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  4. Recombinant Escherichia coli Sulfoxide reductase catalytic subunit YedY (yedY)

Recombinant Escherichia coli Sulfoxide reductase catalytic subunit YedY (yedY)

  • 中文名稱：
    大腸桿菌msrP重組蛋白
  • 貨號：
    CSB-YP301197ENV
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    大腸桿菌msrP重組蛋白
  • 貨號：
    CSB-EP301197ENV
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    大腸桿菌msrP重組蛋白
  • 貨號：
    CSB-EP301197ENV-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    大腸桿菌msrP重組蛋白
  • 貨號：
    CSB-BP301197ENV
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    大腸桿菌msrP重組蛋白
  • 貨號：
    CSB-MP301197ENV
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    msrP
  • Uniprot No.：
  • 別名：
    msrP; yedY; b1971; JW1954; Protein-methionine-sulfoxide reductase catalytic subunit MsrP; EC 1.8.5.-
  • 種屬：
    Escherichia coli (strain K12)
  • 蛋白長度：
    Full Length of Mature Protein
  • 表達區域：
    45-334
  • 氨基酸序列
    DLLSWF KGNDRPPAPA GKALEFSKPA AWQNNLPLTP ADKVSGYNNF YEFGLDKADP AANAGSLKTD PWTLKISGEV AKPLTLDHDD LTRRFPLEER IYRMRCVEAW SMVVPWIGFP LHKLLALAEP TSNAKYVAFE TIYAPEQMPG QQDRFIGGGL KYPYVEGLRL DEAMHPLTLM TVGVYGKALP PQNGAPVRLI VPWKYGFKGI KSIVSIKLTR ERPPTTWNLA APDEYGFYAN VNPYVDHPRW SQATERFIGS GGILDVQRQP TLLFNGYAAQ VASLYRGLDL RENF
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Part of the MsrPQ system that repairs oxidized periplasmic proteins containing methionine sulfoxide residues (Met-O), using respiratory chain electrons. Thus protects these proteins from oxidative-stress damage caused by reactive species of oxygen and chlorine. MsrPQ is essential for the maintenance of envelope integrity under bleach stress, rescuing a wide series of structurally unrelated periplasmic proteins from methionine oxidation, including the primary periplasmic chaperone SurA and the lipoprotein Pal. The catalytic subunit MsrP is non-stereospecific, being able to reduce both (R-) and (S-) diastereoisomers of methionine sulfoxide. Can catalyze the reduction of a variety of substrates in vitro, including dimethyl sulfoxide, trimethylamine N-oxide, phenylmethyl sulfoxide and L-methionine sulfoxide. Cannot reduce cyclic N-oxides. Shows no activity as sulfite oxidase.
  • 基因功能參考文獻：
    1. The study reports on the structural characterization using X-ray absorption spectroscopy of the molybdenum site of Escherichia coli YedY. PMID: 21190336
    2. Study investigated the atomic structure of the molybdenum site in YedY by X-ray absorption spectroscopy PMID: 21190337
    3. YedY and its many orthologues may represent a new type of membrane-associated bacterial reductase. PMID: 15355966
    4. YedY is a sulfite oxidase catalytic subunit with a twin arginine leader sequence for export to the periplasm by the Tat translocation system. PMID: 16042411
  • 亞細胞定位：
    Periplasm. Note=Is attached to the inner membrane when interacting with the MsrQ subunit.
  • 蛋白家族：
    MsrP family
  • 數據庫鏈接：

    KEGG: ecj:JW1954

    STRING: 316407.85675174



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