Recombinant Human A-kinase anchor protein 12 (AKAP12 AKAP250)
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中文名稱:
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貨號(hào):CSB-YP896230HU
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規(guī)格:
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來源:Yeast
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其他:
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中文名稱:
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貨號(hào):CSB-EP896230HU
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規(guī)格:
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來源:E.coli
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其他:
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中文名稱:
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貨號(hào):CSB-EP896230HU-B
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規(guī)格:
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來源:E.coli
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共軛:Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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其他:
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中文名稱:
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貨號(hào):CSB-BP896230HU
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規(guī)格:
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來源:Baculovirus
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其他:
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中文名稱:
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貨號(hào):CSB-MP896230HU
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規(guī)格:
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來源:Mammalian cell
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其他:
產(chǎn)品詳情
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純度:>85% (SDS-PAGE)
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基因名:AKA12
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Uniprot No.:
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別名:A-kinase anchor protein 12; AKAP-12; A-kinase anchor protein 250 kDa; AKAP 250; Gravin; Myasthenia gravis autoantigen; AKAP12 AKAP250
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種屬:Homo sapiens (Human)
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蛋白標(biāo)簽:Tag?type?will?be?determined?during?the?manufacturing?process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially. -
產(chǎn)品提供形式:Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand. -
復(fù)溶:We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
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儲(chǔ)存條件:Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
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保質(zhì)期:The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. -
貨期:Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
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注意事項(xiàng):Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
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Datasheet :Please contact us to get it.
相關(guān)產(chǎn)品
靶點(diǎn)詳情
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功能:Anchoring protein that mediates the subcellular compartmentation of protein kinase A (PKA) and protein kinase C (PKC).
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基因功能參考文獻(xiàn):
- Low AKAP12 expression is associated with high-grade meningiomas. PMID: 29391485
- There is a positive association between the expression of AKAP12 and Semaphorin 3F in prostate cancer, suggesting that the activation of Semaphorin 3F by AKAP12 may be involved in prostate cancer progression and metastasis. PMID: 28698137
- A critical role for AKAP12 as an UV-inducible scaffold for PKA-mediated ATR phosphorylation. PMID: 27683220
- the AKAP12a promoter shows high DNA methylation levels in juvenile myelomonocytic leukemia, which go along with reduced AKAP12a mRNA expression levels PMID: 26891149
- MiR-103 regulates hepatocellular carcinoma growth by targeting AKAP12 PMID: 26646106
- results suggest that the association between the 6q25.1 locus and breast cancer risk may be mediated through single nucleotide polymorphisms that regulate expressions of the AKAP12 gene. PMID: 26645718
- The authors propose that Gravin-mediated recruitment of Aurora A and Plk1 to the mother (oldest) spindle pole contributes to the fidelity of symmetric cell division. PMID: 26406118
- The SSeCKS, a major Lipopolysaccharide (LPS) response protein, has been involved in regulating astrocyte activation via production of proinflammatory factor in CNS inflammation. PMID: 26428665
- Elevated expression of AKAP12 is characteristic of paclitaxel-resistant high-grade serous ovarian cancer cells, and a prognostic and predictive marker for poor overall survival in SOC patients. PMID: 25748058
- The enrichment of activated Cdc42 in SSeCKS-null leading edge filopodia correlated with recruitment of the Cdc42-specific guanine nucleotide exchange factor, Frabin. PMID: 25356636
- AKAP12 scaffolding protein mediates meningeal reconstruction after central nervous system injury PMID: 25229625
- PKA compartmentalization via AKAP220 and AKAP12 contributes to endothelial barrier regulation. PMID: 25188285
- This study showed that AKAP12,CAMK2D and a molecular pathway(cyclic amp)association to outcome of depressive during citalopram treatment. PMID: 24986638
- AKAP12 variant 2 hypoxic induction shifts PKA-mediated protein phosphorylation to enhance migration and metastasis of melanoma cells PMID: 25792458
- AKAP12 expression was positive in 82.2% of tumors in a cohort with colorectal adenocarcinoma. AKAP12 expression was unrelated to p53 or Bcl-2 expression. PMID: 24870731
- Receptor-mediated Ca2+ and PKC signaling triggers the loss of cortical PKA compartmentalization through the redistribution of gravin. PMID: 23838009
- High AKAP12 expression is associated with colorectal cancer. PMID: 24065476
- AKAP12 is differentially expressed in human astrocytomas showing high expression in pilocytic but low expression in diffuse astrocytomas of all WHO-grades. further, epigenetic mechanisms are involved in silencing AKAP12 in diffuse astrocytomas PMID: 24042196
- Gravin gene expression is decreased in acute myeloid leukemia and correlates with poor prognosis. PMID: 23543478
- Data suggest a model in which SSeCKS suppresses oncogenic motility by sequestering Src to caveolin-rich lipid rafts, thereby disengaging Src from FAK-associated adhesion and signaling complexes. PMID: 22710722
- demonstrated for the first time that AKAP12 tumor/angiogenesis suppressor gene is an epigenetic target of HDAC7 PMID: 22584896
- It defines a role for Gravin as a temporal organizer of phosphorylation-dependent protein-protein interactions during mitosis. PMID: 23063527
- The results demonstrate that AKAP12 may play an important role in tumor growth suppression and the survival of human colorectal cancer. PMID: 21918680
- PKC-mediated remodeling of the actin cytoskeleton is likely regulated by the ability of SSeCKS to control PKC signaling and activity through a direct scaffolding function PMID: 21903576
- AKAP12 is involved in the regulation of endothelial cell migration through the inhibitory regulation of MMP-9 expression in tumor cells. PMID: 21461577
- AKAP12 promoter methylation is a frequent event in human prostate cancer. PMID: 21310466
- SERPINB5 and AKAP12 may have a role in increased metastasis in pancreatic ductal adenocarcinoma PMID: 20939879
- Data show that AKAP12 methylation represents a potential molecular biomarker for predicting the malignancy of colorectal cancer. PMID: 20364105
- AKAP12alpha promoter methylation is associated with lung cancer. PMID: 21115911
- In addition to genetic alterations, epigenetic mechanisms are responsible for the reduction of the tumor suppressor gene AKAP12 in human hepatocarcinogenesis. PMID: 20979053
- Findings suggest that SSeCKS suppresses metastatic motility by disengaging activated Src and then inhibiting the PKC-Raf/MEK/ERK pathways controlling matrix metalloproteinase-2 expression and podosome formation. PMID: 20018890
- gravin provides a dynamic platform for the localization for kinases during neuronal development PMID: 12857743
- AKAP250 acts as a scaffold that binds protein kinase A (PKA), protein kinase C and protein phosphatases, associating reversibly with the beta(2)-adrenergic receptor. PMID: 14657015
- AKAP12A may function as an important negative regulator of the survival pathway in human gastric cancer. PMID: 15258566
- AKAP12alpha belongs to a novel class of atypical SRF-dependent target genes PMID: 15590635
- AKAP12 localization is regulated by a hierarchy of targeting domains and the localization of AKAP12-assembled signaling complexes may be dynamically regulated PMID: 15923193
- Results suggest that gravin maintains a signaling complex that includes protein kinase A and phosphodiesterase 4D. PMID: 16642035
- Membrane binding of gravin, and thus function, can be reversed by calcium/calmodulin, which binds to the membrane and causes translocation of gravin from membrane to cytoplasm. PMID: 16762919
- We conclude that LPA-dependent increased level of cAMP in senescent human diploid fibroblasts is associated with increases in Gravin levels resulting in its increased binding with and activation of calcium-dependent PKC alpha/beta and AC4/6. PMID: 17081159
- The Src-binding peptide-(1-51) of gravin behaves as a dominant-negative for AKAP gravin regulation of beta2-adrenergic receptor resensitization/recycling PMID: 17200117
- Results suggest that AKAP12 may play an important role in tumor growth suppression by inducing apoptosis with the regulation of multiple molecules in the cell cycle progression. PMID: 17442483
- AKAP12 may induce BRB formation through antiangiogenesis and barriergenesis in developing human eye and defects in this mechanism can lead to loss of tight junction proteins and contribute to development of retinal pathologies such as retinoblastoma. PMID: 17442832
- The Gravin expression was found to be decreased in samples of acute leukaemia and was associated with an inferior overall survival. PMID: 17577780
- Hypermethylation of the AKAP12 promoter is associated esophageal carcinoma. PMID: 18199717
- AKAP12 in astrocytes induces barrier functions in human endothelial cells through protein kinase Czeta. PMID: 18397319
- These findings implicate AKAP12 in the regulation of cytokinesis progression, and suggest a novel role for AKAP12 tumor suppressor. PMID: 18554502
- Results suggest that AKAP12A may activate SREBP-2 by increasing cholesterol efflux, and is a novel regulator of cellular cholesterol metabolism. PMID: 18579430
- AKAP12 docks to the beta2-adrenergic receptor in response to agonist stimulation, later internalizes with agonist-induced receptors, and finally dissociates from the recycled, cell membrane-bound receptor. PMID: 18950703
- Data show that PKC activation resulted in redistribution of gravin and PKA constructs to the same subcellular site, and suggest that this response to PKC activity may mediate PKC dependent control of PKA activity. PMID: 19210988
- Data revealed that six polymorphisms of F10, PITRM1, PCSK2, JPH3, MYO7B, and AKAP12 were related (P<0.05) to the prevalence of chronic kidney disease. PMID: 19724895
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亞細(xì)胞定位:Cytoplasm, cell cortex. Cytoplasm, cytoskeleton. Membrane; Lipid-anchor. Note=May be part of the cortical cytoskeleton.
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組織特異性:Expressed in endothelial cells, cultured fibroblasts and osteosarcoma, but not in platelets, leukocytes, monocytic cell lines or peripherical blood cells.
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