 4; hoxb4; HXB4_HUMAN
種屬：

Homo sapiens (Human)
蛋白長度：

Full Length
來源：

Yeast
分子量：

29.6kDa
表達區(qū)域：

1-251aa
氨基酸序列

MAMSSFLINSNYVDPKFPPCEEYSQSDYLPSDHSPGYYAGGQRRESSFQPEAGFGRRAACTVQRYAACRDPGPPPPPPPPPPPPPPPGLSPRAPAPPPAGALLPEPGQRCEAVSSSPPPPPCAQNPLHPSPSHSACKEPVVYPWMRKVHVSTVNPNYAGGEPKRSRTAYTRQQVLELEKEFHYNRYLTRRRRVEIAHALCLSERQIKIWFQNRRMKWKKDHKLPNTKIRSGGAAGSAGGPPGRPNGGPRAL
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標(biāo)簽：

N-terminal 6xHis-tagged
產(chǎn)品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

產(chǎn)品評價

靶點詳情

功能：

Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.
基因功能參考文獻：
1. Data suggests that an impaired capacity of eutopic and ectopic endometrial tissue to upregulate levels of HOXB4 during the proliferative phase may play a role in the pathogenesis of endometriosis and that further downregulation of HOXB4 may enhance ectopic implant invasiveness. PMID: 28969513
2. Authors observed that induced expression of HOXB4 in the UW473 cell line significantly reduced in vitro cell proliferation and migration capability of UW473 cells with no effect on the in vivo tumorigenicity. PMID: 29039487
3. Study revealed that HOXB4 was highly expressed in ovarian cancer cells. HOXB4 silencing enhanced the cytotoxic effect of Taxol and DDP by downregulating ABC transporters via inhibition of the PI3K/Akt signaling pathway. These results for the first time elucidated the critical roles and molecular basis of HOXB4 underlying drug resistance in ovarian cancer cells. PMID: 29660518
4. HOXB4 knockout led to downregulation of P-glycoprotein, multidrug resistance-associated protein 1 and breast cancer resistance protein expression and PI3K/Akt signaling activity, suggesting that repression of HOXB4 might be a key point to reverse Multidrug resistance of K562/ADM cells. PMID: 27779650
5. This study provides novel evidences of the mechanisms integrating Notch and TNF-alpha signaling in the transcriptional induction of GATA3 and HOXB4. PMID: 27251160
6. Increased expression of HOXB4 in human embryonic stem cells enhances the production of hematopoietic Progenitors but does not effect the maturation of red blood cells. PMID: 27352929
7. The analysis of HOX expression in primary mesothelioma tumors indicated that these cells could also be sensitive to the disruption of HOX activity by HXR9, and that the expression of HOXB4 is strongly associated with overall survival PMID: 26867567
8. showed that OAC1 treatment led to OCT4-mediated upregulation of HOXB4 PMID: 26202933
9. a crucial regulator of NK lytic function PMID: 24810639
10. Epigenetic analysis revealed that increased promoter methylation of HOXB4 correlates with decreased expression of its transcript in oral cancer cell lines. HOXB4 may work as an epigenetic biomarker gene. PMID: 24859765
11. Our study demonstrates for the first time the regulation of HOXB4 by miR-23a PMID: 23630040
12. our results outline the effects of HOXB4 in combination with stromal cells in the development of NK cells from embryonic stem cells. PMID: 22761810
13. GATA-2 directly regulates HOXB4 expression in hematopoietic stem cells, which may play an important role in the development and/or progression of aplastic anemia. PMID: 23028422
14. HOXB4-positivity as an independent predictor of overall survival of acute myeloid leukemia patients PMID: 22664110
15. Expressions of ABCB1, BMI-1, HOXB4 were not detected in the patients with non-malignant hematologic diseases, but were higher in de novo acute leukemia patients and lower in patients in complete remission. PMID: 22040961
16. Comparative transcriptome analysis of CD34(+) cells subjected or not to HOXB4 or HOXC4 demonstrated that both homeoproteins regulate the same set of genes, some of which encode key hematopoietic factors and signaling molecules. PMID: 22298821
17. cytomegalovirus infection markedly down-regulated HOXB4 expression which affected proliferation and differentiation of erythroid and lymphocyte progenitor cells. PMID: 22402911
18. Down-regulation of mitochondria and lysosomal genes by HoxB4 plays a role in the impaired lymphoid lineage development from embryonic stem cells-derived hematopoietic stem cells. PMID: 22438249
19. Hox B4 protein is present in the precursor lesions as CC cells, suggesting that Hox B4 could be a protein related to the neoplastic state (non-differentiated cells) of human cervical epithelium. PMID: 22120585
20. These results indicate that transient, but not constitutive, HoxB4 expression is sufficient to augment the hematopoietic differentiation of embryonic stem and induced pluripotent stem cells. PMID: 22000550
21. The demethylation of CpG island in the promoter region of HOXB4 gene may be correlated with the high expression of HOXB4 gene in CD34(+) cells, while the promoter methylation of HOXB4 gene may be associated with HOXB4 gene silencing in PBMCs. PMID: 19549386
22. these data suggest that increased HoxB4 enhanced early megakaryocytic development in human TF1 cells and CD34 positively-selected cord blood cells primarily by upregulating TpoR and Fli-1 expression and downregulating c-Myb expression. PMID: 20599537
23. overexpression of HOXB4 in human ESC did not improve the generation of CD34(+) hematopoietic cells via co-culture methodology PMID: 19878058
24. may play a role in the regulation of cellular proliferation/adhesion in developing fetal human epidermis and in hyperproliferation conditions, including cancers, in adult epidermis PMID: 11984874
25. Retrovirally mediated expression of HOXB4 rapidly triggers an increase in the number of stem cells without alternating the balance of lymphomyeloid reconstitution, suggesting that HOXB4 does not affect control of end-cell output. PMID: 12130496
26. In NOD/SCID mice HOXB4-overexpressing cord blood CD34+ cells had a selective growth advantage in vivo. However, high HOXB4 expression substantially impaired myeloerythroid differentiation & B-cell output. PMID: 12406897
27. NF-Y is a developmentally regulated inducer of the HOXB4 gene in hematopoietic cells. PMID: 12791656
28. The growth promoting effects of HOXB4 are critically dependent on HOXB4 expression levels and that this can result in important species-specific differences in potency. PMID: 17510218
29. The protein transduction domain-Hoxb4protein can be used with other recombinant proteins to efficiently generate transplantable hemaat4opoietic stem cells from human embryonic stem cells. PMID: 17784829
30. contribution of HOXB4 in the maintenance of the intrinsic lymphomyeloid differentiation potential of defined hematopoietic progenitor cell subsets PMID: 17962697
31. overexpression of HoxB4 in differentiating hESCs increases hematopoietic colony formation and hematopoietic cell formation in vitro, but does not affect in vivo repopulation in adult mice hosts PMID: 18511880
32. These data suggest that HoxB4-induced effects on human embryonic stem cell-derived hematopoietic stem cells are concentration-dependent during in vitro development and reduce proliferation of other cell types in vitro and in vivo. PMID: 18617691
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亞細(xì)胞定位：

Nucleus.
蛋白家族：

Antp homeobox family, Deformed subfamily
數(shù)據(jù)庫鏈接：

HGNC: 5115

OMIM: 142965

KEGG: hsa:3214

STRING: 9606.ENSP00000328928

UniGene: Hs.664706