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  4. Recombinant Human Ethanolamine kinase 1 (ETNK1)

Recombinant Human Ethanolamine kinase 1 (ETNK1)

  • 中文名稱：
    人ETNK1重組蛋白
  • 貨號：
    CSB-YP007850HU
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    人ETNK1重組蛋白
  • 貨號：
    CSB-EP007850HU
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    人ETNK1重組蛋白
  • 貨號：
    CSB-EP007850HU-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    人ETNK1重組蛋白
  • 貨號：
    CSB-BP007850HU
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    人ETNK1重組蛋白
  • 貨號：
    CSB-MP007850HU
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    ETNK1
  • Uniprot No.：
  • 別名：
    1110061E11Rik; 4930555L11Rik; AI195356; AI841245; C80956; D6Ertd3e; EKI 1; EKI; EKI1; EKI1_HUMAN; Ethanolamine kinase 1; ETNK1; Nbla10396; Putative protein product of Nbla10396
  • 種屬：
    Homo sapiens (Human)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-452
  • 氨基酸序列
    MLCGRPRSSS DNRNFLRERA GLSSAAVQTR IGNSAASRRS PAARPPVPAP PALPRGRPGT EGSTSLSAPA VLVVAVAVVV VVVSAVAWAM ANYIHVPPGS PEVPKLNVTV QDQEEHRCRE GALSLLQHLR PHWDPQEVTL QLFTDGITNK LIGCYVGNTM EDVVLVRIYG NKTELLVDRD EEVKSFRVLQ AHGCAPQLYC TFNNGLCYEF IQGEALDPKH VCNPAIFRLI ARQLAKIHAI HAHNGWIPKS NLWLKMGKYF SLIPTGFADE DINKRFLSDI PSSQILQEEM TWMKEILSNL GSPVVLCHND LLCKNIIYNE KQGDVQFIDY EYSGYNYLAY DIGNHFNEFA GVSDVDYSLY PDRELQSQWL RAYLEAYKEF KGFGTEVTEK EVEILFIQVN QFALASHFFW GLWALIQAKY STIEFDFLGY AIVRFNQYFK MKPEVTALKV PE
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

問答及客戶評論

■ 常見問題解答
Q:

CSB-EP007850HU; CSB-YP007850HU; CSB-BP007850HU; CSB-MP007850HU Recombinant Human Ethanolamine kinase 1 (ETNK1)
Please can you provide information for all available sizes of all available expression hosts?
Has there been any customer feedback regarding activity of this protein?

A:
Very nice to receive your inquiry. Recombinant Human Ethanolamine kinase 1(ETNK1)
CSB-YP007850HU >> Yeast
CSB-EP007850HU >> E.coli
CSB-BP007850HU >> Baculovirus
CSB-MP007850HU >> Mammalian cell
Expression Region: 1-452aa; Full length.
Tag information:EP, YP, BP, MP: Tag type will be determined during the manufacturing process.
The expected tag for each expression system is list as follows:
YP: N-terminal 6xHis-tagged; EP, BP, MP: N-terminal 10xHis-tagged and C-terminal Myc-tagged.
Sequence:

MLCGRPRSSSDNRNFLRERAGLSSAAVQTRIGNSAASRRSPAARPPVPAPPALPRGRPGTEGSTSLSAPAVLVVAVAVVVVVVSAVAWAMANYIHVPPGSPEVPKLNVTVQDQEEHRCREGALSLLQHLRPHWDPQEVTLQLFTDGITNKLIGCYVGNTMEDVVLVRIYGNKTELLVDRDEEVKSFRVLQAHGCAPQLYCTFNNGLCYEFIQGEALDPKHVCNPAIFRLIARQLAKIHAIHAHNGWIPKSNLWLKMGKYFSLIPTGFADEDINKRFLSDIPSSQILQEEMTWMKEILSNLGSPVVLCHNDLLCKNIIYNEKQGDVQFIDYEYSGYNYLAYDIGNHFNEFAGVSDVDYSLYPDRELQSQWLRAYLEAYKEFKGFGTEVTEKEVEILFIQVNQFALASHFFWGLWALIQAKYSTIEFDFLGYAIVRFNQYFKMKPEVTALKVPE


Regarding the activity, sorry, as all of them are semi-customized proteins that we haven't expressed before, we are not sure about the activity and can't 100% guarantee its activity.
As what we provide is the full length and the proteins will be purified under very mild condition, they are very likely to be active, we recommend you to purchase a small size to have a try. You can consider the Eukaryotic expression system. Generally, the more advanced expression system is the protein expressed with, the more likely to be active.

靶點詳情

  • 功能：
    Highly specific for ethanolamine phosphorylation. May be a rate-controlling step in phosphatidylethanolamine biosynthesis.
  • 基因功能參考文獻：
    1. we identified novel somatic missense ETNK1 mutations that were most frequent in systemic mastocytosis with eosinophilia and chronic myelomonocytic leukemia PMID: 25615281
    2. Recurrent ETNK1 mutations are associated with chronic myeloid leukemia. PMID: 25343957
    3. DAD-R, SOX5, and EKI1 map within region of chromosome 12p whose duplication is related to reduced apoptosis in human testicular seminomas. PMID: 11912161
  • 亞細胞定位：
    Cytoplasm.
  • 蛋白家族：
    Choline/ethanolamine kinase family
  • 組織特異性：
    Expressed in kidney, liver, placenta, heart, leukocyte, ovary and testis.
  • 數據庫鏈接：

    HGNC: 24649

    OMIM: 609858

    KEGG: hsa:55500

    STRING: 9606.ENSP00000266517

    UniGene: Hs.29464



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