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  4. Recombinant Human Ammonium transporter Rh type B (RHBG), partial

Recombinant Human Ammonium transporter Rh type B (RHBG), partial

  • 中文名稱：
    人RHBG重組蛋白
  • 貨號：
    CSB-YP887968HU
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    人RHBG重組蛋白
  • 貨號：
    CSB-EP887968HU
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    人RHBG重組蛋白
  • 貨號：
    CSB-EP887968HU-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    人RHBG重組蛋白
  • 貨號：
    CSB-BP887968HU
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    人RHBG重組蛋白
  • 貨號：
    CSB-MP887968HU
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    RHBG
  • Uniprot No.：
  • 別名：
    Ammonium transporter Rh type B; Rh family B glycoprotein; Rh family type B glycoprotein; Rh type B glycoprotein; RHBG; RHBG_HUMAN; Rhesus blood group B glycoprotein; Rhesus blood group family type B glycoprotein; SLC42A2
  • 種屬：
    Homo sapiens (Human)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Functions as a specific ammonium transporter.
  • 基因功能參考文獻：
    1. RHBG expression as a direct target of beta-catenin regulation. PMID: 26029888
    2. Kidney AE1 actually associates with epithelial ankyrin-G and renal ammonium transporter RhBG, which also binds ankyrin-G. PMID: 25616663
    3. Rh B Glycoprotein (Rhbg) and Rh C Glycoprotein (Rhcg) are identified as ammonia transporters. They are expressed in specific cell populations and membrane domains in distal renal epithelial cells, where they facilitate ammonia secretion. PMID: 24647713
    4. RhAG, RhBG and RhCG-exhibit significant permeability to NH3 and show for the first time that RhBG and RhCG can conduct CO2. PMID: 24077989
    5. human kidney expresses basolateral RhBG protein in connecting segment, type A intercalated cells, and non-A, non-B cells PMID: 23324176
    6. Electric current measurements and intracellular pHi determinations suggest that RhBG acts as an electroneutral NH4+-H+ exchanger. PMID: 15284342
    7. RhBG requires a tyrosine-based signal and ankyrin-G for basolateral targeting and membrane PMID: 15611082
    8. RhBG and RhCG facilitate ammonium movement across the plasma membrane; these results favor the hypothesis that these Rh glycoproteins, together with their homolog RhAG, constitute a family of NH3 channels in mammalian cells. PMID: 15929723
    9. Phosphorylation and ankyrin-G binding of the C-terminal domain regulate targeting and function of the ammonium transporter RhBG PMID: 18635543
    10. This study concludes that under normal conditions, RhCG is the major putative ammonia transporter expressed in the human kidney and RhBG is not expressed at detectable levels. PMID: 19357182
    11. Rhbg and Rhcg may contribute to bronchial epithelial cell ammonia metabolism and suggest that they do not contribute to pulmonary carbon dioxide transport. PMID: 19429772

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  • 亞細胞定位：
    Basolateral cell membrane; Multi-pass membrane protein. Cytoplasmic vesicle membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Ammonium transporter (TC 2.A.49) family, Rh subfamily
  • 組織特異性：
    Specifically expressed in kidney. Also detected in liver and ovary.
  • 數據庫鏈接：

    HGNC: 14572

    OMIM: 607079

    KEGG: hsa:57127

    STRING: 9606.ENSP00000357229

    UniGene: Hs.131835



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