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  4. Recombinant Horse Cholesterol side-chain cleavage enzyme, mitochondrial (CYP11A1)

Recombinant Horse Cholesterol side-chain cleavage enzyme, mitochondrial (CYP11A1)

  • 中文名稱：
    Recombinant Horse Cholesterol side-chain cleavage enzyme, mitochondrial(CYP11A1)
  • 貨號：
    CSB-YP006389HO
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Horse Cholesterol side-chain cleavage enzyme, mitochondrial(CYP11A1)
  • 貨號：
    CSB-EP006389HO
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    Recombinant Horse Cholesterol side-chain cleavage enzyme, mitochondrial(CYP11A1)
  • 貨號：
    CSB-EP006389HO-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Horse Cholesterol side-chain cleavage enzyme, mitochondrial(CYP11A1)
  • 貨號：
    CSB-BP006389HO
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Horse Cholesterol side-chain cleavage enzyme, mitochondrial(CYP11A1)
  • 貨號：
    CSB-MP006389HO
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 別名：
    CYP11A1Cholesterol side-chain cleavage enzyme; mitochondrial; EC 1.14.15.6; CYPXIA1; Cholesterol desmolase; Cytochrome P450 11A1; Cytochrome P450(scc)
  • 種屬：
    Equus caballus (Horse)
  • 蛋白長度：
    Full Length of Mature Protein
  • 表達區域：
    40-520
  • 氨基酸序列
    M SSHSPRPFKE IPSPGDNGWI NLYHFWREKG PKKLHYHHFQ NFQKYGPIYR EKLGNVESVY IVDPEDVALL FKFEGPHPER FLIPPWTAYH QYFQKPVGVL FKSSDAWKKD RLALNPEVMA LESIKNFIPL LDPVSQDFVS LLHRRMEQQG SGKFSGPIIE DLFRFAFESI TNVIFGERQG MLDEIVDPEA QRFIDAVYKM FHTSVPMLSL PPDLFRLFRT KTWRDHVAAW DTVFSKAEQY TEKFYQDLKQ KRHFDSYPGI FYRLLASNKL PFKDIQANVT EMLAGGVDTT SMSLQWHLYE IARNLRVQEM LREEVLAARR QAQGDTSTMV QMVPLLKASI KETLRLHPIA VTLQRYPQND LVIRDYMIPA KTLVQVSIYT MGQDPTFFSN PRRFDPTRWL DKNKDLTHFR NLGFGWGVRQ CLGRRIAELE MTLFLIHILE NFRVEIQHLN DVDSTFGLIL IPEKPISFTF WPITRAPPQA
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    A cytochrome P450 monooxygenase that catalyzes the side-chain hydroxylation and cleavage of cholesterol to pregnenolone, the precursor of most steroid hormones. Catalyzes three sequential oxidation reactions of cholesterol, namely the hydroxylation at C22 followed with the hydroxylation at C20 to yield 20R,22R-hydroxycholesterol that is further cleaved between C20 and C22 to yield the C21-steroid pregnenolone and 4-methylpentanal. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate and reducing the second into a water molecule. Two electrons are provided by NADPH via a two-protein mitochondrial transfer system comprising flavoprotein FDXR (adrenodoxin/ferredoxin reductase) and nonheme iron-sulfur protein FDX1 or FDX2 (adrenodoxin/ferredoxin).
  • 亞細胞定位：
    Mitochondrion inner membrane; Peripheral membrane protein.
  • 蛋白家族：
    Cytochrome P450 family
  • 數據庫鏈接：


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