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  4. Recombinant Human DNA-directed RNA polymerase III subunit RPC7 (POLR3G)

Recombinant Human DNA-directed RNA polymerase III subunit RPC7 (POLR3G)

  • 中文名稱：
    人POLR3G重組蛋白
  • 貨號：
    CSB-YP018349HU
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    人POLR3G重組蛋白
  • 貨號：
    CSB-EP018349HU
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    人POLR3G重組蛋白
  • 貨號：
    CSB-EP018349HU-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    人POLR3G重組蛋白
  • 貨號：
    CSB-BP018349HU
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    人POLR3G重組蛋白
  • 貨號：
    CSB-MP018349HU
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    POLR3G
  • Uniprot No.：
  • 別名：
    DNA directed RNA polymerase III 32 kDa polypeptide; DNA-directed RNA polymerase III subunit G; DNA-directed RNA polymerase III subunit RPC7; Polr3g; Polymerase (RNA) III (DNA directed) polypeptide G (32kD); RNA polymerase III 32 kDa subunit; RNA polymerase III subunit C7; RPC32; RPC7; RPC7_HUMAN
  • 種屬：
    Homo sapiens (Human)
  • 蛋白長度：
    Full length protein
  • 表達區域：
    1-223
  • 氨基酸序列
    MAGNKGRGRA AYTFNIEAVG FSKGEKLPDV VLKPPPLFPD TDYKPVPLKT GEGEEYMLAL KQELRETMKR MPYFIETPEE RQDIERYSKR YMKVYKEEWI PDWRRLPREM MPRNKCKKAG PKPKKAKDAG KGTPLTNTED VLKKMEELEK RGDGEKSDEE NEEKEGSKEK SKEGDDDDDD DAAEQEEYDE EEQEEENDYI NSYFEDGDDF GADSDDNMDE ATY
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

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靶點詳情

  • 功能：
    DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. May direct with other members of the RPC3/POLR3C-RPC6/POLR3F-RPC7/POLR3G subcomplex RNA Pol III binding to the TFIIIB-DNA complex via the interactions between TFIIIB and POLR3F. May be involved either in the recruitment and stabilization of the subcomplex within RNA polymerase III, or in stimulating catalytic functions of other subunits during initiation. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs), induce type I interferon and NF- Kappa-B through the RIG-I pathway.
  • 基因功能參考文獻：
    1. POLR3G regulates small non-coding RNAs and RNA splicing in human pluripotent stem cells. The primary function of POLR3G is in the maintenance rather than repression of transcription. PMID: 28494942
    2. Decreased levels of POLR3G result in loss of pluripotency and promote differentiation of human embryonic stem cells to all three germ layers but have no effect on cell apoptosis. PMID: 21898682
  • 亞細胞定位：
    Nucleus. Cytoplasm.
  • 蛋白家族：
    Eukaryotic RPC7 RNA polymerase subunit family
  • 組織特異性：
    Barely detectable in differentiated tissues. Expressed in embryonic stem cells and in other dividing cells, such as some tumor cell lines.
  • 數據庫鏈接：

    HGNC: 30075

    OMIM: 617456

    KEGG: hsa:10622

    STRING: 9606.ENSP00000382058

    UniGene: Hs.282387



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