Recombinant Human Histone-lysine N-methyltransferase MECOM (MECOM EVI1 MDS1 PRDM3)
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中文名稱:
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貨號:CSB-YP2290HU
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規格:
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來源:Yeast
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其他:
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中文名稱:
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貨號:CSB-EP2290HU
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規格:
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來源:E.coli
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其他:
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中文名稱:
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貨號:CSB-EP2290HU-B
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規格:
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來源:E.coli
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共軛:Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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其他:
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中文名稱:
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貨號:CSB-BP2290HU
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規格:
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來源:Baculovirus
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其他:
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中文名稱:
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貨號:CSB-MP2290HU
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規格:
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來源:Mammalian cell
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其他:
產品詳情
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純度:>85% (SDS-PAGE)
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基因名:
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Uniprot No.:
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別名:Histone-lysine N-methyltransferase MECOM; EC 2.1.1.367; Ecotropic virus integration site 1 protein homolog; EVI-1; MDS1 and EVI1 complex locus protein; Myelodysplasia syndrome 1 protein; Myelodysplasia syndrome-associated protein 1; MECOM EVI1 MDS1 PRDM3
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種屬:Homo sapiens (Human)
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蛋白標簽:Tag?type?will?be?determined?during?the?manufacturing?process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially. -
產品提供形式:Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand. -
復溶:We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
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儲存條件:Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
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保質期:The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. -
貨期:Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
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注意事項:Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
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Datasheet :Please contact us to get it.
相關產品
靶點詳情
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功能:Functions as a transcriptional regulator binding to DNA sequences in the promoter region of target genes and regulating positively or negatively their expression. Oncogene which plays a role in development, cell proliferation and differentiation. May also play a role in apoptosis through regulation of the JNK and TGF-beta signaling. Involved in hematopoiesis.; Displays histone methyltransferase activity and monomethylates 'Lys-9' of histone H3 (H3K9me1) in vitro. Probably catalyzes the monomethylation of free histone H3 in the cytoplasm which is then transported to the nucleus and incorporated into nucleosomes where SUV39H methyltransferases use it as a substrate to catalyze histone H3 'Lys-9' trimethylation. Likely to be one of the primary histone methyltransferases along with PRDM16 that direct cytoplasmic H3K9me1 methylation.
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基因功能參考文獻:
- High EVI1 expression predicts poor outcome in Acute myeloid leukemia with intermediate cytogenetic risk in patients receiving chemotherapy. PMID: 29408852
- The relationship between EVI1 and microRNAs in human malignancies. [review] PMID: 29879503
- Fisher's exact SubID was used to reveal EVI1 as a transcriptional regulator of INPP4B in AML; a finding which was validated in vitro. Next, we used CoxPH SubID to conduct a pan-cancer analysis of INPP4B's prognostic significance PMID: 29415082
- These findings highlight a novel mechanism for hepatitis B virus X-induced hepatocarcinogenesis through transcription factor EVI1 and its target lncRNAs PMID: 26967394
- Findings represent the first global genome-wide study of EVI1 DNA binding associated with whole transcriptome expression analysis. Results reveal several important genes with an ETS-like binding motif, is involved in terminal myeloid differentiation, cell cycle regulation and apoptosis PMID: 23826213
- The expression of EVI1 and SOX9 is associated with stem cell-like and metastasis signatures, and their depletion impairs the metastatic potential of breast cancer cells. These results establish the mechanistic link between resistance to mTOR inhibition and cancer metastatic potential, thus enhancing our understanding of mTOR targeting failure PMID: 27991928
- EVI1 contributes to PCa progression by regulating different oncogenic functions. EVI1 regulates the PCa stem cell compartment responsible for disease initiation and also development of CRPC. PMID: 27617580
- oncogenic potential for EVI1 in modulating genomic stability PMID: 26234679
- EVI1 acts as a regulator of its own expression, highlighting the complex regulation of EVI1, and open new directions to better understand the mechanisms of EVI1 overexpressing leukemias. PMID: 28391050
- EVI1 overexpression alters cellular metabolism. EVI1 promotes CKMT1 expression by repressing the myeloid differentiation regulator RUNX1. PMID: 28191887
- EVI1 transcription is directly regulated by LEF1/beta-catenin complex in myeloid blast crisis of chronic myeloid leukemia. Loss of p53 function as a key regulator for beta-catenin-EVI1 in myeloid blast crisis of chronic myeloid leukemia. PMID: 27908728
- we demonstrated that miR-22 promoted monocyte/macrophage differentiation, and MECOM (EVI1) mRNA is a direct target of miR-22 and MECOM (EVI1) functions as a negative regulator in the differentiation.The miR-22-mediated MECOM degradation increased c-Jun but decreased GATA2 expression, which results in increased interaction between c-Jun and PU.1 PMID: 27617961
- Results establish the oncogenic contributions of EVI1 in ER- and HER2-negative subsets of breast cancer. PMID: 28209621
- MECOM gene harbors both somatic frameshift mutations. PMID: 27620344
- Isochromosome 3q, especially MECOM abnormality, could play a key role in Persistent polyclonal binucleated B-cell lymphocytosis. PMID: 26935937
- MECOM deletion is associated with congenital thrombocytopenia. PMID: 26554871
- involvement of MECOM in patients with acute myeloid leukemia and myelodysplastic syndrome with various 3q abnormalities PMID: 26815134
- RUNX1-Evi-1 may promote proliferation and apoptosis resistance of primitive hematopoietic cell, and inhibit the differentiation of myeloid cells with the synergy of different pathways and factors. PMID: 26674644
- EVI1 overexpression has been revealed as an important independent adverse prognostic marker in adult acute myeloid leukemia and defines distinct risk categories in 11q23-rearranged AML. PMID: 26729571
- Data suggest that alteration of ecotropic viral integration site-1 (EVI1) expression was more profound in acute lymphoblastic leukemia (ALL) than in acute myeloid leukemia (AML). PMID: 24828867
- Mutations in MECOM cause radioulnar synostosis with amegakaryocytic thrombocytopenia. PMID: 26581901
- A RT-qPCR assay was used to quantify EVI1 expression covering the different splice variants in bone marrow and blood in AML. EVI1 expression levels significantly decreased between diagnostic and post-induction samples. PMID: 26467814
- Low EVI1 expression is associated with chronic lymphocytic leukemia. PMID: 25936528
- Our data reveal MS4A3 as a novel direct target of EVI1 in human myeloid cells, and show that its repression plays a role in EVI1 mediated tumor aggressiveness. PMID: 25886616
- EVI1 antagonizes TGF-beta-mediated growth inhibition of hepatocllular carcinoma cells. PMID: 25959919
- There is breakpoint heterogeneity in (2;3)(p15-23;q26) translocations involving EVI1 in myeloid hemopathies found in four unrelated patients. PMID: 25486926
- EVI1 modulated the all-trans retinoic acid response of several dozens of genes. PMID: 25486480
- EVI1 and MEL1 are homolog genes whose transcriptional activations by chromosomal translocations have roles in Japanese pediatric acute myeloid leukemia PMID: 25567132
- We found that EVI1 and HIC1 colocalize in the nucleus, and their interaction is mediated by the amino terminal zinc finger binding domain of EVI1 PMID: 24907396
- Findings suggest that the EVI-1 rs6774494 G > A single nucleotide polymorphism targeted by miRNA-206/133b may contribute to the pathogenesis of breast cancer. PMID: 24935473
- Evi1-high leukemic cells themselves possess the superior potential to Evi1-low cells in oncogenic self-renewal. PMID: 24747972
- EVI1-positive children with acute myeloid leukemia have a poor short-term prognosis PMID: 24568904
- Co-expression of EVI1 and BCR/ABL fusion gene can be found in childhood CML and ALL. PMID: 24856998
- Authors identify a mechanism whereby a GATA2 distal hematopoietic enhancer (G2DHE or -77-kb enhancer) is brought into close proximity to the EVI1 gene in inv(3)(q21;q26) inversions, leading to leukemogenesis. PMID: 24703906
- 3q inversion and translocation reposition a distal GATA2 enhancer to ectopically activate EVI1 and simultaneously confer GATA2 functional haploinsufficiency, previously identified as the cause of sporadic familial acute myeloid leukemia/myelodysplastic syndrome. PMID: 24703711
- we found that EVI1 upregulated KRAS expression through suppression of miR-96 in pancreatic cancer cells PMID: 23752186
- EVI1 binds to DeltaNp63 promoter element directly and down regulates its expression, inducing the expression of p21 in colon carcinoma cells that do not express p53, that eventually delay cell cycle progression at G0/G1 phase. PMID: 23665236
- Phosphorylation of the leukemic oncoprotein EVI1 on serine 196 modulates DNA binding, transcriptional repression and transforming ability. PMID: 23776681
- The MECOM core promoter GA-repeats are also conserved in numerous species, of which human has the longest repeat and complexity. PMID: 24055488
- Expression of EVI1 and EVI1(Del427-515) (but not EVI1(Del190-515)) in OVCAR8 ovarian cancer cells increased cyclin E1 LMW expression and cell cycle progression. PMID: 23517670
- PR-domain protein ME has an essential role in mixed-lineage leukemia (MLL) fusion protein (MFP) leukemia PMID: 24021671
- EVI1 oncoprotein interacts with a large and complex network of proteins and integrates signals through protein phosphorylation. PMID: 23858473
- we identified a new locus associated with OF (rs784288 in the MECOM gene) that showed genome-wide significance (p=3.59x10(-8); OR 1.39). RNA interference revealed that a MECOM knockdown suppresses osteoclastogenesis. PMID: 23349225
- human myeloid cell line constitutively overexpressing EVI1 was partially resistant to etoposide and daunorubicin PMID: 23457546
- elevated EVI1 expression or in AML/MDS patients with chromosome 7 abnormalities. PMID: 22887804
- Cell cycle analysis demonstrated EVI1's anti-proliferative effect to be strictly dose-dependent with accumulation of cells in G0/G1 PMID: 23212151
- EVI1 binds to the promoter of miR-9-3, leading to DNA hypermethylation of the promoter and repression of miR-9. PMID: 23509296
- Overexpression of Evi1 oncoprotein is identified as a novel mechanism by which a subset of human human colorectal cancer cells may escape TGF-beta regulation. PMID: 22161860
- EVI1 is expressed in human hematopoietic progenitor cells, but is down-regulated during differentiation PMID: 23155256
- Deregulated expression of EVI1 defines a subset of MLL-rearranged acute myeloid leukemias. PMID: 23008312
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相關疾病:Radioulnar synostosis with amegakaryocytic thrombocytopenia 2 (RUSAT2)
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亞細胞定位:Nucleus. Nucleus speckle. Cytoplasm.
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