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  4. Recombinant Gloeobacter violaceus Proton-gated ion channel (glvI), partial

Recombinant Gloeobacter violaceus Proton-gated ion channel (glvI), partial

  • 中文名稱：
    紫葡萄糖桿菌glvI重組蛋白
  • 貨號：
    CSB-YP742273GCI1
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    紫葡萄糖桿菌glvI重組蛋白
  • 貨號：
    CSB-EP742273GCI1
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    紫葡萄糖桿菌glvI重組蛋白
  • 貨號：
    CSB-EP742273GCI1-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    紫葡萄糖桿菌glvI重組蛋白
  • 貨號：
    CSB-BP742273GCI1
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    紫葡萄糖桿菌glvI重組蛋白
  • 貨號：
    CSB-MP742273GCI1
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    glvI
  • Uniprot No.：
  • 別名：
    glvI; glr4197; Proton-gated ion channel; GLIC; Ligand-gated ion channel; LGIC
  • 種屬：
    Gloeobacter violaceus (strain PCC 7421)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Cationic channel with similar permeabilities for Na(+) and K(+), that is activated by an increase of the proton concentration on the extracellular side. Displays no permeability for chloride ions. Shows slow kinetics of activation, no desensitization and a single channel conductance of 8 pS. Might contribute to adaptation to external pH change.
  • 基因功能參考文獻：
    1. Data suggest that neurosteroids bind to two sites per GLIC subunit, both within the transmembrane domain; one site is an intrasubunit site, and the other is located in the interface between subunits. The neurosteroids ligands used in this study were derivatives of 3alpha-hydroxypregnane and tetrahydrodeoxycorticosterone. PMID: 29301936
    2. Here we delineate protein motions underlying Gloeobacter violaceus ligand-gated ion channel gating in a membrane environment and report the interface conformation in the closed and the desensitized states. PMID: 24338475
    3. X-ray structure of a bacterial ligand-gated ion channel with ketamine bound to an intersubunit cavity that partially overlaps with the homologous antagonist-binding site. PMID: 22958642
    4. The overall findings show that His-235 in the transmembrane region of GLIC is a novel proton binding site linked to channel activation. PMID: 22084238
  • 亞細胞定位：
    Cell inner membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Ligand-gated ion channel (TC 1.A.9) family
  • 數據庫鏈接：

    KEGG: gvi:glr4197

    STRING: 251221.glr4197



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