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  4. Recombinant Escherichia coli Signal recognition particle receptor FtsY (ftsY)

Recombinant Escherichia coli Signal recognition particle receptor FtsY (ftsY)

  • 中文名稱：
    大腸桿菌ftsY重組蛋白
  • 貨號：
    CSB-YP319158ENV
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    大腸桿菌ftsY重組蛋白
  • 貨號：
    CSB-EP319158ENV
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    大腸桿菌ftsY重組蛋白
  • 貨號：
    CSB-EP319158ENV-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    大腸桿菌ftsY重組蛋白
  • 貨號：
    CSB-BP319158ENV
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    大腸桿菌ftsY重組蛋白
  • 貨號：
    CSB-MP319158ENV
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    ftsY
  • Uniprot No.：
  • 別名：
    ftsY; b3464; JW3429Signal recognition particle receptor FtsY; SRP receptor
  • 種屬：
    Escherichia coli (strain K12)
  • 蛋白長度：
    Full Length of Mature Protein
  • 表達區域：
    2-497
  • 氨基酸序列
    AKEKKRGFF SWLGFGQKEQ TPEKETEVQN EQPVVEEIVQ AQEPVKASEQ AVEEQPQAHT EAEAETFAAD VVEVTEQVAE SEKAQPEAEV VAQPEPVVEE TPEPVAIERE ELPLPEDVNA EAVSPEEWQA EAETVEIVEA AEEEAAKEEI TDEELETALA AEAAEEAVMV VPPAEEEQPV EEIAQEQEKP TKEGFFARLK RSLLKTKENL GSGFISLFRG KKIDDDLFEE LEEQLLIADV GVETTRKIIT NLTEGASRKQ LRDAEALYGL LKEEMGEILA KVDEPLNVEG KAPFVILMVG VNGVGKTTTI GKLARQFEQQ GKSVMLAAGD TFRAAAVEQL QVWGQRNNIP VIAQHTGADS ASVIFDAIQA AKARNIDVLI ADTAGRLQNK SHLMEELKKI VRVMKKLDVE APHEVMLTID ASTGQNAVSQ AKLFHEAVGL TGITLTKLDG TAKGGVIFSV ADQFGIPIRY IGVGERIEDL RPFKADDFIE ALFARED
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Acts as a receptor for the complex formed by the signal recognition particle (SRP) and the ribosome-nascent chain (RNC). Interaction with SRP-RNC leads to the transfer of the RNC complex to the Sec translocase for insertion into the membrane, the hydrolysis of GTP by both Ffh and FtsY, and the dissociation of the SRP-FtsY complex into the individual components.
  • 基因功能參考文獻：
    1. FtsY can target the membrane co-translationally. PMID: 25653387
    2. analysis of the ribosome-SRP-FtsY cotranslational targeting complex in the closed state PMID: 25775537
    3. Data show that phospholipid binding drives important conformational rearrangements that activate the bacterial SRP receptor FtsY and the SRP-FtsY complex. PMID: 20733058
    4. FtsY directly interacts with the SecY translocon. PMID: 15815684
    5. Deletion experiments showed that this helix is essential for FtsY function in vivo, thus offering clear evidence for the functionally important, physiologically relevant interaction of FtsY with lipids. PMID: 17726012
    6. In conclusion, we propose that the SRP receptor, FtsY has two distinct and separable roles in (i) mediating membrane targeting and docking of ribosomes and (ii) promoting their productive release from the docking site. PMID: 17726013
    7. membrane binding and function of FtsY are in part regulated by proteolytic cleavage of the conserved 14-amino-acid motif. PMID: 18281057
    8. the SRP and SR GTPases, without recruiting external regulatory factors, constitute a self-sufficient system that provides exquisite spatial and temporal control of a complex cellular process. PMID: 19174514
    9. The data reveal that the two consecutive lipid-binding helices of FtsY can provide a membrane contact that does not differ significantly in stability from that provided by a transmembrane domain. PMID: 19414018

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  • 亞細胞定位：
    Cell inner membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm.
  • 蛋白家族：
    GTP-binding SRP family, FtsY subfamily
  • 數據庫鏈接：


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