在线日韩日本国产亚洲丨少妇伦子伦情品无吗丨欧美性猛交xxxx免费看蜜桃丨精品人妻系列无码一区二区三区丨亚洲精品无码不卡在线播放

Your Good Partner in Biology Research

  1. 首頁
  2. 蛋白
  3. 重組蛋白
  4. Recombinant Human 3-oxo-5-beta-steroid 4-dehydrogenase (AKR1D1)

Recombinant Human 3-oxo-5-beta-steroid 4-dehydrogenase (AKR1D1)

  • 中文名稱：
    Recombinant Human 3-oxo-5-beta-steroid 4-dehydrogenase(AKR1D1)
  • 貨號：
    CSB-YP001548HU
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Human 3-oxo-5-beta-steroid 4-dehydrogenase(AKR1D1)
  • 貨號：
    CSB-EP001548HU
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    Recombinant Human 3-oxo-5-beta-steroid 4-dehydrogenase(AKR1D1)
  • 貨號：
    CSB-EP001548HU-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Human 3-oxo-5-beta-steroid 4-dehydrogenase(AKR1D1)
  • 貨號：
    CSB-BP001548HU
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Human 3-oxo-5-beta-steroid 4-dehydrogenase(AKR1D1)
  • 貨號：
    CSB-MP001548HU
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 別名：
    3-oxo-5-beta-steroid 4-dehydrogenase; 3o5bred ; AK1D1_HUMAN; AKR1D1; aldo keto reductase family 1 member D1 (delta 4 3 ketosteroid 5 beta reductase); Aldo keto reductase family 1 member D1; Aldo-keto reductase family 1 member D1; CBAS2 ; Delta(4) 3 ketosteroid 5 beta reductase; Delta(4) 3 oxosteroid 5 beta reductase; Delta(4)-3-ketosteroid 5-beta-reductase; Delta(4)-3-oxosteroid 5-beta-reductase; SRD5B1; steroid 5 beta reductase beta polypeptide 1 (3 oxo 5 beta steroid delta 4 dehydrogenase beta 1); steroid 5 beta reductase
  • 種屬：
    Homo sapiens (Human)
  • 蛋白長度：
    Full length protein
  • 表達區(qū)域：
    1-326
  • 氨基酸序列
    MDLSAASHRI PLSDGNSIPI IGLGTYSEPK STPKGACATS VKVAIDTGYR HIDGAYIYQN EHEVGEAIRE KIAEGKVRRE DIFYCGKLWA TNHVPEMVRP TLERTLRVLQ LDYVDLYIIE VPMAFKPGDE IYPRDENGKW LYHKSNLCAT WEAMEACKDA GLVKSLGVSN FNRRQLELIL NKPGLKHKPV SNQVECHPYF TQPKLLKFCQ QHDIVITAYS PLGTSRNPIW VNVSSPPLLK DALLNSLGKR YNKTAAQIVL RFNIQRGVVV IPKSFNLERI KENFQIFDFS LTEEEMKDIE ALNKNVRFVE LLMWRDHPEY PFHDEY
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Catalyzes the stereospecific NADPH-dependent reduction of the C4-C5 double bond of bile acid intermediates and steroid hormones carrying a delta(4)-3-one structure to yield an A/B cis-ring junction. This cis-configuration is crucial for bile acid biosynthesis and plays important roles in steroid metabolism. Capable of reducing a broad range of delta-(4)-3-ketosteroids from C18 (such as, 17beta-hydroxyestr-4-en-3-one) to C27 (such as, 7alpha-hydroxycholest-4-en-3-one).
  • 基因功能參考文獻：
    1. infant proved to be a compound heterozygote of the AKR1D1 variants c.579+2delT and c.853C>T(p.Q285X), two novel mutations originated from his mother and father, respectively PMID: 28697823
    2. Impaired NADPH binding and hydride transfer is the molecular basis for bile acid deficiency in patients with the P133R mutation in AKR1D1. PMID: 26418565
    3. When different steroid substrates were used in single turnover experiments with AKR1D1. PMID: 25500266
    4. AKR1D1 generates all 5beta-dihydrosteroids in the C19-C27 steroid series. PMID: 24513054
    5. Despite having high kchem values with steroid hormones, the kinetic control of AKR1D1 is consistent with the enzyme catalysing the slowest step in the catabolic sequence of steroid hormone transformation in the liver. PMID: 24894951
    6. Studies indicate that mutations in aldo-keto reductase family 1 (AKR1) enzymes AKR1C1 and AKR1C4 are responsible for sexual development dysgenesis and mutations in AKR1D1 are causative in bile-acid deficiency. PMID: 24189185
    7. Novel homozygous frameshift mutations in the AKR1D1 gene and in the SKIV2L gene were found in a family with severe infantile liver disease. PMID: 23679950
    8. Consistent with AKR1D1's putative role as a driver of the P450 subnetwork, the AKR1D1 3'-UTR SNP was significantly associated with increased hepatic mRNA expression of multiple P450s PMID: 23704699
    9. These studies show how a single point mutation in AKR1D1 can introduce HSD activity with unexpected configurational and stereochemical preference. PMID: 22437839
    10. all five mutations identified in patients with functional bile acid deficiency strongly affected AKR1D1 enzyme functionality and therefore may be causal for this disease PMID: 21185810
    11. determined the substrate specificity of homogeneous human recombinant AKR1D1 using C18, C19, C21, and C27 Delta(4)-ketosteroids and assessed the pH-rate dependence of the enzyme. PMID: 21255593
    12. analysis of disease-related 5beta-reductase (AKR1D1) mutations reveals their potential to cause bile acid deficiency PMID: 20522910
    13. In placenta and myometrium, relative expression decreased significantly in association with labour, by about two-fold and 10-fold, respectively. These data are consistent with a possible role for 5betaDHP in the onset of spontaneous human labour. PMID: 16123077
    14. analysis of human liver Delta4-3-ketosteroid 5beta-reductase (AKR1D1) crystal structure and implications for substrate binding and catalysis PMID: 18407998
    15. The structures of an AKR1D1-NADP(+) binary complex, and AKR1D1-NADP(+)-cortisone, AKR1D1-NADP(+)-progesterone and AKR1D1-NADP(+)-testosterone ternary complexes at high resolutions, is reported. PMID: 18848863
    16. Structure determination of human AKR1C4 and homology modelling of AKR1D1 followed by docking experiments were used to explore active site geometries. PMID: 19013211
    17. Delta4-3-ketosteroid 5beta-reductase (AKR1D1) has an alternative binding site responsible for substrate inhibition PMID: 19075558
    18. SRD5B1 gene analysis needed for the accurate diagnosis of primary 3-oxo-Delta4-steroid 5beta-reductase deficiency. PMID: 19175828

    顯示更多

    收起更多

  • 相關(guān)疾病：
    Congenital bile acid synthesis defect 2 (CBAS2)
  • 亞細胞定位：
    Cytoplasm.
  • 蛋白家族：
    Aldo/keto reductase family
  • 組織特異性：
    Highly expressed in liver. Expressed in testis and weakly in colon.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 388

    OMIM: 235555

    KEGG: hsa:6718

    STRING: 9606.ENSP00000242375

    UniGene: Hs.201667



主站蜘蛛池模板： 国产熟妇午夜精品aaa| 少妇做爰免费视频了| 亚洲最大无码中文字幕| 2019最新久久久视频精品| 天堂网www天堂资源网| 国产精品久久久爽爽爽麻豆色哟哟| 精品av无码国产一区二区| 亚洲成a人片在线观看无码下载| 人妻丰满熟妇a无码区| 国产一区精选播放022| 四虎av永久在线精品免费观看| 国产成人精品无码专区| 五月丁香综合缴情六月小说| 韩国19禁主播深夜福利视频| 国产男小鲜肉同志免费| 无码av波多野结衣| 日韩精品无码成人专区| 久久久久国产精品人妻电影| 巨人精品福利官方导航| 日韩乱码人妻无码中文字幕视频 | 国产午夜无码片免费| 亚洲欧美日韩国产自偷| 色老板精品视频在线观看| 国内大量揄拍人妻精品視頻| 中文字幕日韩人妻不卡一区| 天堂а在线中文在线新版| 特黄特色的大片观看免费视频| 久久精品国产亚洲欧美成人| 成人精品一区二区三区在线观看 | 亚洲欧洲专线一区| 亚洲中文有码字幕青青| 国产午夜精品久久久久免费视| 亚洲欧美丝袜精品久久| 又湿又黄裸乳漫画无遮挡网站| 精品无码国模私拍视频| 欧美一进一出抽搐大尺度视频| 亚洲另类无码专区丝袜| 欧美亚洲日本国产其他| 久久99精品久久久久子伦 | 亚洲 欧美 成人 自拍 高清| 在线播放亚洲人成电影|