在线日韩日本国产亚洲丨少妇伦子伦情品无吗丨欧美性猛交xxxx免费看蜜桃丨精品人妻系列无码一区二区三区丨亚洲精品无码不卡在线播放

Your Good Partner in Biology Research

  1. 首頁
  2. 蛋白
  3. 重組蛋白
  4. Recombinant Human E3 ubiquitin-protein ligase pellino homolog 1 (PELI1)

Recombinant Human E3 ubiquitin-protein ligase pellino homolog 1 (PELI1)

  • 中文名稱：
    人PELI1重組蛋白
  • 貨號：
    CSB-YP856932HU
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    人PELI1重組蛋白
  • 貨號：
    CSB-EP856932HU
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    人PELI1重組蛋白
  • 貨號：
    CSB-EP856932HU-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    人PELI1重組蛋白
  • 貨號：
    CSB-BP856932HU
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    人PELI1重組蛋白
  • 貨號：
    CSB-MP856932HU
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    PELI1
  • Uniprot No.：
  • 別名：
    E3 ubiquitin-protein ligase pellino homolog 1; PELI1; PELI1_HUMAN; Pellino E3 ubiquitin protein ligase 1; Pellino homolog 1; Pellino protein; Pellino-1; Pellino-related intracellular-signaling molecule; Pellino1; PRISM; Protein pellino homolog 1
  • 種屬：
    Homo sapiens (Human)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-418
  • 氨基酸序列
    MFSPDQENHP SKAPVKYGEL IVLGYNGSLP NGDRGRRKSR FALFKRPKAN GVKPSTVHIA CTPQAAKAIS NKDQHSISYT LSRAQTVVVE YTHDSNTDMF QIGRSTESPI DFVVTDTVPG SQSNSDTQSV QSTISRFACR IICERNPPFT ARIYAAGFDS SKNIFLGEKA AKWKTSDGQM DGLTTNGVLV MHPRNGFTED SKPGIWREIS VCGNVFSLRE TRSAQQRGKM VEIETNQLQD GSLIDLCGAT LLWRTAEGLS HTPTVKHLEA LRQEINAARP QCPVGFNTLA FPSMKRKDVV DEKQPWVYLN CGHVHGYHNW GNKEERDGKD RECPMCRSVG PYVPLWLGCE AGFYVDAGPP THAFSPCGHV CSEKTTAYWS QIPLPHGTHT FHAACPFCAH QLAGEQGYIR LIFQGPLD
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

問答及客戶評論

■ 常見問題解答
Q:

Regarding your proteins CSB-EP847618HU & CSB-EP856932HU, could you please advise on the sequencing and the expected tag information for all 4 expression hosts?

Also, we want to know about the composition of the buffer - is it 50% glycerol by volume? Or by molarity, or MW, etc.? We are interested in 0.1mg and 0.5mg Baculovirus, and are wondering what the total volumes for these sizes would most likely be.

Thanks so much!

A:
Thanks for your inquiry.
Recombinant Human E3 ubiquitin-protein ligase pellino homolog 1(PELI1)
CSB-YP856932HU >> Yeast
CSB-EP856932HU >> E.coli
CSB-BP856932HU >> Baculovirus
CSB-MP856932HU >> Mammalian cell
Expression Region: 1-418aa; Full length.
Tag information:EP, YP, BP, MP: Tag type will be determined during the manufacturing process.
The expected tag for each expression system is list as follows:
YP: N-terminal 6xHis-tagged; EP, BP, MP: N-terminal 10xHis-tagged and C-terminal Myc-tagged.
Target Protein Sequence:

MFSPDQENHPSKAPVKYGELIVLGYNGSLPNGDRGRRKSRFALFKRPKANGVKPSTVHIACTPQAAKAISNKDQHSISYTLSRAQTVVVEYTHDSNTDMFQIGRSTESPIDFVVTDTVPGSQSNSDTQSVQSTISRFACRIICERNPPFTARIYAAGFDSSKNIFLGEKAAKWKTSDGQMDGLTTNGVLVMHPRNGFTEDSKPGIWREISVCGNVFSLRETRSAQQRGKMVEIETNQLQDGSLIDLCGATLLWRTAEGLSHTPTVKHLEALRQEINAARPQCPVGFNTLAFPSMKRKDVVDEKQPWVYLNCGHVHGYHNWGNKEERDGKDRECPMCRSVGPYVPLWLGCEAGFYVDAGPPTHAFSPCGHVCSEKTTAYWSQIPLPHGTHTFHAACPFCAHQLAGEQGYIRLIFQGPLD


The default buffer is Tris-based buffer, PH=8.0, 50% glycerol.
If you have some special requirement for the buffer, pls let us know in advance.
The 50% glycerol is calculated by volume, 50% refers to the final concentration of glycerol, of course, you can reduce the final concentration of glycerol if you need,
such as the final concentration of 10% glycerol, or if you don't want glycerol, we can also provide lyophilized powder (we will add 6% trehalose during the lyophilization),
the lead time will be extended for 3 working days for lyophilization.
The quotations for 0.1mg and 0.5mg Baculovirus and lead time are provided upon you request.
The concentration of BP protein is generally 0.1-0.5mg/ml after adding glycerol, for example, if the concentration is 0.5mg/ml and order quantity is 100ug, then the final volume would be 200ul,
if the order quantity is 500ug, then the final volume would be 1ml. If you have some requirements for the shipping format or package size, pls let us know in advance.

靶點詳情

  • 功能：
    E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. Involved in the TLR and IL-1 signaling pathways via interaction with the complex containing IRAK kinases and TRAF6. Mediates 'Lys-63'-linked polyubiquitination of IRAK1 allowing subsequent NF-kappa-B activation. Mediates 'Lys-48'-linked polyubiquitination of RIPK3 leading to its subsequent proteasome-dependent degradation; preferentially recognizes and mediates the degradation of the 'Thr-182' phosphorylated form of RIPK3. Negatively regulates necroptosis by reducing RIPK3 expression. Mediates 'Lys-63'-linked ubiquitination of RIPK1.
  • 基因功能參考文獻：
    1. Overexpression of Peli1 inhibits noncanonical NF-kappaB activation and alleviates lupus-like disease. PELI1 levels negatively correlate with disease severity in SLE patients. PMID: 29555915
    2. Pellino-1 may be critically important for cell survival. PMID: 29860626
    3. This study demonstrates Pellino 1 (PELI1) as an important modulator that exerts opposite regulatory functions on apoptosis and necroptosis, two distinct forms of regulated cell death mechanisms. PMID: 29078411
    4. The study demonstrates that cytosolic Pellino-1-mediated K63-linked ubiquitination of IRF5 in M1 macrophages regulates glucose intolerance in obesity, suggesting a cytosolic mediator function of Pellino-1 in TLR4/IFN-gamma receptor-IRF5 axis during M1 polarization. PMID: 28746869
    5. Pellino 1 expression acts as an inhibitory signal of the homeostatic regulation of mitotic cell cycle and checkpoint, and thus contributes to the initiation and progression of neoplastic chromosome aneuploidy. PMID: 28410192
    6. Pellino-1 overexpression activated PI3K/Akt and ERK signaling pathways and elicited an epithelial-mesenchymal transition (EMT) phenotype of lung adenocarcinoma cells. Pellino-1-mediated EMT was demonstrated through morphology, the upregulation of Vimentin, Slug and Snail expression and the downregulation of E-cadherin and beta-catenin expression. PMID: 28009353
    7. Results indicate that Pellino-1 contributes to lung oncogenesis through the overexpression of inhibitor of apoptosis protein 2 (cIAP2) and promotion of cell survival and chemoresistance. PMID: 27248820
    8. Our results suggest that PELI1 might participate in B-cell maturation or oncogenic activation of aggressive B-cell lymphomas, both during and after germinal center stages PMID: 27469333
    9. The combination of low Pellino3 levels together with high and inducible Pellino1 expression may be an important determinant of the degree of inflammation triggered upon Toll-like receptor 2 engagement by Helicobacter pylori and/or its components, contributing to Helicobacter pylori-associated pathogenesis by directing the incoming signal toward an NF-kB-mediated proinflammatory response. PMID: 27302665
    10. Our observations suggested that Peli-1 gene polymorphism rs329498 might contribute to SLE susceptibility in Chinese Han Population. PMID: 27018966
    11. The study results in the Chinese Han population showed that PELI1 is a member of a constellation of genetic factors that may contribute to the pathogenesis of systemic lupus erythematosus. PMID: 25712248
    12. Increased PELI1 expression and subsequent induction of BCL6 promotes lymphomagenesis. PMID: 25295537
    13. Peptide PEL1 derived from the interleukin-1 receptor-associated kinase (IRAK)1-binding motif reveals a distinct phosphothreonine peptide binding preference. PMID: 25027698
    14. GWAS study found two new SNPs associated with nickel dermatitis; SNPs are located in the NTN4 and PELI1 genes PMID: 23921680
    15. Pellino1 interacts with the transcription factor Deformed Epidermal Autoregulatory Factor 1 (DEAF1). PMID: 23846693
    16. incomplete KD, IVIG nonresponsiveness, long febrile days, and the rs7604693 genetic variant in the PELI1 gene are major risk factors for the development of CALs PMID: 22105492
    17. role in interleukin-1-mediated signalling through interaction with interleukin-1 receptor-associated kinase 4-IRAK-tumor necrosis factor receptor-associated factor 6 complex PMID: 12496252
    18. Smad6 bound to Pellino-1 promoted TGF-beta-mediated anti-inflammatory effects. PMID: 16951688
    19. kinase-inactive IRAK proteins can associate with Pellino proteins, thus excluding the possibility that their inability to regulate Pellino degradation is due to lack of association with the Pellino proteins PMID: 17675297

    顯示更多

    收起更多

  • 蛋白家族：
    Pellino family
  • 組織特異性：
    Expressed at high levels in normal skin but decreased in keratinocytes from toxic epidermal necrolysis (TEN) patients (at protein level).
  • 數據庫鏈接：

    HGNC: 8827

    OMIM: 614797

    KEGG: hsa:57162

    STRING: 9606.ENSP00000351789

    UniGene: Hs.7886



主站蜘蛛池模板： 四虎影视永久免费观看在线| 国内精品久久久久影院蜜芽| 亚洲腹肌男啪啪网站男同| 亚洲精品gv天堂无码男同| 久久综合给合综合久久| 国产成a人亚洲精v品无码| 午夜成人无码片在线观看影院| 动漫精品中文无码卡通动漫| 久久av无码αv高潮αv喷吹| 黑人糟蹋人妻hd中文字幕| 2020最新无码国产在线观看| 国产综合一区二区三区黄页秋霞| 青青草大香焦在线综合视频| 亚洲爆乳无码一区二区三区| 国产免费人成视频在线播放播| 亚洲一卡2卡3卡4卡5卡乱码观看 | 无码高潮爽到爆的喷水视频| 中文人妻无码一区二区三区信息| 久久精品囯产精品亚洲| 亚洲人成人影院在线观看| 中文在线8资源库| 亚洲vs日韩vs欧美vs久久| 亚洲色欲色欲天天天www| 国产乱女乱子视频在线播放| 艳z门照片无码av| 亚洲另类激情综合偷自拍图片| 久久99亚洲精品久久69| 午夜视频在线瓜伦| 天天做天天爱天天综合网| 黄网站色视频免费观看| 欧美牲交a欧美牲交aⅴ| 免费拍拍拍网站| 久久人人爽人人爽人人片ⅴ| 久久精品国产亚洲欧美| 女人国产香蕉久久精品| 在线永久免费观看黄网站| 天堂资源中文最新版在线一区| 国产女人叫床高潮大片| 97伦伦午夜电影理伦片| 欧美巨大xxxx做受高清| 狠狠色丁香久久婷婷综合_中|