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Recombinant Escherichia coli UvrABC system protein A (uvrA), partial

  • 中文名稱:
    大腸桿菌uvrA重組蛋白
  • 貨號:
    CSB-YP363832ENV
  • 規格:
  • 來源:
    Yeast
  • 其他:
  • 中文名稱:
    大腸桿菌uvrA重組蛋白
  • 貨號:
    CSB-EP363832ENV
  • 規格:
  • 來源:
    E.coli
  • 其他:
  • 中文名稱:
    大腸桿菌uvrA重組蛋白
  • 貨號:
    CSB-EP363832ENV-B
  • 規格:
  • 來源:
    E.coli
  • 共軛:
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他:
  • 中文名稱:
    大腸桿菌uvrA重組蛋白
  • 貨號:
    CSB-BP363832ENV
  • 規格:
  • 來源:
    Baculovirus
  • 其他:
  • 中文名稱:
    大腸桿菌uvrA重組蛋白
  • 貨號:
    CSB-MP363832ENV
  • 規格:
  • 來源:
    Mammalian cell
  • 其他:

產品詳情

  • 純度:
    >85% (SDS-PAGE)
  • 基因名:
    uvrA
  • Uniprot No.:
  • 別名:
    uvrA; dinE; b4058; JW4019; UvrABC system protein A; UvrA protein; Excinuclease ABC subunit A
  • 種屬:
    Escherichia coli (strain K12)
  • 蛋白長度:
    Partial
  • 蛋白標簽:
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式:
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet :
    Please contact us to get it.

產品評價

靶點詳情

  • 功能:
    The UvrABC repair system catalyzes the recognition and processing of DNA lesions. UvrA is an ATPase and a DNA-binding protein. A damage recognition complex composed of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. When the presence of a lesion has been verified by UvrB, the UvrA molecules dissociate.
  • 基因功能參考文獻:
    1. The movement of beta-hairpin in the third zinc-binding module of UvrA (Zn3hp) is required for damage-specific binding, UvrB loading and ATPase activities of UvrA. The conformational change of Zn3hp is controlled by ATP hydrolysis at the distal nucleotide binding site. A bi-phasic damage inspection model of UvrA was proposed in which movement of the Zn3hp plays a key role in damage recognition. PMID: 28209516
    2. Data demonstrate that the C-terminal zinc finger of UvrA is required for regulation of damage-specific DNA binding. PMID: 16829526
    3. UvrA is induced after UV irradiation. UvrA levels decrease principally as a result of ClpXP-dependent protein degradation. PMID: 19183285
  • 亞細胞定位:
    Cytoplasm.
  • 蛋白家族:
    ABC transporter superfamily, UvrA family
  • 數據庫鏈接:

    KEGG: ecj:JW4019

    STRING: 316407.85676810



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