在线日韩日本国产亚洲丨少妇伦子伦情品无吗丨欧美性猛交xxxx免费看蜜桃丨精品人妻系列无码一区二区三区丨亚洲精品无码不卡在线播放

Your Good Partner in Biology Research

  1. 首頁
  2. 蛋白
  3. 重組蛋白
  4. Recombinant Escherichia coli Toxin YoeB (yoeB)

Recombinant Escherichia coli Toxin YoeB (yoeB)

  • 中文名稱：
    大腸桿菌yoeB重組蛋白
  • 貨號：
    CSB-YP301653ENV
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    大腸桿菌yoeB重組蛋白
  • 貨號：
    CSB-EP301653ENV
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    大腸桿菌yoeB重組蛋白
  • 貨號：
    CSB-EP301653ENV-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    大腸桿菌yoeB重組蛋白
  • 貨號：
    CSB-BP301653ENV
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    大腸桿菌yoeB重組蛋白
  • 貨號：
    CSB-MP301653ENV
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    yoeB
  • Uniprot No.：
  • 別名：
    yoeB; b4539; JW5331; Toxin YoeB; EC 3.1.-.-; Putative endoribonuclease YoeB; Putative mRNA interferase Yoeb
  • 種屬：
    Escherichia coli (strain K12)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-84
  • 氨基酸序列
    MKLIWSEESW DDYLYWQETD KRIVKKINEL IKDTRRTPFE GKGKPEPLKH NLSGFWSRRI TEEHRLVYAV TDDSLLIAAC RYHY
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Toxic component of a type II toxin-antitoxin (TA) system. Its mode of function is controversial; it has been proposed to be an mRNA interferase but also an inhibitor of translation initiation. When overproduced in wild-type cells, inhibits bacterial growth and translation by cleavage of mRNA molecules while it has a weak effect on colony forming ability. Overproduction of Lon protease specifically activates YoeB-dependent mRNA cleavage, leading to lethality. YefM binds to the promoter region of the yefM-yeoB operon to repress transcription, YeoB acts as a corepressor.; Shown in vitro to be an mRNA interferase that requires translation for substrate cleavage; if the mRNA is mutated so as to not be translatable it is no longer cleaved. Cleavage only occurs within translated regions. Has RNase activity and preferentially cleaves at the 3'-end of purine ribonucleotides.; Also shown in vitro to be a translation initiation blocker. Binds to the 70S ribosome and 50S ribosomal subunit; binding is inhibited by hygromycin A and tetracycline, both of which bind to the 30S subunit in the A site. Thus YoeB is located at the interface between 50S and 30S ribosomes and interacts with the A site where it cleaves mRNA, blocking translation initiation.
  • 基因功能參考文獻：
    1. The authors propose that heat-activated YoeB may serve a quality control function, facilitating the recycling of stalled translation complexes through ribosome rescue pathways. PMID: 26147890
    2. YoeB acts as a repression enhancer of the yefM-yoeB cassette. PMID: 17170003
    3. Model mRNAs carrying frameshift mutations exhibited a YoeB-mediated cleavage pattern consistent with the reading frameshift thus giving strong evidence that YoeB cleavage specificity was determined by the translational reading frame. PMID: 18854355
    4. YoeB corepressor greatly enhances the YefM-operator interaction. PMID: 19028895
    5. VapC overproduction leads to Lon-dependent trans-activation of YoeB, in a way that leads to preferential YoeB-mediated cleavage at mRNA stop-codons. PMID: 19400780

    顯示更多

    收起更多

  • 蛋白家族：
    YoeB family
  • 數據庫鏈接：


主站蜘蛛池模板： 18黄暴禁片在线观看| 成年日韩片av在线网站| 久久精品aⅴ无码中文字字幕不卡| 在线天堂免费观看.www| 国产亚洲精品在av| 天天av天天翘天天综合网| 国产精品久久久久久无码| 午夜伦yy44880影院| 亚州性无码不卡免费视频| 日本中文字幕亚洲乱码| 欧美人妻日韩精品| 国产午夜福利片| 奇米777国产在线视频| 国产午夜av秒播在线观看| 国模冰莲自慰肥美胞极品人体图 | 人人妻人人澡人人爽人人精品av| 国产精品美女乱子伦高| 乱码精品一区二区三区| 欧美精品国产综合久久| 国产一区二区三区成人欧美日韩在线观看 | 久久综合av色老头免费观看| 日韩免费视频| 男女超爽视频免费播放| 99精品丰满人妻无码a片| av无码欧洲亚洲电影网| julia无码中文字幕一区| 日韩一区二区三区北条麻妃| 极品粉嫩福利午夜在线播放| 米奇7777狠狠狠狠视频影院 | 五级黄高潮片90分钟视频| 无码av免费一区二区三区a片| 成人午夜福利免费体验区| 亚洲乱亚洲乱妇50p| 日本道色综合久久影院| 精品偷拍被偷拍在线观看| 国产吃奶在线观看| 日本一本二本三区免费| 国产精品视频一区二区三区无码 | 久久精品动漫一区二区三区| 亚洲欧美日韩中字视频三区| 2020国产精品午夜福利在线观看|