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  4. Recombinant Human DNA-directed RNA polymerase II subunit RPB7 (POLR2G)

Recombinant Human DNA-directed RNA polymerase II subunit RPB7 (POLR2G)

  • 中文名稱(chēng)：
    人POLR2G重組蛋白
  • 貨號(hào)：
    CSB-YP018334HU
  • 規(guī)格：
  • 來(lái)源：
    Yeast
  • 其他：
  • 中文名稱(chēng)：
    人POLR2G重組蛋白
  • 貨號(hào)：
    CSB-EP018334HU
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 其他：
  • 中文名稱(chēng)：
    人POLR2G重組蛋白
  • 貨號(hào)：
    CSB-EP018334HU-B
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱(chēng)：
    人POLR2G重組蛋白
  • 貨號(hào)：
    CSB-BP018334HU
  • 規(guī)格：
  • 來(lái)源：
    Baculovirus
  • 其他：
  • 中文名稱(chēng)：
    人POLR2G重組蛋白
  • 貨號(hào)：
    CSB-MP018334HU
  • 規(guī)格：
  • 來(lái)源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    POLR2G
  • Uniprot No.：
  • 別名：
    DNA directed RNA polymerase II 19 kDa polypeptide ; DNA directed RNA polymerase II subunit G; DNA directed RNA polymerase II subunit RPB7; DNA-directed RNA polymerase II subunit G; DNA-directed RNA polymerase II subunit RPB7; hRPB19; hsRPB7; MGC138367; MGC138369; POLR2G; polymerase (RNA) II (DNA directed) polypeptide G; RNA polymerase II 19 kDa subunit; RNA polymerase II subunit B7; RPB19; RPB7 ; RPB7_HUMAN
  • 種屬：
    Homo sapiens (Human)
  • 蛋白長(zhǎng)度：
    Full length protein
  • 表達(dá)區(qū)域：
    1-172
  • 氨基酸序列
    MFYHISLEHE ILLHPRYFGP NLLNTVKQKL FTEVEGTCTG KYGFVIAVTT IDNIGAGVIQ PGRGFVLYPV KYKAIVFRPF KGEVVDAVVT QVNKVGLFTE IGPMSCFISR HSIPSEMEFD PNSNPPCYKT MDEDIVIQQD DEIRLKIVGT RVDKNDIFAI GSLMDDYLGL VS
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB7 is part of a subcomplex with RPB4 that binds to a pocket formed by RPB1, RPB2 and RPB6 at the base of the clamp element. The RBP4-RPB7 subcomplex seems to lock the clamp via RPB7 in the closed conformation thus preventing double-stranded DNA to enter the active site cleft. The RPB4-RPB7 subcomplex binds single-stranded DNA and RNA. Binds RNA.
  • 基因功能參考文獻(xiàn)：
    1. The function of Rpb7 interaction with rpb4 in human cells indicate that Rpb7 have gene-specific effects but are also more generally required for human cell survival. PMID: 23073835
    2. Identification of the RNA binding region of Rpb7. PMID: 16282592
    3. the emerging transcript cross-links to the Rpb7 subunit of RNA polymerase II in various complexes containing 26- to 32-nt transcripts PMID: 16327806
    4. TFIIF and Rpb7 are involved in both early and late transcriptional stages PMID: 17848138
  • 亞細(xì)胞定位：
    Nucleus.
  • 蛋白家族：
    Eukaryotic RPB7/RPC8 RNA polymerase subunit family
  • 數(shù)據(jù)庫(kù)鏈接：

    HGNC: 9194

    OMIM: 602013

    KEGG: hsa:5436

    STRING: 9606.ENSP00000301788

    UniGene: Hs.14839



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