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  4. Recombinant Glutamine synthetase (glnA)

Recombinant Glutamine synthetase (glnA)

  • 中文名稱：
    蠟樣芽胞桿菌glnA重組蛋白
  • 貨號：
    CSB-YP009553BQJ
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    蠟樣芽胞桿菌glnA重組蛋白
  • 貨號：
    CSB-EP009553BQJ
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    蠟樣芽胞桿菌glnA重組蛋白
  • 貨號：
    CSB-EP009553BQJ-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    蠟樣芽胞桿菌glnA重組蛋白
  • 貨號：
    CSB-BP009553BQJ
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    蠟樣芽胞桿菌glnA重組蛋白
  • 貨號：
    CSB-MP009553BQJ
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    glnA
  • Uniprot No.：
  • 別名：
    glnAGlutamine synthetase; GS; EC 6.3.1.2; Glutamate--ammonia ligase; Glutamine synthetase I alpha; GSI alpha
  • 種屬：
    Bacillus cereus
  • 蛋白長度：
    Full Length of Mature Protein
  • 表達區域：
    2-444
  • 氨基酸序列
    ARYTKEDIF RLAKEENVKY IRLQFTDLLG VIKNVEIPVS QLTKALDNKM MFDGSSIEGF VRIEESDMYL YPDLDTWVIF PWTAEKGKVA RLICDIYNAD GTPFEGDPRN NLKRVLKEME ALGFSDFNLG PEPEFFLFKV DEKGNPTLEL NDNGGYFDLA PMDLGENCRR DIVLELEEMG FEIEASHHEV APGQHEIDFK YANAIRSCDD IQTFKLVVKT IARKHGLHAT FMPKPLYGVN GSGMHCNLSL FKNGENVFYD QNGDLQLSDD ARHFIAGILK HAPAFTAVAN PTVNSYKRLV PGYEAPCYVA WSAQNRSPLV RIPASRGIST RVEVRSVDPA ANPYLVMATL LAAGLDGIKN KLTPPAAVDR NIYVMTKEER EEAGIVDLPA TLAQALVTLQ SNEVISNALG DHLLEHFIEA KEFEWDIFRT QVHQWERDQY MSLY
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Glutamine synthetase (GS) is an unusual multitasking protein that functions as an enzyme, a transcription coregulator, and a chaperone in ammonium assimilation and in the regulation of genes involved in nitrogen metabolism. It catalyzes the ATP-dependent biosynthesis of glutamine from glutamate and ammonia. Feedback-inhibited GlnA also interacts with and regulates the activity of the transcriptional regulator TnrA. During nitrogen limitation, TnrA is in its DNA-binding active state and turns on the transcription of genes required for nitrogen assimilation. Under conditions of nitrogen excess, feedback-inhibited GlnA forms a stable complex with TnrA, which inhibits its DNA-binding activity. In contrast, feedback-inhibited GlnA acts as a chaperone to stabilize the DNA-binding activity of GlnR, which represses the transcription of nitrogen assimilation genes.
  • 亞細胞定位：
    Cytoplasm.
  • 蛋白家族：
    Glutamine synthetase family


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