Recombinant Human Cyclin-G-associated kinase (GAK), partial

^{In Stock}

中文名稱：

人GAK重組蛋白
貨號：

CSB-EP009190HUh2
規格：

￥1836
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

Greater than 85% as determined by SDS-PAGE.
生物活性：

Not Test
基因名：

GAK
Uniprot No.：

O14976
種屬：

Homo sapiens (Human)
蛋白長度：

Partial
來源：

E.coli
分子量：

40.8
表達區域：

40-314aa
氨基酸序列

LRVRRVLAEGGFAFVYEAQDVGSGREYALKRLLSNEEEKNRAIIQEVCFMKKLSGHPNIVQFCSAASIGKEESDTGQAEFLLLTELCKGQLVEFLKKMESRGPLSCDTVLKIFYQTCRAVQHMHRQKPPIIHRDLKVENLLLSNQGTIKLCDFGSATTISHYPDYSWSAQRRALVEEEITRNTTPMYRTPEIIDLYSNFPIGEKQDIWALGCILYLLCFRQHPFEDGAKLRIVNGKYSIPPHDTQYTVFHSLIRAMLQVNPEERLSIAEVVHQLQ
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 6xHis-tagged and C-terminal Avi-tagged
產品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

Associates with cyclin G and CDK5. Seems to act as an auxilin homolog that is involved in the uncoating of clathrin-coated vesicles by Hsc70 in non-neuronal cells. Expression oscillates slightly during the cell cycle, peaking at G1.
基因功能參考文獻：
1. These findings indicate that the abnormal mitoses seen after silencing OIP5-AS1 were caused by an untimely rise in GAK levels and suggest that OIP5-AS1 suppresses cell proliferation at least in part by reducing GAK levels. PMID: 28472763
2. that the multiple single nucleotide polymorphisms of GAK synergistically participate in the pathogenesis of sporadic Parkinson's disease through multiple pathways. PMID: 26676575
3. We have demonstrated that siRNA-mediated GAK silencing causes marked cell cycle disruption resulting in a raised sub-G1 alongside decreased G1 and G2/M cell cycle phases. Dual FBXW7-GAK inhibition increases multipolar mitoses PMID: 28829765
4. GAK gene expression is increased in Parkinson disease PMID: 27508417
5. These results suggest that the c-Src_GAK_MCM axis plays an important role in cell cycle progression through control of the DNA replication licensing system. PMID: 28135906
6. We filtered four OSCC genes including SERPINB9, SERPINE2, GAK, and HSP90B1 through the gene global prioritization score (P < 0.005). PMID: 26318431
7. This meta-analysis suggests that GAK rs1564282 C/T polymorphism is associated with increased susceptibility to Parkinson's disease--{REVIEW} PMID: 25975492
8. Data indicated that GBA and TMEM175/GAK significantly alter age at onset in PD. PMID: 25914293
9. AAK1 and GAK are critical regulators of HCV entry that function in part by activating EGFR, AP2M1, and NUMB, and as the molecular targets underlying the antiviral effect of sunitinib and erlotinib, respectively. PMID: 25653444
10. The effects of rs11248051 and rs1564282 variants of GAK, and the rs3129882 variant of HLA-DRA, were investigated in Parkinson's disease patients. PMID: 24039160
11. GAK apo structure reveals a dimeric inactive state of the catalytic domain mediated by an unusual activation segment interaction. PMID: 24438162
12. Cyclin-G-associated kinase is a binding partners of LRRK2, a candidate genes for risk for sporadic Parkinson disease, and part of a complex that promotes clearance of Golgi-derived vesicles through the autophagy-lysosome system. PMID: 24510904
13. Dominant negative mutants of IRAK4 and GAK show strong apoptotic effects in A498 cells under anoxia. PMID: 23591012
14. neither the CT, TT genotypes nor the minor allele T of single nucleotide polymorphism rs1564282 were associated with Parkinson's disease among the subjects from Taiwan and Singapore. PMID: 23826309
15. rs11248060 significantly increasThis study found that the minor alleles of GAK rpopulationss1564282 and DGKQ e the risk of PD in Han Chinese . PMID: 23618683
16. GAK as a regulator of dephosphorylation events under the control of the PP2A B'gamma subunit. PMID: 22262175
17. study demonstrated that the rs1564282 variant in GAK (PARK17) increases the risk of Parkinson's disease in Han Chinese patients from mainland China PMID: 22198721
18. cyclin G associated kinase is associated with Parkinson disease risk and suggest that cyclin G associated kinase and alpha-synuclein interact in a pathway involved in disease pathogenesis PMID: 21258085
19. GAK is a new candidate for investigation in future studies. PMID: 21058943
20. Osteosarcoma cell proliferation and survival are dependent on GAK. PMID: 20881269
21. results identify a role for GAK and clathrin in microtubule outgrowth from kinetochores/chromosomes and suggest that GAK acts through clathrin to control microtubule outgrowth around chromosomes PMID: 20237935
22. The GAK localizes in both cytoplasm and nucleus by immunostaining, ectopic expression of GFP-GAK and pull-down assays using dissected GAK fragments. PMID: 19371378
23. down-regulation of GAK results in outgrowth of cells in soft agar, raising the possibility that loss of GAK function may promote tumorigenesis. PMID: 15240878
24. GAK enhanced the androgen receptor transcriptional response even at low concentrations of androgens PMID: 16161052
25. Results describe the recruitment dynamics of GAK and auxilin to clathrin-coated pits during endocytosis. PMID: 16895969
26. Interactions between the PsiG[PDE][PsiLM]-motif sequences in GAK and the AP1-gamma-ear domain are critical for the recruitment of GAK to the trans-Golgi network and the function of GAK in lysosomal enzyme sorting. PMID: 17538018
27. GAK and CHC cooperated in the same pathway and interacted in mitosis to regulate the formation of a functional spindle. PMID: 19654208
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亞細胞定位：

Cytoplasm, perinuclear region. Golgi apparatus, trans-Golgi network. Cell junction, focal adhesion. Note=Localizes to the perinuclear area and to the trans-Golgi network. Also seen on the plasma membrane, probably at focal adhesions.
蛋白家族：

Protein kinase superfamily, Ser/Thr protein kinase family
組織特異性：

Ubiquitous. Highest in testis.
數據庫鏈接：

HGNC: 4113

OMIM: 602052

KEGG: hsa:2580

STRING: 9606.ENSP00000314499

UniGene: Hs.369607