在线日韩日本国产亚洲丨少妇伦子伦情品无吗丨欧美性猛交xxxx免费看蜜桃丨精品人妻系列无码一区二区三区丨亚洲精品无码不卡在线播放

Your Good Partner in Biology Research

  1. 首頁
  2. 蛋白
  3. 重組蛋白
  4. Recombinant Escherichia coli Small-conductance mechanosensitive channel (mscS), partial

Recombinant Escherichia coli Small-conductance mechanosensitive channel (mscS), partial

  • 中文名稱：
    大腸桿菌mscS重組蛋白
  • 貨號：
    CSB-YP314762ENV1
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    大腸桿菌mscS重組蛋白
  • 貨號：
    CSB-EP314762ENV1
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    大腸桿菌mscS重組蛋白
  • 貨號：
    CSB-EP314762ENV1-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    大腸桿菌mscS重組蛋白
  • 貨號：
    CSB-BP314762ENV1
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    大腸桿菌mscS重組蛋白
  • 貨號：
    CSB-MP314762ENV1
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    mscS
  • Uniprot No.：
  • 別名：
    mscS; yggB; b2924; JW2891; Small-conductance mechanosensitive channel
  • 種屬：
    Escherichia coli (strain K12)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Mechanosensitive channel that participates in the regulation of osmotic pressure changes within the cell, opening in response to stretch forces in the membrane lipid bilayer, without the need for other proteins. Contributes to normal resistance to hypoosmotic shock. Forms an ion channel of 1.0 nanosiemens conductance with a slight preference for anions. The channel is sensitive to voltage; as the membrane is depolarized, less tension is required to open the channel and vice versa. The channel is characterized by short bursts of activity that last for a few seconds.
  • 基因功能參考文獻：
    1. inactivation process of wild-type MscS was strongly affected by voltage. The wild-type MscS inactivated at +60 to +80 mV but not at -60 to +40 mV. PMID: 28101504
    2. Mutations in a conserved domain of E. coli MscS to the most conserved superfamily residue leads to kinetic changes. PMID: 26340270
    3. Molecular dynamics and biophysical analyses show that the volume of the pockets and thus the number of lipid acyl chains within them decreases upon channel opening. PMID: 26551077
    4. Data show that trifluoroethanol (TFE) affects mechanosensitive channel of small conductance (MscS) channel gating kinetics in spheroplasts and liposomes. PMID: 26116700
    5. Data indicate that mechanosensitive channel of small conductance (MscS) protein opens in response to a relief of intrinsic lipid bilayer pressure. PMID: 25450806
    6. We report here crystal structures of MscS from E. coli and Helicobacter pylori solubilized in the detergent beta-dodecylmaltoside at resolutions of 4.4 and 4.2 A, respectively. PMID: 23339071
    7. Article reviews the science that led to the isolation and identification of MscS. structure-function relationship of the channel, in particular the structural and biochemical approaches to understanding channel gating. [Review] PMID: 22404681
    8. Data show that the MscL/MscS threshold ratio dramatically decreased in thinner compared with thicker bilayers. PMID: 22586095
    9. Tension dependencies of both inactivation and recovery rates suggest the spatial scale of the inactivating transition, which guides the modeling of the inactivated state of MscS. PMID: 21670207
    10. Both F68S and L111S in MscS substitutions produced severe loss-of-function phenotypes in vivo by increasing the inactivation rate and promoting unusual 'silent' inactivation from the resting state PMID: 20208543
    11. the crystal structure of MscS does not represent an open state; MscS gate involves a vapor-lock mechanism where limited changes of geometry or surface polarity can locally switch the regime between water-filled (conducting)& empty (nonconducting) states PMID: 15111405
    12. The crystal structure of MscS reveals a homoheptamer with three transmembrane segments and a large cytoplasmic domain PMID: 17500538
    13. Analysis of the fluorescence properties of purified mutant MscS proteins containing single Trp residues revealed that W16 and W251 are relatively inaccessible, whereas W240 is accessible to quenching agents. PMID: 17718516
    14. Transition to this unique mechanosensitive channel state is discussed in terms of existing data. PMID: 18065458
    15. study presents a 3.45 angstrom-resolution structure for the MscS channel in an open conformation; structure suggests a molecular mechanism that underlies MscS gating and its decay of conductivity during prolonged activation PMID: 18755969
    16. determination of structural rearrangements associated with MscS activation in membranes; transition from closed to open state is accompanied by downward tilting of the transmembrane TM1-TM2 hairpin & by expansion, tilt & rotation of the TM3 helices PMID: 18755978
    17. MscL and MscS are tangible models for studying conformational transitions in membrane proteins driven directly by membrane tension. PMID: 19383606

    顯示更多

    收起更多

  • 亞細胞定位：
    Cell inner membrane; Multi-pass membrane protein.
  • 蛋白家族：
    MscS (TC 1.A.23) family
  • 數據庫鏈接：


主站蜘蛛池模板： 国产精品久久婷婷六月丁香| 妓院一钑片免看黄大片| 亚洲精品一区二区三区蜜臀| 久久久久久亚洲精品成人| 亚洲乱码日产精品bd在| 亚洲日韩欧美一区二区三区| 国产精品熟女高潮视频| 欧美变态另类xxxx| 国产美女露脸口爆吞精| 国产日韩av在线播放| 最新中文字幕av无码专区| 成年无码动漫av片在线尤物| 亚洲五月综合缴情在线观看| 性做久久久久久久| 成人性生交大片免费看vr| 久久无码无码久久综合综合| 欧美z0zo人禽交免费观看99| 香港三日本三级少妇三级66| 777米奇影视第四色| 国精品人妻无码一区二区三区性色 | 天堂久久久久va久久久久| 亚洲五月天综合| 亚洲成av人片在一线观看| 国内精品美女a∨在线播放| 精品综合久久久久久8888 | 人妻 校园 激情 另类| 荡女精品导航| 国内无遮挡18禁无码网站免费| 国产农村妇女高潮大叫| 草草影院发布页| 青青国产揄拍视频| 天天爽夜夜爽人人爽88| 在线观看国产一区二区三区| h无码动漫在线观看| 丰满的少妇邻居中文bd| 狠狠色噜噜狠狠狠狠7777米奇| 精品人妻无码一区二区三区抖音| 啪啪激情婷婷久久婷婷色五月| 国产精品无码无卡在线播放| 红杏亚洲影院一区二区三区| 邻居少妇张开腿让我爽了一夜|