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  4. Recombinant Escherichia coli Translation initiation factor IF-2 (infB), partial

Recombinant Escherichia coli Translation initiation factor IF-2 (infB), partial

  • 中文名稱：
    大腸桿菌infB重組蛋白
  • 貨號：
    CSB-YP015164ENV
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    大腸桿菌infB重組蛋白
  • 貨號：
    CSB-EP015164ENV
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    大腸桿菌infB重組蛋白
  • 貨號：
    CSB-EP015164ENV-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    大腸桿菌infB重組蛋白
  • 貨號：
    CSB-BP015164ENV
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    大腸桿菌infB重組蛋白
  • 貨號：
    CSB-MP015164ENV
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    infB
  • Uniprot No.：
  • 別名：
    infB; gicD; ssyG; b3168; JW3137Translation initiation factor IF-2
  • 種屬：
    Escherichia coli (strain K12)
  • 蛋白長度：
    Partial
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    One of the essential components for the initiation of protein synthesis. May protect N-formylmethionyl-tRNA(fMet) from spontaneous hydrolysis. Promotes N-formylmethionyl-tRNA(fMet) binding to the 30S pre-initiation complex (PIC). Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex. Upon addition of the 50S ribosomal subunit, IF-1, IF-2 and IF-3 are released leaving the mature 70S translation initiation complex.
  • 基因功能參考文獻(xiàn)：
    1. IF2 and unique features of initiator tRNA(fMet) help establish the translational reading frame. PMID: 28914580
    2. MD simulations of IF2 docked onto the L12-CTD pinpointed the exact interacting partners-all of which were positively charged on L12 and negatively charged on IF2, connected by salt bridges. PMID: 29686090
    3. The structural transitions of IF2 C-terminal upon GDPNP binding and following nucleotide hydrolysis support the concept of cofactor-dependent conformational switching rather than the classical model for GTPase activity. PMID: 21988058
    4. Rapid docking of 30S with 50S subunits in initiation of translation depends on switching 30S subunit-bound IF2 from its inactive to active form. PMID: 21151095
    5. The authors provide evidence that mutant IF2 specifically interacts with rpsL31 in cold, leading to a bacteriostatic effect on host cells. PMID: 19716413
    6. IF2alpha and IF2beta display almost indistinguishable properties, whereas IF2DeltaN, displays functional activities having properties and requirements distinctly different from those of the intact molecule. PMID: 16935296
    7. Data show that translational initiation factor 2 (IF2) binds ppGpp at the same site and with similar affinity as GTP, and suggest that GTP and the alarmone ppGpp can be considered two alternative physiologically relevant IF2 ligands. PMID: 16968770
    8. Overexpression of IF2 or increasing the affinity of mutant initiator tRNA for IF2 enhanced re-initiation efficiency, suggesting that IF2 is required for efficient re-initiation. PMID: 18221266
    9. Data show that initiation factor 2 ameliorated the growth and polysome defects of the delta yjeQ strain. PMID: 18223068

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  • 亞細(xì)胞定位：
    Cytoplasm.
  • 蛋白家族：
    TRAFAC class translation factor GTPase superfamily, Classic translation factor GTPase family, IF-2 subfamily
  • 數(shù)據(jù)庫鏈接：


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