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  4. Recombinant Human DNA topoisomerase 3-alpha (TOP3A), partial

Recombinant Human DNA topoisomerase 3-alpha (TOP3A), partial

  • 中文名稱：
    人TOP3A重組蛋白
  • 貨號：
    CSB-YP621672HU
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    人TOP3A重組蛋白
  • 貨號：
    CSB-EP621672HU
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    人TOP3A重組蛋白
  • 貨號：
    CSB-EP621672HU-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    人TOP3A重組蛋白
  • 貨號：
    CSB-BP621672HU
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    人TOP3A重組蛋白
  • 貨號：
    CSB-MP621672HU
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    TOP3A
  • Uniprot No.：
  • 別名：
    DNA topoisomerase 3 alpha; DNA topoisomerase 3-alpha; DNA topoisomerase III alpha; TOP 3; TOP3; TOP3A; TOP3A_HUMAN; topo III-alpha; topoisomerase (DNA) III alpha; topoisomerase (DNA) III; topoisomerase III alpha; ZGRF7; zinc finger; GRF-type containing 7
  • 種屬：
    Homo sapiens (Human)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone. As an essential component of the RMI complex it is involved in chromosome separation and the processing of homologous recombination intermediates to limit DNA crossover formation in cells. Has DNA decatenation activity. It is required for mtDNA decatenation and segregation after completion of replication, in a process that does not require BLM, RMI1 and RMI2.
  • 基因功能參考文獻：
    1. Top3alpha is shown to be an essential component of the mitochondrial DNA replication machinery and as the first component of the mitochondrial DNA separation machinery. PMID: 29290614
    2. The results show that Topo IIIalpha stimulates DNA unwinding by BLM in a manner that is potentiated by RMI1-RMI2, and that the processivity of resection is reliant on the Topo IIIalpha-RMI1-RMI2 complex. PMID: 25200081
    3. hTOP3alpha interacts with p53, regulates p53 and p21 expression, and contributes to the p53-mediated tumor suppression PMID: 24526736
    4. MPS1 protein-dependent phosphorylation of RecQ-mediated genome instability protein 2 (RMI2) at serine 112 is essential for BLM-Topo III alpha-RMI1-RMI2 (BTR) protein complex function upon spindle assembly checkpoint (SAC) activation during mitosis PMID: 24108125
    5. Study found a correlation between topoisomerase IIIalpha immunoexpression and oral squamous cell carcinoma (OSCC) cancer stem cell immunophenotypes. However, results did not show a prognostic significance of topoisomerases in OSCC> PMID: 22672768
    6. TOP3A, a new partner gene fused to MLL in an adult patient with de novo acute myeloid leukaemia. PMID: 22050635
    7. human topoisomerase IIIalpha functions as a decatenase with the assistance of BLM and RMI1 to facilitate the processing of homologous recombination intermediates without crossing over as a mechanism to preserve genome integrity PMID: 20445207
    8. G-quadruplex ligand telomestatin impairs binding of topoisomerase IIIalpha to G-quadruplex-forming oligonucleotides and uncaps telomeres in ALT cells PMID: 19742304
    9. Dual localization to mitochondria and nucleus PMID: 12209014
    10. The Bloom's syndrome helicase stimulates the activity of topoisomerase IIIalpha PMID: 12433984
    11. The effects of TOP3 on telomere binding in human and S. cerevisiae cells are reported. PMID: 16546998
    12. BLM, Topo IIIalpha, and BLAP75 constitute a dissolvasome complex that processes HR intermediates to limit DNA crossover formation PMID: 16595695
    13. BLM is stably associated with TOP3A. PMID: 17728255
    14. evolutionarily conserved N-terminal third of BLAP75 mediates complex formation with BLM and Topo IIIalpha and that the DNA binding activity resides in the C-terminal third of this novel protein. PMID: 18390547
    15. Topo IIIalpha is an important telomere-associated factor, essential for telomere maintenance and chromosome stability in ALT cells, and speculate on its potential mechanistic function. PMID: 18418389
    16. Individuals carrying genetic variants of the BLM-TOP3A-RMI1 complex have an increased risk of acute myeloid leukemia/myelodysplatic syndromes, malignant melanoma, bladder and breast cancer. PMID: 19432957

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  • 亞細胞定位：
    Mitochondrion matrix.
  • 蛋白家族：
    Type IA topoisomerase family
  • 組織特異性：
    High expression is found in testis, heart, skeletal muscle and pancreas.
  • 數據庫鏈接：

    HGNC: 11992

    OMIM: 601243

    KEGG: hsa:7156

    STRING: 9606.ENSP00000321636

    UniGene: Hs.592115



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